A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral)
Main Authors: | , , , , , , |
---|---|
Format: | conference_item biblioteca |
Language: | eng |
Published: |
SFP
|
Subjects: | H20 - Maladies des plantes, |
Online Access: | http://agritrop.cirad.fr/558646/ |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-558646 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-5586462019-04-12T16:05:49Z http://agritrop.cirad.fr/558646/ http://agritrop.cirad.fr/558646/ A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]. Munoz Bodnar Alejandra, Durand Karine, Vernière Christian, Pruvost Olivier, Jacques Marie Agnès, Verdier Valérie, Koebnik Ralf. 2010. In : Neuvièmes Rencontres plantes-bactéries, 18-22 janvier 2010, Aussois (France) : résumés. SFP, IRD. Paris : SFP, Résumé, 109. Rencontres plantes-bactéries. 9, Aussois, France, 18 Janvier 2010/22 Janvier 2010. Researchers A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] Munoz Bodnar, Alejandra Durand, Karine Vernière, Christian Pruvost, Olivier Jacques, Marie Agnès Verdier, Valérie Koebnik, Ralf eng 2010 SFP Neuvièmes Rencontres plantes-bactéries, 18-22 janvier 2010, Aussois (France) : résumés H20 - Maladies des plantes Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=166616 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H20 - Maladies des plantes H20 - Maladies des plantes |
spellingShingle |
H20 - Maladies des plantes H20 - Maladies des plantes Munoz Bodnar, Alejandra Durand, Karine Vernière, Christian Pruvost, Olivier Jacques, Marie Agnès Verdier, Valérie Koebnik, Ralf A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
description |
Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral) |
format |
conference_item |
topic_facet |
H20 - Maladies des plantes |
author |
Munoz Bodnar, Alejandra Durand, Karine Vernière, Christian Pruvost, Olivier Jacques, Marie Agnès Verdier, Valérie Koebnik, Ralf |
author_facet |
Munoz Bodnar, Alejandra Durand, Karine Vernière, Christian Pruvost, Olivier Jacques, Marie Agnès Verdier, Valérie Koebnik, Ralf |
author_sort |
Munoz Bodnar, Alejandra |
title |
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
title_short |
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
title_full |
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
title_fullStr |
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
title_full_unstemmed |
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] |
title_sort |
novel molecular typing system for plant-pathogenic xanthomonas : [n° 43] |
publisher |
SFP |
url |
http://agritrop.cirad.fr/558646/ |
work_keys_str_mv |
AT munozbodnaralejandra anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT durandkarine anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT vernierechristian anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT pruvostolivier anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT jacquesmarieagnes anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT verdiervalerie anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT koebnikralf anovelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT munozbodnaralejandra novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT durandkarine novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT vernierechristian novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT pruvostolivier novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT jacquesmarieagnes novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT verdiervalerie novelmoleculartypingsystemforplantpathogenicxanthomonasn43 AT koebnikralf novelmoleculartypingsystemforplantpathogenicxanthomonasn43 |
_version_ |
1758022774197059584 |