Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana
Background The African buffalo (Syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. Theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-Saharan Africa, resulting in extensive economic losses to livestock farmers in endemic areas. Information on the distribution of tick-borne diseases and ticks is scarce in Northern Botswana. Nevertheless, this data is necessary for targeting surveillance and control measures in livestock production at national level. Methods In order to address this gap, we analyzed 120 blood samples from buffalo herds for the presence of common tick-borne haemoparasites causing disease in livestock, collected in two of the main wildlife areas of Northern Botswana: the Chobe National Park (CNP, n?=?64) and the Okavango Delta (OD, n?=?56). Results Analysis of the reverse line blot (RLB) hybridization assay results revealed the presence of Theileria, Babesia, Anaplasma and Ehrlichia species, either as single or mixed infections. Among the Theileria spp. present, T. parva (60%) and T. mutans (37%) were the most prevalent. Other species of interest were Anaplasma marginale subsp. centrale (30%), A. marginale (20%), Babesia occultans (23%) and Ehrlichia ruminantium (6%). The indirect fluorescent antibody test (IFAT) indicated 74% of samples to be positive for the presence of T. parva antibodies. Quantitative real-time PCR (qPCR) detected the highest level of animals infected with T. parva (81% of the samples). The level of agreement between the tests for detection of T. parva positive animals was higher between qPCR and IFAT (kappa?=?0.56), than between qPCR and RLB (kappa?=?0.26) or the latter and IFAT (kappa?=?0.15). Conclusions This is the first report of tick-borne haemoparasites in African buffalo from northern Botswana, where animals from the CNP showed higher levels of infection than those from OD. Considering the absence of fences separating wildlife and livestock in the CNP and the higher levels of some parasite species in buffalo from that area, surveillance of tick-borne diseases in livestock at the interface in the CNP should be prioritized.
id |
dig-cirad-fr-574881 |
---|---|
record_format |
koha |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 http://aims.fao.org/aos/agrovoc/c_1030 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 http://aims.fao.org/aos/agrovoc/c_1030 |
spellingShingle |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 http://aims.fao.org/aos/agrovoc/c_1030 L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 http://aims.fao.org/aos/agrovoc/c_1030 Eygelaar, Dewald Jori, Ferran Mokopasetso, Mokganedi Sibeko, Kgomotso P. Collins, Nicola E. Vorster, Ilse Troskie, Milana Oosthuizen, Marinda C. Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
description |
Background The African buffalo (Syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. Theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-Saharan Africa, resulting in extensive economic losses to livestock farmers in endemic areas. Information on the distribution of tick-borne diseases and ticks is scarce in Northern Botswana. Nevertheless, this data is necessary for targeting surveillance and control measures in livestock production at national level. Methods In order to address this gap, we analyzed 120 blood samples from buffalo herds for the presence of common tick-borne haemoparasites causing disease in livestock, collected in two of the main wildlife areas of Northern Botswana: the Chobe National Park (CNP, n?=?64) and the Okavango Delta (OD, n?=?56). Results Analysis of the reverse line blot (RLB) hybridization assay results revealed the presence of Theileria, Babesia, Anaplasma and Ehrlichia species, either as single or mixed infections. Among the Theileria spp. present, T. parva (60%) and T. mutans (37%) were the most prevalent. Other species of interest were Anaplasma marginale subsp. centrale (30%), A. marginale (20%), Babesia occultans (23%) and Ehrlichia ruminantium (6%). The indirect fluorescent antibody test (IFAT) indicated 74% of samples to be positive for the presence of T. parva antibodies. Quantitative real-time PCR (qPCR) detected the highest level of animals infected with T. parva (81% of the samples). The level of agreement between the tests for detection of T. parva positive animals was higher between qPCR and IFAT (kappa?=?0.56), than between qPCR and RLB (kappa?=?0.26) or the latter and IFAT (kappa?=?0.15). Conclusions This is the first report of tick-borne haemoparasites in African buffalo from northern Botswana, where animals from the CNP showed higher levels of infection than those from OD. Considering the absence of fences separating wildlife and livestock in the CNP and the higher levels of some parasite species in buffalo from that area, surveillance of tick-borne diseases in livestock at the interface in the CNP should be prioritized. |
format |
article |
topic_facet |
L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 http://aims.fao.org/aos/agrovoc/c_1030 |
author |
Eygelaar, Dewald Jori, Ferran Mokopasetso, Mokganedi Sibeko, Kgomotso P. Collins, Nicola E. Vorster, Ilse Troskie, Milana Oosthuizen, Marinda C. |
author_facet |
Eygelaar, Dewald Jori, Ferran Mokopasetso, Mokganedi Sibeko, Kgomotso P. Collins, Nicola E. Vorster, Ilse Troskie, Milana Oosthuizen, Marinda C. |
author_sort |
Eygelaar, Dewald |
title |
Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
