XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK
Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc.
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Subjects: | H20 - Maladies des plantes, F30 - Génétique et amélioration des plantes, Xanthomonas campestris, écotype, Pseudomonas syringae, Ralstonia solanacearum, Arabidopsis, pouvoir pathogène, résistance aux maladies, cytoplasme, immunité, transférase, activité enzymatique, http://aims.fao.org/aos/agrovoc/c_24379, http://aims.fao.org/aos/agrovoc/c_27505, http://aims.fao.org/aos/agrovoc/c_26650, http://aims.fao.org/aos/agrovoc/c_37076, http://aims.fao.org/aos/agrovoc/c_33291, http://aims.fao.org/aos/agrovoc/c_5629, http://aims.fao.org/aos/agrovoc/c_2328, http://aims.fao.org/aos/agrovoc/c_2093, http://aims.fao.org/aos/agrovoc/c_3802, http://aims.fao.org/aos/agrovoc/c_7865, http://aims.fao.org/aos/agrovoc/c_2604, |
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dig-cirad-fr-5703222024-01-28T21:36:10Z http://agritrop.cirad.fr/570322/ http://agritrop.cirad.fr/570322/ XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK. Guy Endrick, Lautier Martine, Chabannes Matthieu, Roux Brice, Lauber Emmanuelle, Arlat Mathieu, Noel Laurent D.. 2013. PloS One, 8 (8):e73469, 13 p.https://doi.org/10.1371/journal.pone.0073469 <https://doi.org/10.1371/journal.pone.0073469> XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK Guy, Endrick Lautier, Martine Chabannes, Matthieu Roux, Brice Lauber, Emmanuelle Arlat, Mathieu Noel, Laurent D. eng 2013 PloS One H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/570322/1/document_570322.pdf application/pdf Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1371/journal.pone.0073469 10.1371/journal.pone.0073469 info:eu-repo/semantics/altIdentifier/doi/10.1371/journal.pone.0073469 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1371/journal.pone.0073469 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 |
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H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 Guy, Endrick Lautier, Martine Chabannes, Matthieu Roux, Brice Lauber, Emmanuelle Arlat, Mathieu Noel, Laurent D. XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
description |
Xanthomonas campestris pv. campestris (Xcc) colonizes the vascular system of Brassicaceae and ultimately causes black rot. In susceptible Arabidopsis plants, XopAC type III effector inhibits by uridylylation positive regulators of the PAMP-triggered immunity such as the receptor-like cytoplasmic kinases (RLCK) BIK1 and PBL1. In the resistant ecotype Col-0, xopAC is a major avirulence gene of Xcc. In this study, we show that both the RLCK interaction domain and the uridylyl transferase domain of XopAC are required for avirulence. Furthermore, xopAC can also confer avirulence to both the vascular pathogen Ralstonia solanacearum and the mesophyll-colonizing pathogen Pseudomonas syringae indicating that xopAC-specified effector-triggered immunity is not specific to the vascular system. In planta, XopAC-YFP fusions are localized at the plasma membrane suggesting that XopAC might interact with membrane-localized proteins. Eight RLCK of subfamily VII predicted to be localized at the plasma membrane and interacting with XopAC in yeast two-hybrid assays have been isolated. Within this subfamily, PBL2 and RIPK RLCK genes but not BIK1 are important for xopAC-specified effector-triggered immunity and Arabidopsis resistance to Xcc. |
format |
article |
topic_facet |
H20 - Maladies des plantes F30 - Génétique et amélioration des plantes Xanthomonas campestris écotype Pseudomonas syringae Ralstonia solanacearum Arabidopsis pouvoir pathogène résistance aux maladies cytoplasme immunité transférase activité enzymatique http://aims.fao.org/aos/agrovoc/c_24379 http://aims.fao.org/aos/agrovoc/c_27505 http://aims.fao.org/aos/agrovoc/c_26650 http://aims.fao.org/aos/agrovoc/c_37076 http://aims.fao.org/aos/agrovoc/c_33291 http://aims.fao.org/aos/agrovoc/c_5629 http://aims.fao.org/aos/agrovoc/c_2328 http://aims.fao.org/aos/agrovoc/c_2093 http://aims.fao.org/aos/agrovoc/c_3802 http://aims.fao.org/aos/agrovoc/c_7865 http://aims.fao.org/aos/agrovoc/c_2604 |
author |
Guy, Endrick Lautier, Martine Chabannes, Matthieu Roux, Brice Lauber, Emmanuelle Arlat, Mathieu Noel, Laurent D. |
author_facet |
Guy, Endrick Lautier, Martine Chabannes, Matthieu Roux, Brice Lauber, Emmanuelle Arlat, Mathieu Noel, Laurent D. |
author_sort |
Guy, Endrick |
title |
XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
title_short |
XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
title_full |
XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
title_fullStr |
XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
title_full_unstemmed |
XopAC-triggered Immunity against Xanthomonas depends on Arabidopsis receptor-like cytoplasmic kinase genes PBL2 and RIPK |
title_sort |
xopac-triggered immunity against xanthomonas depends on arabidopsis receptor-like cytoplasmic kinase genes pbl2 and ripk |
url |
http://agritrop.cirad.fr/570322/ http://agritrop.cirad.fr/570322/1/document_570322.pdf |
work_keys_str_mv |
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_version_ |
1792498514491604992 |