Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city
Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo. The antigen ELISAs showed a high sensitivity (>84%), but the B158B60 Ag-ELISA appeared to be more specific than the HP10 Ag-ELISA (model 1: 95% vs 74%; model 2: 87% vs 71%). The overall prevalence of porcine cysticercosis in Antananarivo slaughterhouses was estimated at 2.3% (95% credibility interval [95%CrI]: 0.09–9.1%) to 2.6% (95%CrI: 0.1–10.3%) depending on the model and priors used. Since the sample used in this study is not representative of the national pig population, village-based surveys and longitudinal monitoring at slaughter are needed to better estimate the overall prevalence, geographical patterns and main risk factors for T. solium contamination, in order to improve control policies.
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dig-cirad-fr-5772272024-01-28T22:50:35Z http://agritrop.cirad.fr/577227/ http://agritrop.cirad.fr/577227/ Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city. Porphyre Vincent, Betson M., Rabezanahary H., Mboussou Yoan, Zafindraibe N.J., Rasamoelina-Andriamanivo Harentsoaniaina, Costard Solenne, Pfeiffer Dirk Udo, Michault Alain. 2016. Veterinary Parasitology, 219 : 77-83.https://doi.org/10.1016/j.vetpar.2015.08.027 <https://doi.org/10.1016/j.vetpar.2015.08.027> Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city Porphyre, Vincent Betson, M. Rabezanahary, H. Mboussou, Yoan Zafindraibe, N.J. Rasamoelina-Andriamanivo, Harentsoaniaina Costard, Solenne Pfeiffer, Dirk Udo Michault, Alain eng 2016 Veterinary Parasitology L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 Madagascar http://aims.fao.org/aos/agrovoc/c_4510 Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo. The antigen ELISAs showed a high sensitivity (>84%), but the B158B60 Ag-ELISA appeared to be more specific than the HP10 Ag-ELISA (model 1: 95% vs 74%; model 2: 87% vs 71%). The overall prevalence of porcine cysticercosis in Antananarivo slaughterhouses was estimated at 2.3% (95% credibility interval [95%CrI]: 0.09–9.1%) to 2.6% (95%CrI: 0.1–10.3%) depending on the model and priors used. Since the sample used in this study is not representative of the national pig population, village-based surveys and longitudinal monitoring at slaughter are needed to better estimate the overall prevalence, geographical patterns and main risk factors for T. solium contamination, in order to improve control policies. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/577227/7/577227.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1016/j.vetpar.2015.08.027 10.1016/j.vetpar.2015.08.027 info:eu-repo/semantics/altIdentifier/doi/10.1016/j.vetpar.2015.08.027 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1016/j.vetpar.2015.08.027 |
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L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 http://aims.fao.org/aos/agrovoc/c_4510 L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 http://aims.fao.org/aos/agrovoc/c_4510 |
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L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 http://aims.fao.org/aos/agrovoc/c_4510 L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 http://aims.fao.org/aos/agrovoc/c_4510 Porphyre, Vincent Betson, M. Rabezanahary, H. Mboussou, Yoan Zafindraibe, N.J. Rasamoelina-Andriamanivo, Harentsoaniaina Costard, Solenne Pfeiffer, Dirk Udo Michault, Alain Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
description |
Taenia solium cysticercosis was reported in official veterinary and medical statistics to be highly prevalent in pigs and humans in Madagascar, but few estimates are available for pigs. This study aimed to estimate the seroprevalence of porcine cysticercosis among pigs slaughtered in Antananarivo abattoirs. Firstly, the diagnostic performance of two antigen-ELISA techniques (B158B60 Ag-ELISA and HP10 Ag-ELISA) and an immunoblotting method were compared with meat inspection procedures on a sample of pigs suspected to be infected with (group 1; n = 250) or free of (group 2; n = 250) T. solium based on direct veterinary inspection in Madagascar. Sensitivity and specificity of the antigen ELISAs were then estimated using a Bayesian approach for detection of porcine cysticercosis in the absence of a gold standard. Then, a third set of pig sera (group 3, n = 250) was randomly collected in Antananarivo slaughterhouses and tested to estimate the overall prevalence of T. solium contamination in pork meat traded in Antananarivo. The antigen ELISAs showed a high sensitivity (>84%), but the B158B60 Ag-ELISA appeared to be more specific than the HP10 Ag-ELISA (model 1: 95% vs 74%; model 2: 87% vs 71%). The overall prevalence of porcine cysticercosis in Antananarivo slaughterhouses was estimated at 2.3% (95% credibility interval [95%CrI]: 0.09–9.1%) to 2.6% (95%CrI: 0.1–10.3%) depending on the model and priors used. Since the sample used in this study is not representative of the national pig population, village-based surveys and longitudinal monitoring at slaughter are needed to better estimate the overall prevalence, geographical patterns and main risk factors for T. solium contamination, in order to improve control policies. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux U30 - Méthodes de recherche viande porcine Taenia solium cysticercose immunodiagnostic Test ELISA carcasse porcin morbidité abattoir Enquête organismes nuisibles technique analytique inspection des viandes http://aims.fao.org/aos/agrovoc/c_6120 http://aims.fao.org/aos/agrovoc/c_31970 http://aims.fao.org/aos/agrovoc/c_2085 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_15968 http://aims.fao.org/aos/agrovoc/c_1310 http://aims.fao.org/aos/agrovoc/c_7555 http://aims.fao.org/aos/agrovoc/c_4936 http://aims.fao.org/aos/agrovoc/c_8 http://aims.fao.org/aos/agrovoc/c_32767 http://aims.fao.org/aos/agrovoc/c_1513 http://aims.fao.org/aos/agrovoc/c_4676 http://aims.fao.org/aos/agrovoc/c_4510 |
author |
Porphyre, Vincent Betson, M. Rabezanahary, H. Mboussou, Yoan Zafindraibe, N.J. Rasamoelina-Andriamanivo, Harentsoaniaina Costard, Solenne Pfeiffer, Dirk Udo Michault, Alain |
author_facet |
Porphyre, Vincent Betson, M. Rabezanahary, H. Mboussou, Yoan Zafindraibe, N.J. Rasamoelina-Andriamanivo, Harentsoaniaina Costard, Solenne Pfeiffer, Dirk Udo Michault, Alain |
author_sort |
Porphyre, Vincent |
title |
Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
title_short |
Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
title_full |
Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
title_fullStr |
Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
title_full_unstemmed |
Taenia solium porcine cysticercosis in Madagascar: Comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in Antananarivo city |
title_sort |
taenia solium porcine cysticercosis in madagascar: comparison of immuno-diagnostic techniques and estimation of the prevalence in pork carcasses traded in antananarivo city |
url |
http://agritrop.cirad.fr/577227/ http://agritrop.cirad.fr/577227/7/577227.pdf |
work_keys_str_mv |
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