title_short |
Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
title_full |
Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
title_fullStr |
Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
title_full_unstemmed |
Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana |
title_sort |
tick-borne haemoparasites in african buffalo (syncerus caffer) from two wildlife areas in northern botswana |
url |
http://agritrop.cirad.fr/574881/ http://agritrop.cirad.fr/574881/1/document_574881.pdf |
work_keys_str_mv |
AT eygelaardewald tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT joriferran tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT mokopasetsomokganedi tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT sibekokgomotsop tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT collinsnicolae tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT vorsterilse tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT troskiemilana tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana AT oosthuizenmarindac tickbornehaemoparasitesinafricanbuffalosynceruscafferfromtwowildlifeareasinnorthernbotswana |
_version_ |
1792498745259065344 |
spelling |
dig-cirad-fr-5748812024-01-28T22:25:13Z http://agritrop.cirad.fr/574881/ http://agritrop.cirad.fr/574881/ Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana. Eygelaar Dewald, Jori Ferran, Mokopasetso Mokganedi, Sibeko Kgomotso P., Collins Nicola E., Vorster Ilse, Troskie Milana, Oosthuizen Marinda C.. 2015. Parasites and Vectors, 8 (26), 11 p.https://doi.org/10.1186/s13071-014-0627-y <https://doi.org/10.1186/s13071-014-0627-y> Tick-borne haemoparasites in African buffalo (Syncerus caffer) from two wildlife areas in Northern Botswana Eygelaar, Dewald Jori, Ferran Mokopasetso, Mokganedi Sibeko, Kgomotso P. Collins, Nicola E. Vorster, Ilse Troskie, Milana Oosthuizen, Marinda C. eng 2015 Parasites and Vectors L72 - Organismes nuisibles des animaux L73 - Maladies des animaux buffle africain parasite maladie transmissible par tiques maladie transmise par vecteur Enquête pathologique Theileria Babesia Anaplasma Ehrlichia sang animal sauvage animal domestique immunodiagnostic PCR immunofluorescence http://aims.fao.org/aos/agrovoc/c_167 http://aims.fao.org/aos/agrovoc/c_5574 http://aims.fao.org/aos/agrovoc/c_24908 http://aims.fao.org/aos/agrovoc/c_34142 http://aims.fao.org/aos/agrovoc/c_28665 http://aims.fao.org/aos/agrovoc/c_7708 http://aims.fao.org/aos/agrovoc/c_755 http://aims.fao.org/aos/agrovoc/c_388 http://aims.fao.org/aos/agrovoc/c_2504 http://aims.fao.org/aos/agrovoc/c_959 http://aims.fao.org/aos/agrovoc/c_24103 http://aims.fao.org/aos/agrovoc/c_2356 http://aims.fao.org/aos/agrovoc/c_27555 http://aims.fao.org/aos/agrovoc/c_34079 http://aims.fao.org/aos/agrovoc/c_3804 Botswana http://aims.fao.org/aos/agrovoc/c_1030 Background The African buffalo (Syncerus caffer) is a host for many pathogens known to cause economically important diseases and is often considered an important reservoir for livestock diseases. Theileriosis, heartwater, babesiosis and anaplasmosis are considered the most important tick-borne diseases of livestock in sub-Saharan Africa, resulting in extensive economic losses to livestock farmers in endemic areas. Information on the distribution of tick-borne diseases and ticks is scarce in Northern Botswana. Nevertheless, this data is necessary for targeting surveillance and control measures in livestock production at national level. Methods In order to address this gap, we analyzed 120 blood samples from buffalo herds for the presence of common tick-borne haemoparasites causing disease in livestock, collected in two of the main wildlife areas of Northern Botswana: the Chobe National Park (CNP, n?=?64) and the Okavango Delta (OD, n?=?56). Results Analysis of the reverse line blot (RLB) hybridization assay results revealed the presence of Theileria, Babesia, Anaplasma and Ehrlichia species, either as single or mixed infections. Among the Theileria spp. present, T. parva (60%) and T. mutans (37%) were the most prevalent. Other species of interest were Anaplasma marginale subsp. centrale (30%), A. marginale (20%), Babesia occultans (23%) and Ehrlichia ruminantium (6%). The indirect fluorescent antibody test (IFAT) indicated 74% of samples to be positive for the presence of T. parva antibodies. Quantitative real-time PCR (qPCR) detected the highest level of animals infected with T. parva (81% of the samples). The level of agreement between the tests for detection of T. parva positive animals was higher between qPCR and IFAT (kappa?=?0.56), than between qPCR and RLB (kappa?=?0.26) or the latter and IFAT (kappa?=?0.15). Conclusions This is the first report of tick-borne haemoparasites in African buffalo from northern Botswana, where animals from the CNP showed higher levels of infection than those from OD. Considering the absence of fences separating wildlife and livestock in the CNP and the higher levels of some parasite species in buffalo from that area, surveillance of tick-borne diseases in livestock at the interface in the CNP should be prioritized. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/574881/1/document_574881.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1186/s13071-014-0627-y 10.1186/s13071-014-0627-y info:eu-repo/semantics/altIdentifier/doi/10.1186/s13071-014-0627-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1186/s13071-014-0627-y |