A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]

A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]

Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral)

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Main Authors: Munoz Bodnar, Alejandra, Durand, Karine, Vernière, Christian, Pruvost, Olivier, Jacques, Marie Agnès, Verdier, Valérie, Koebnik, Ralf
Format: conference_item biblioteca
Language:eng
Published: SFP
Subjects:H20 - Maladies des plantes,
Online Access:http://agritrop.cirad.fr/558646/
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spelling dig-cirad-fr-5586462019-04-12T16:05:49Z http://agritrop.cirad.fr/558646/ http://agritrop.cirad.fr/558646/ A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]. Munoz Bodnar Alejandra, Durand Karine, Vernière Christian, Pruvost Olivier, Jacques Marie Agnès, Verdier Valérie, Koebnik Ralf. 2010. In : Neuvièmes Rencontres plantes-bactéries, 18-22 janvier 2010, Aussois (France) : résumés. SFP, IRD. Paris : SFP, Résumé, 109. Rencontres plantes-bactéries. 9, Aussois, France, 18 Janvier 2010/22 Janvier 2010. Researchers A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43] Munoz Bodnar, Alejandra Durand, Karine Vernière, Christian Pruvost, Olivier Jacques, Marie Agnès Verdier, Valérie Koebnik, Ralf eng 2010 SFP Neuvièmes Rencontres plantes-bactéries, 18-22 janvier 2010, Aussois (France) : résumés H20 - Maladies des plantes Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=166616
institution CIRAD FR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cirad-fr
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language eng
topic H20 - Maladies des plantes
H20 - Maladies des plantes
spellingShingle H20 - Maladies des plantes
H20 - Maladies des plantes
Munoz Bodnar, Alejandra
Durand, Karine
Vernière, Christian
Pruvost, Olivier
Jacques, Marie Agnès
Verdier, Valérie
Koebnik, Ralf
A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
description Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) and CRISPR associated sequences (cas) form hypervariable loci which are widely distributed in prokaryotes. The repetitive region is characterized by short interspersed unique spacer sequences which often match to bacteriophage-derived DNA, thus providing immunity against foreign genetic elements, in particular bacteriophages. Bacteria of the genus Xanthomonas cause diseases on over 400 different host plants, including many economically important crops, such as rice, wheat, citrus, and banana plants. We screened more than 300 strains of Xanthomonas, representing ten different species (44 pathovars), for the presence of CRISPR loci. Only a few pathovars were found to possess a CRISPR locus, among them X. axonopodis pv. vasculorum, X. axonopodis pv. cassavae, X. campestris pv. raphani, X. citri pv. citri, X. oryzae pv. oryzae (Xoo), X. translucens, and X vasicola pv. musacearum. Presence/absence of CRISPR loci appeared to be conserved at the pathovar level, except for Xoo. The apparent absence of CRISPR loci from African Xoo isolates confirms previous results showing that African Xoo isolates form a phylogenetic group that is distant from the Asian Xoo group. Comparative genomics suggested that the common ancestor of all xanthomonads had two CRISPR loci which in most species/pathovars got lost during evolution. Based on DNA sequence information about the terminal spacers of 32 Asian Xoo CRISPR loci we postulate that the common ancestor of these strains had all the spacers which are nowadays still found in a few strains and that some spacers got lost during evolution in some Xoo lineages. This work represents the first proof of concept of CRISPR analysis as a molecular tool for high-resolution strain typing, phylogenetic studies, and global surveillance of a phytopathogen. (Texte intégral)
format conference_item
topic_facet H20 - Maladies des plantes
author Munoz Bodnar, Alejandra
Durand, Karine
Vernière, Christian
Pruvost, Olivier
Jacques, Marie Agnès
Verdier, Valérie
Koebnik, Ralf
author_facet Munoz Bodnar, Alejandra
Durand, Karine
Vernière, Christian
Pruvost, Olivier
Jacques, Marie Agnès
Verdier, Valérie
Koebnik, Ralf
author_sort Munoz Bodnar, Alejandra
title A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
title_short A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
title_full A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
title_fullStr A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
title_full_unstemmed A novel molecular typing system for plant-pathogenic xanthomonas : [N° 43]
title_sort novel molecular typing system for plant-pathogenic xanthomonas : [n° 43]
publisher SFP
url http://agritrop.cirad.fr/558646/
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