Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses
Gossypium australe F. Muell (2n = 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum · G. australe) · G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs.
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Subjects: | F30 - Génétique et amélioration des plantes, Gossypium, Gossypium hirsutum, http://aims.fao.org/aos/agrovoc/c_3335, http://aims.fao.org/aos/agrovoc/c_3339, http://aims.fao.org/aos/agrovoc/c_870, |
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dig-cirad-fr-5583332022-12-30T18:33:47Z http://agritrop.cirad.fr/558333/ http://agritrop.cirad.fr/558333/ Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses. Sarr D., Lacape Jean-Marc, Rodier-Goud Marguerite, Jacquemin Jean-Marie, Benbouza Halima, Toussaint André, Palm Rudy, Ahoton L., Baudoin Jean-Pierre, Mergeai Guy. 2011. Plant Breeding, 130 (1) : 60-66.https://doi.org/10.1111/j.1439-0523.2010.01819.x <https://doi.org/10.1111/j.1439-0523.2010.01819.x> Researchers Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses Sarr, D. Lacape, Jean-Marc Rodier-Goud, Marguerite Jacquemin, Jean-Marie Benbouza, Halima Toussaint, André Palm, Rudy Ahoton, L. Baudoin, Jean-Pierre Mergeai, Guy eng 2011 Plant Breeding F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 Belgique http://aims.fao.org/aos/agrovoc/c_870 Gossypium australe F. Muell (2n = 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum · G. australe) · G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/558333/1/document_558333.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1439-0523.2010.01819.x 10.1111/j.1439-0523.2010.01819.x info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1439-0523.2010.01819.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1439-0523.2010.01819.x |
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F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 http://aims.fao.org/aos/agrovoc/c_870 F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 http://aims.fao.org/aos/agrovoc/c_870 |
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F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 http://aims.fao.org/aos/agrovoc/c_870 F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 http://aims.fao.org/aos/agrovoc/c_870 Sarr, D. Lacape, Jean-Marc Rodier-Goud, Marguerite Jacquemin, Jean-Marie Benbouza, Halima Toussaint, André Palm, Rudy Ahoton, L. Baudoin, Jean-Pierre Mergeai, Guy Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
description |
Gossypium australe F. Muell (2n = 2x = 26) is a wild perennial species possessing agronomic useful traits that would be interesting to introgress into G. hirsutum L. (2n = 4x = 52), the main cultivated cotton species. To isolate monosomic alien addition lines (MAALs) of G. australe in G. hirsutum, the [2(G. hirsutum · G. australe) · G. hirsutum] pentaploid (2n = 5x = 65) was backcrossed as male parent to G. hirsutum. Analysis of 42 BC1 plants and seven alien addition lines, already available, with 150 SSR markers developed from G. hirsutum revealed a cross-species amplification rate of 100% and a polymorphism rate of 56%. Eighty polymorphic SSR markers generated 87 G. australe-specific loci that have been assigned by a hierarchical cluster analysis to 13 linkage groups corresponding to the 13 chromosomes of G. australe. Analysis by SSR markers and genomic in situ hybridization of the self-progeny of disomic alien addition lines, backcross progeny of the pentaploid, allowed the isolation of five new MAALs. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Gossypium Gossypium hirsutum http://aims.fao.org/aos/agrovoc/c_3335 http://aims.fao.org/aos/agrovoc/c_3339 http://aims.fao.org/aos/agrovoc/c_870 |
author |
Sarr, D. Lacape, Jean-Marc Rodier-Goud, Marguerite Jacquemin, Jean-Marie Benbouza, Halima Toussaint, André Palm, Rudy Ahoton, L. Baudoin, Jean-Pierre Mergeai, Guy |
author_facet |
Sarr, D. Lacape, Jean-Marc Rodier-Goud, Marguerite Jacquemin, Jean-Marie Benbouza, Halima Toussaint, André Palm, Rudy Ahoton, L. Baudoin, Jean-Pierre Mergeai, Guy |
author_sort |
Sarr, D. |
title |
Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
title_short |
Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
title_full |
Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
title_fullStr |
Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
title_full_unstemmed |
Isolation of five new monosomic alien addition lines of Gossypium australe F. Muell in G. hirsutum L. by SSR and GISH analyses |
title_sort |
isolation of five new monosomic alien addition lines of gossypium australe f. muell in g. hirsutum l. by ssr and gish analyses |
url |
http://agritrop.cirad.fr/558333/ http://agritrop.cirad.fr/558333/1/document_558333.pdf |
work_keys_str_mv |
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