Antioxidant and anti-inflammatory in vitro activities of phenolic compounds from tropical highland blackberry (Rubus adenotrichos)

This study evaluates the antioxidant and anti-inflammatory activities in a polyphenol extract from blackberries. The antioxidant activity measured via oxygen radical absorbance capacity (ORAC) was higher for the blackberry extract (4339 ± 144 ?M TE/g) than for quercetin and ellagic acid. The blackberry phenolic compounds protected liposomes and liver homogenates against lipid peroxidation; in both models, the antioxidant activity (IC50 = 7.0 ± 0.5 and 20.3 ± 4.2 ?g/mL, respectively) was greater than that found with Trolox. The extract inhibited superoxide production by NADPH oxidase in THP-1 cells and nitrite production in J774A.1 cells stimulated with LPS+IFN?, with nitrite production decreasing after 4 h of incubation with the extract, mainly through a strong scavenging activity. However, 24 h of treatment reduced the amount of nitrites (IC50 = 45.6 ± 1.2 ?g/mL) because of a down-regulation of iNOS protein expression, as demonstrated by Western blotting. The inhibitory activities found in blackberry phenols suggest a potential beneficial effect against oxidative stress and inflammatory processes.

Bibliographic Details

Main Authors:	Azofeifa, Gabriela, Quesada, Silvia, Boudard, Frédéric, Morena, Marion, Cristol, Jean-Paul, Pérez, Ana Mercedes, Vaillant, Fabrice, Michel, Alain
Format:	article biblioteca
Language:	eng
Subjects:	Q04 - Composition des produits alimentaires, L51 - Physiologie animale - Nutrition, F60 - Physiologie et biochimie végétale, 000 - Autres thèmes, mûre, Rubus, antioxydant, anti-inflammatoire, oxydation, lipide, composé phénolique, tannin, stress, http://aims.fao.org/aos/agrovoc/c_25487, http://aims.fao.org/aos/agrovoc/c_6682, http://aims.fao.org/aos/agrovoc/c_511, http://aims.fao.org/aos/agrovoc/c_507, http://aims.fao.org/aos/agrovoc/c_5472, http://aims.fao.org/aos/agrovoc/c_4362, http://aims.fao.org/aos/agrovoc/c_5772, http://aims.fao.org/aos/agrovoc/c_7606, http://aims.fao.org/aos/agrovoc/c_7452, http://aims.fao.org/aos/agrovoc/c_1920,
Online Access:	http://agritrop.cirad.fr/570783/ http://agritrop.cirad.fr/570783/1/document_570783.pdf
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