A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR)
Lignin content and composition are considered as mandatory traits of eucalyptus breeding programs, especially for pulp, paper, and bioenergy production. In this article, we used 33 Eucalyptus urophylla full-sib families of an 8× 8 factorial design to provide estimates of genetic parameters for lignin- and growth-related traits. Secondly, from the sequencing of the 16 unrelated founders, we described the nucleotide and haplotype variability of cinnamoyl-CoA reductase (CCR), a candidate gene for lignin-related traits encoding the cinnamoyl-CoA reductase. Finally, we tested the association between CCR polymorphisms and trait variation using a mixed linear model. A high value of narrow sense heritability was obtained for lignin content (h²=0.85) and S/G ratio (h²=0.62) indicating that these traits are under strong genetic control. High levels of nucleotide (??=0.0131) and haplotype (Hd=0.958) diversity were detected for CCR. From an initial set of 152 biallelic single nucleotide polymorphisms (SNPs), a subset of 65 nonredundant loci was selected. Three intronic SNPs were found to be associated to the variation of S/G ratio after multiple testing correction. In the line of what has been obtained in forest trees, these SNPs explained between 2.45% and 2.87% of the genetic variance of the trait. This study demonstrates the interest of the candidate gene approach for quantitative trait nucleotide detection in Eucalyptus and paves the way to gene assisted selection of lignin composition in E. urophylla.
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Subjects: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, K10 - Production forestière, Eucalyptus urophylla, lignine, gène, bois, oxydoréductase, http://aims.fao.org/aos/agrovoc/c_26492, http://aims.fao.org/aos/agrovoc/c_4329, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_8421, http://aims.fao.org/aos/agrovoc/c_5474, http://aims.fao.org/aos/agrovoc/c_1811, |
Online Access: | http://agritrop.cirad.fr/563834/ http://agritrop.cirad.fr/563834/1/document_563834.pdf |
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dig-cirad-fr-5638342024-01-28T20:18:33Z http://agritrop.cirad.fr/563834/ http://agritrop.cirad.fr/563834/ A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR). Mandrou Eric, Hein Paulo Ricardo Gherardi, Villar Emilie, Vigneron Philippe, Plomion Christophe, Gion Jean-Marc. 2012. Tree Genetics and Genomes, 8 (2) : 353-364.https://doi.org/10.1007/s11295-011-0446-7 <https://doi.org/10.1007/s11295-011-0446-7> A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) Mandrou, Eric Hein, Paulo Ricardo Gherardi Villar, Emilie Vigneron, Philippe Plomion, Christophe Gion, Jean-Marc eng 2012 Tree Genetics and Genomes F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 Congo http://aims.fao.org/aos/agrovoc/c_1811 Lignin content and composition are considered as mandatory traits of eucalyptus breeding programs, especially for pulp, paper, and bioenergy production. In this article, we used 33 Eucalyptus urophylla full-sib families of an 8× 8 factorial design to provide estimates of genetic parameters for lignin- and growth-related traits. Secondly, from the sequencing of the 16 unrelated founders, we described the nucleotide and haplotype variability of cinnamoyl-CoA reductase (CCR), a candidate gene for lignin-related traits encoding the cinnamoyl-CoA reductase. Finally, we tested the association between CCR polymorphisms and trait variation using a mixed linear model. A high value of narrow sense heritability was obtained for lignin content (h²=0.85) and S/G ratio (h²=0.62) indicating that these traits are under strong genetic control. High levels of nucleotide (??=0.0131) and haplotype (Hd=0.958) diversity were detected for CCR. From an initial set of 152 biallelic single nucleotide polymorphisms (SNPs), a subset of 65 nonredundant loci was selected. Three intronic SNPs were found to be associated to the variation of S/G ratio after multiple testing correction. In the line of what has been obtained in forest trees, these SNPs explained between 2.45% and 2.87% of the genetic variance of the trait. This study demonstrates the interest of the candidate gene approach for quantitative trait nucleotide detection in Eucalyptus and paves the way to gene assisted selection of lignin composition in E. urophylla. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/563834/1/document_563834.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s11295-011-0446-7 10.1007/s11295-011-0446-7 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11295-011-0446-7 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s11295-011-0446-7 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 http://aims.fao.org/aos/agrovoc/c_1811 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 http://aims.fao.org/aos/agrovoc/c_1811 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 http://aims.fao.org/aos/agrovoc/c_1811 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 http://aims.fao.org/aos/agrovoc/c_1811 Mandrou, Eric Hein, Paulo Ricardo Gherardi Villar, Emilie Vigneron, Philippe Plomion, Christophe Gion, Jean-Marc A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
description |
Lignin content and composition are considered as mandatory traits of eucalyptus breeding programs, especially for pulp, paper, and bioenergy production. In this article, we used 33 Eucalyptus urophylla full-sib families of an 8× 8 factorial design to provide estimates of genetic parameters for lignin- and growth-related traits. Secondly, from the sequencing of the 16 unrelated founders, we described the nucleotide and haplotype variability of cinnamoyl-CoA reductase (CCR), a candidate gene for lignin-related traits encoding the cinnamoyl-CoA reductase. Finally, we tested the association between CCR polymorphisms and trait variation using a mixed linear model. A high value of narrow sense heritability was obtained for lignin content (h²=0.85) and S/G ratio (h²=0.62) indicating that these traits are under strong genetic control. High levels of nucleotide (??=0.0131) and haplotype (Hd=0.958) diversity were detected for CCR. From an initial set of 152 biallelic single nucleotide polymorphisms (SNPs), a subset of 65 nonredundant loci was selected. Three intronic SNPs were found to be associated to the variation of S/G ratio after multiple testing correction. In the line of what has been obtained in forest trees, these SNPs explained between 2.45% and 2.87% of the genetic variance of the trait. This study demonstrates the interest of the candidate gene approach for quantitative trait nucleotide detection in Eucalyptus and paves the way to gene assisted selection of lignin composition in E. urophylla. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale K10 - Production forestière Eucalyptus urophylla lignine gène bois oxydoréductase http://aims.fao.org/aos/agrovoc/c_26492 http://aims.fao.org/aos/agrovoc/c_4329 http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_8421 http://aims.fao.org/aos/agrovoc/c_5474 http://aims.fao.org/aos/agrovoc/c_1811 |
author |
Mandrou, Eric Hein, Paulo Ricardo Gherardi Villar, Emilie Vigneron, Philippe Plomion, Christophe Gion, Jean-Marc |
author_facet |
Mandrou, Eric Hein, Paulo Ricardo Gherardi Villar, Emilie Vigneron, Philippe Plomion, Christophe Gion, Jean-Marc |
author_sort |
Mandrou, Eric |
title |
A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
title_short |
A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
title_full |
A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
title_fullStr |
A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
title_full_unstemmed |
A candidate gene for lignin composition in Eucalyptus: Cinnamoyl-CoA reductase (CCR) |
title_sort |
candidate gene for lignin composition in eucalyptus: cinnamoyl-coa reductase (ccr) |
url |
http://agritrop.cirad.fr/563834/ http://agritrop.cirad.fr/563834/1/document_563834.pdf |
work_keys_str_mv |
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