Mutator system derivatives isolated from sugarcane genome sequence
Mutator-like transposase is the most represented transposon transcript in the sugarcane transcriptome. Phylogenetic reconstructions derived from sequenced transcripts provided evidence that at least four distinct classes exist (I- IV) and that diversification among these classes occurred early in Angiosperms, prior to the divergence of Monocots/ Eudicots. The four previously described classes served as probes to select and further sequence six BAC clones from a genomic library of cultivar R570. A total of 579,352 sugarcane base pairs were produced from these "Mutator system" BAC containing regions for further characterization. The analyzed genomic regions confirmed that the predicted structure and organization of the Mutator system in sugarcane is composed of two true transposon lineages, each containing a specific terminal inverted repeat and two transposase lineages considered to be domesticated. Each Mutator transposase class displayed a particular molecular structure supporting lineage specific evolution. MUSTANG, previously described domesticated genes, are located in syntenic regions across Sacharineae and, as expected for a host functional gene, posses the same gene structure as in other Poaceae. Two sequenced BACs correspond to hom(eo)logous locus with specific retrotransposon insertions that discriminate sugarcane haplotypes. The comparative studies presented, add information to the Mutator systems previously identified in the maize and rice genomes by describing lineage specific molecular structure and genomic distribution pattern in the sugarcane genome.
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Subjects: | F30 - Génétique et amélioration des plantes, Saccharum, génie génétique, Saccharum officinarum, Saccharum spontaneum, phylogénie, Poaceae, transcription, adn, génome, localisation de gène, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_15974, http://aims.fao.org/aos/agrovoc/c_6727, http://aims.fao.org/aos/agrovoc/c_6729, http://aims.fao.org/aos/agrovoc/c_13325, http://aims.fao.org/aos/agrovoc/c_3354, http://aims.fao.org/aos/agrovoc/c_35126, http://aims.fao.org/aos/agrovoc/c_2347, http://aims.fao.org/aos/agrovoc/c_3224, http://aims.fao.org/aos/agrovoc/c_24845, |
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dig-cirad-fr-5651592024-01-28T20:39:34Z http://agritrop.cirad.fr/565159/ http://agritrop.cirad.fr/565159/ Mutator system derivatives isolated from sugarcane genome sequence. Manetti M.E., Rossi Magdalena, Cruz Guilherme Marcelo Queiroga, Saccaro N.L., Nakabashi M., Altebarmakian V., Rodier-Goud Marguerite, Domingues Douglas S., D'Hont Angélique, Van Sluys Marie-Anne. 2012. Tropical Plant Biology, 5 (3) : 233-243.https://doi.org/10.1007/s12042-012-9104-y <https://doi.org/10.1007/s12042-012-9104-y> Mutator system derivatives isolated from sugarcane genome sequence Manetti, M.E. Rossi, Magdalena Cruz, Guilherme Marcelo Queiroga Saccaro, N.L. Nakabashi, M. Altebarmakian, V. Rodier-Goud, Marguerite Domingues, Douglas S. D'Hont, Angélique Van Sluys, Marie-Anne eng 2012 Tropical Plant Biology F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 Mutator-like transposase is the most represented transposon transcript in the sugarcane transcriptome. Phylogenetic reconstructions derived from sequenced transcripts provided evidence that at least four distinct classes exist (I- IV) and that diversification among these classes occurred early in Angiosperms, prior to the divergence of Monocots/ Eudicots. The four previously described classes served as probes to select and further sequence six BAC clones from a genomic library of cultivar R570. A total of 579,352 sugarcane base pairs were produced from these "Mutator system" BAC containing regions for further characterization. The analyzed genomic regions confirmed that the predicted structure and organization of the Mutator system in sugarcane is composed of two true transposon lineages, each containing a specific terminal inverted repeat and two transposase lineages considered to be domesticated. Each Mutator transposase class displayed a particular molecular structure supporting lineage specific evolution. MUSTANG, previously described domesticated genes, are located in syntenic regions across Sacharineae and, as expected for a host functional gene, posses the same gene structure as in other Poaceae. Two sequenced BACs correspond to hom(eo)logous locus with specific retrotransposon insertions that discriminate sugarcane haplotypes. The comparative studies presented, add information to the Mutator systems previously identified in the maize and rice genomes by describing lineage specific molecular structure and genomic distribution pattern in the sugarcane genome. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/565159/1/document_565159.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s12042-012-9104-y 10.1007/s12042-012-9104-y info:eu-repo/semantics/altIdentifier/doi/10.1007/s12042-012-9104-y info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s12042-012-9104-y |
