Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae)
Sixteen new polymorphic microsatellite loci were developed for the three species of the Ceratitis FAR complex (C. rosa, C. fasciventris and C. anonae) and their optimal amplification conditions characterised. These markers were tested on six field populations of the three fruit flies sampled in different African countries. After Bonferroni corrections, allele frequencies supported Hardy-Weinberg equilibrium for most of the loci, with only two loci not being at the equilibrium in all populations. No linkage disequilibrium was detected among all pairs of loci tested. These new markers will be a useful tool to study the intra- and interspecific genetic structure of these African agricultural pests and will help disentangling their well-known classification issues.
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dig-cirad-fr-5674572024-01-28T21:09:02Z http://agritrop.cirad.fr/567457/ http://agritrop.cirad.fr/567457/ Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae). Delatte Hélène, Virgilio Massimiliano, Simiand Christophe, Quilici Serge, De Meyer Marc. 2013. Conservation Genetics Resources, 5 (1) : 31-34.https://doi.org/10.1007/s12686-012-9722-6 <https://doi.org/10.1007/s12686-012-9722-6> Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) Delatte, Hélène Virgilio, Massimiliano Simiand, Christophe Quilici, Serge De Meyer, Marc eng 2013 Conservation Genetics Resources H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 Mozambique Kenya Afrique du Sud Rwanda République-Unie de Tanzanie Zambie Bénin Mali Ouganda République démocratique du Congo Cameroun http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 Sixteen new polymorphic microsatellite loci were developed for the three species of the Ceratitis FAR complex (C. rosa, C. fasciventris and C. anonae) and their optimal amplification conditions characterised. These markers were tested on six field populations of the three fruit flies sampled in different African countries. After Bonferroni corrections, allele frequencies supported Hardy-Weinberg equilibrium for most of the loci, with only two loci not being at the equilibrium in all populations. No linkage disequilibrium was detected among all pairs of loci tested. These new markers will be a useful tool to study the intra- and interspecific genetic structure of these African agricultural pests and will help disentangling their well-known classification issues. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/567457/1/dk567457.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s12686-012-9722-6 10.1007/s12686-012-9722-6 info:eu-repo/semantics/altIdentifier/doi/10.1007/s12686-012-9722-6 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s12686-012-9722-6 |
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H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 |
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H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 Delatte, Hélène Virgilio, Massimiliano Simiand, Christophe Quilici, Serge De Meyer, Marc Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
description |
Sixteen new polymorphic microsatellite loci were developed for the three species of the Ceratitis FAR complex (C. rosa, C. fasciventris and C. anonae) and their optimal amplification conditions characterised. These markers were tested on six field populations of the three fruit flies sampled in different African countries. After Bonferroni corrections, allele frequencies supported Hardy-Weinberg equilibrium for most of the loci, with only two loci not being at the equilibrium in all populations. No linkage disequilibrium was detected among all pairs of loci tested. These new markers will be a useful tool to study the intra- and interspecific genetic structure of these African agricultural pests and will help disentangling their well-known classification issues. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes U30 - Méthodes de recherche Ceratitis microsatellite marqueur génétique ravageur des plantes identification isolement culture fruitière culture maraîchère Mangifera indica http://aims.fao.org/aos/agrovoc/c_1462 http://aims.fao.org/aos/agrovoc/c_36574 http://aims.fao.org/aos/agrovoc/c_24030 http://aims.fao.org/aos/agrovoc/c_16196 http://aims.fao.org/aos/agrovoc/c_3791 http://aims.fao.org/aos/agrovoc/c_37744 http://aims.fao.org/aos/agrovoc/c_25476 http://aims.fao.org/aos/agrovoc/c_16509 http://aims.fao.org/aos/agrovoc/c_4575 http://aims.fao.org/aos/agrovoc/c_4964 http://aims.fao.org/aos/agrovoc/c_4086 http://aims.fao.org/aos/agrovoc/c_7252 http://aims.fao.org/aos/agrovoc/c_6717 http://aims.fao.org/aos/agrovoc/c_7608 http://aims.fao.org/aos/agrovoc/c_8501 http://aims.fao.org/aos/agrovoc/c_875 http://aims.fao.org/aos/agrovoc/c_4540 http://aims.fao.org/aos/agrovoc/c_8038 http://aims.fao.org/aos/agrovoc/c_8500 http://aims.fao.org/aos/agrovoc/c_1229 |
author |
Delatte, Hélène Virgilio, Massimiliano Simiand, Christophe Quilici, Serge De Meyer, Marc |
author_facet |
Delatte, Hélène Virgilio, Massimiliano Simiand, Christophe Quilici, Serge De Meyer, Marc |
author_sort |
Delatte, Hélène |
title |
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
title_short |
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
title_full |
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
title_fullStr |
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
title_full_unstemmed |
Isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three African agricultural pests (Ceratitis rosa, C. anonae and C. fasciventris, Diptera: Tephritidae) |
title_sort |
isolation and characterisation of sixteen microsatellite markers cross-amplifying in a complex of three african agricultural pests (ceratitis rosa, c. anonae and c. fasciventris, diptera: tephritidae) |
url |
http://agritrop.cirad.fr/567457/ http://agritrop.cirad.fr/567457/1/dk567457.pdf |
work_keys_str_mv |
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