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F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 |
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F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 Manetti, M.E. Rossi, Magdalena Cruz, Guilherme Marcelo Queiroga Saccaro, N.L. Nakabashi, M. Altebarmakian, V. Rodier-Goud, Marguerite Domingues, Douglas S. D'Hont, Angélique Van Sluys, Marie-Anne Mutator system derivatives isolated from sugarcane genome sequence |
description |
Mutator-like transposase is the most represented transposon transcript in the sugarcane transcriptome. Phylogenetic reconstructions derived from sequenced transcripts provided evidence that at least four distinct classes exist (I- IV) and that diversification among these classes occurred early in Angiosperms, prior to the divergence of Monocots/ Eudicots. The four previously described classes served as probes to select and further sequence six BAC clones from a genomic library of cultivar R570. A total of 579,352 sugarcane base pairs were produced from these "Mutator system" BAC containing regions for further characterization. The analyzed genomic regions confirmed that the predicted structure and organization of the Mutator system in sugarcane is composed of two true transposon lineages, each containing a specific terminal inverted repeat and two transposase lineages considered to be domesticated. Each Mutator transposase class displayed a particular molecular structure supporting lineage specific evolution. MUSTANG, previously described domesticated genes, are located in syntenic regions across Sacharineae and, as expected for a host functional gene, posses the same gene structure as in other Poaceae. Two sequenced BACs correspond to hom(eo)logous locus with specific retrotransposon insertions that discriminate sugarcane haplotypes. The comparative studies presented, add information to the Mutator systems previously identified in the maize and rice genomes by describing lineage specific molecular structure and genomic distribution pattern in the sugarcane genome. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Saccharum génie génétique Saccharum officinarum Saccharum spontaneum phylogénie Poaceae transcription adn génome localisation de gène http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_15974 http://aims.fao.org/aos/agrovoc/c_6727 http://aims.fao.org/aos/agrovoc/c_6729 http://aims.fao.org/aos/agrovoc/c_13325 http://aims.fao.org/aos/agrovoc/c_3354 http://aims.fao.org/aos/agrovoc/c_35126 http://aims.fao.org/aos/agrovoc/c_2347 http://aims.fao.org/aos/agrovoc/c_3224 http://aims.fao.org/aos/agrovoc/c_24845 |
author |
Manetti, M.E. Rossi, Magdalena Cruz, Guilherme Marcelo Queiroga Saccaro, N.L. Nakabashi, M. Altebarmakian, V. Rodier-Goud, Marguerite Domingues, Douglas S. D'Hont, Angélique Van Sluys, Marie-Anne |
author_facet |
Manetti, M.E. Rossi, Magdalena Cruz, Guilherme Marcelo Queiroga Saccaro, N.L. Nakabashi, M. Altebarmakian, V. Rodier-Goud, Marguerite Domingues, Douglas S. D'Hont, Angélique Van Sluys, Marie-Anne |
author_sort |
Manetti, M.E. |
title |
Mutator system derivatives isolated from sugarcane genome sequence |
title_short |
Mutator system derivatives isolated from sugarcane genome sequence |
title_full |
Mutator system derivatives isolated from sugarcane genome sequence |
title_fullStr |
Mutator system derivatives isolated from sugarcane genome sequence |
title_full_unstemmed |
Mutator system derivatives isolated from sugarcane genome sequence |
title_sort |
mutator system derivatives isolated from sugarcane genome sequence |
url |
http://agritrop.cirad.fr/565159/ http://agritrop.cirad.fr/565159/1/document_565159.pdf |
work_keys_str_mv |
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_version_ |
1792498253151862784 |