Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation
We examined the effects of an ectomycorrhizal (EM) fungus, Pisolithus sp., on of the growth of Pinus halepensis (Allepo pine) seedlings, soil microbial functions and rock phosphate solubilization in a un-disinfected soil amended or not with a Moroccan rock phosphate. Allepo pine seedlings were inoculated with an EM fungus (Pisolithus sp. strain PH4) isolated from a P. halepensis plantation and selected for its high ability to mobilize P from an inorganic form of phosphate. After 4 month's culture in a disinfected substrate, plants were transferred in 10 l-containers filled with a natural forest soil and amended or not with rock phosphate powder. After 12 month's culturing, the growth, needle nutrient concentrations of P. halepensis plants were measured. Soil microbial catabolic diversity was assessed by measuring CO2 production of substrate induced respiration responses. Fluorescent pseudomonads were isolated from each soil treatment and tested in axenic conditions for their ability to solubilize a source of inorganic phosphate. The results clearly showed that (i) P. halepensis growth was greatly promoted by the presence of the ectomycorrhizal fungus Pisolithus strain PH4 in a disinfected soil/vermiculite mixture and in a non disinfected soil, (ii) ectomycorrhizal inoculation induced significant changes in the functions of soil microbial communities and selected microorganisms potentially beneficial to the plant growth (i.e. phosphate-solubilizing fluorescent pseudomonad) and (iii) rock phosphate solubilisation was mainly dependent on EM inoculation and mycorrhizosphere microorganisms. These results were in accordance with previous studies where it was demonstrated that EM symbiosis has a beneficial effect on plant growth through a direct effect on the host plant but also an indirect effect via a selective pressure on soil microbiota that favours microorganisms potentially beneficial to plant growth.
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Subjects: | F62 - Physiologie végétale - Croissance et développement, P34 - Biologie du sol, P35 - Fertilité du sol, Pinus halepensis, Pisolithus, http://aims.fao.org/aos/agrovoc/c_5899, http://aims.fao.org/aos/agrovoc/c_23961, http://aims.fao.org/aos/agrovoc/c_4940, |
Online Access: | http://agritrop.cirad.fr/552473/ http://agritrop.cirad.fr/552473/1/document_552473.pdf |
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dig-cirad-fr-5524732022-04-21T07:45:16Z http://agritrop.cirad.fr/552473/ http://agritrop.cirad.fr/552473/ Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation. Ouahmane Lahcen, Revel J.C., Hafidi Mohamed, Thioulouse Jean, Prin Yves, Galiana Antoine, Dreyfus Bernard, Duponnois Robin. 2009. Plant and Soil, 320 (1-2) : 169-179.https://doi.org/10.1007/s11104-008-9882-z <https://doi.org/10.1007/s11104-008-9882-z> Researchers Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation Ouahmane, Lahcen Revel, J.C. Hafidi, Mohamed Thioulouse, Jean Prin, Yves Galiana, Antoine Dreyfus, Bernard Duponnois, Robin eng 2009 Plant and Soil F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 Maroc http://aims.fao.org/aos/agrovoc/c_4940 We examined the effects of an ectomycorrhizal (EM) fungus, Pisolithus sp., on of the growth of Pinus halepensis (Allepo pine) seedlings, soil microbial functions and rock phosphate solubilization in a un-disinfected soil amended or not with a Moroccan rock phosphate. Allepo pine seedlings were inoculated with an EM fungus (Pisolithus sp. strain PH4) isolated from a P. halepensis plantation and selected for its high ability to mobilize P from an inorganic form of phosphate. After 4 month's culture in a disinfected substrate, plants were transferred in 10 l-containers filled with a natural forest soil and amended or not with rock phosphate powder. After 12 month's culturing, the growth, needle nutrient concentrations of P. halepensis plants were measured. Soil microbial catabolic diversity was assessed by measuring CO2 production of substrate induced respiration responses. Fluorescent pseudomonads were isolated from each soil treatment and tested in axenic conditions for their ability to solubilize a source of inorganic phosphate. The results clearly showed that (i) P. halepensis growth was greatly promoted by the presence of the ectomycorrhizal fungus Pisolithus strain PH4 in a disinfected soil/vermiculite mixture and in a non disinfected soil, (ii) ectomycorrhizal inoculation induced significant changes in the functions of soil microbial communities and selected microorganisms potentially beneficial to the plant growth (i.e. phosphate-solubilizing fluorescent pseudomonad) and (iii) rock phosphate solubilisation was mainly dependent on EM inoculation and mycorrhizosphere microorganisms. These results were in accordance with previous studies where it was demonstrated that EM symbiosis has a beneficial effect on plant growth through a direct effect on the host plant but also an indirect effect via a selective pressure on soil microbiota that favours microorganisms potentially beneficial to plant growth. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/552473/1/document_552473.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s11104-008-9882-z 10.1007/s11104-008-9882-z http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=206580 info:eu-repo/semantics/altIdentifier/doi/10.1007/s11104-008-9882-z info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s11104-008-9882-z |
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F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_4940 F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_4940 |
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F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_4940 F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_4940 Ouahmane, Lahcen Revel, J.C. Hafidi, Mohamed Thioulouse, Jean Prin, Yves Galiana, Antoine Dreyfus, Bernard Duponnois, Robin Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
description |
We examined the effects of an ectomycorrhizal (EM) fungus, Pisolithus sp., on of the growth of Pinus halepensis (Allepo pine) seedlings, soil microbial functions and rock phosphate solubilization in a un-disinfected soil amended or not with a Moroccan rock phosphate. Allepo pine seedlings were inoculated with an EM fungus (Pisolithus sp. strain PH4) isolated from a P. halepensis plantation and selected for its high ability to mobilize P from an inorganic form of phosphate. After 4 month's culture in a disinfected substrate, plants were transferred in 10 l-containers filled with a natural forest soil and amended or not with rock phosphate powder. After 12 month's culturing, the growth, needle nutrient concentrations of P. halepensis plants were measured. Soil microbial catabolic diversity was assessed by measuring CO2 production of substrate induced respiration responses. Fluorescent pseudomonads were isolated from each soil treatment and tested in axenic conditions for their ability to solubilize a source of inorganic phosphate. The results clearly showed that (i) P. halepensis growth was greatly promoted by the presence of the ectomycorrhizal fungus Pisolithus strain PH4 in a disinfected soil/vermiculite mixture and in a non disinfected soil, (ii) ectomycorrhizal inoculation induced significant changes in the functions of soil microbial communities and selected microorganisms potentially beneficial to the plant growth (i.e. phosphate-solubilizing fluorescent pseudomonad) and (iii) rock phosphate solubilisation was mainly dependent on EM inoculation and mycorrhizosphere microorganisms. These results were in accordance with previous studies where it was demonstrated that EM symbiosis has a beneficial effect on plant growth through a direct effect on the host plant but also an indirect effect via a selective pressure on soil microbiota that favours microorganisms potentially beneficial to plant growth. |
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F62 - Physiologie végétale - Croissance et développement P34 - Biologie du sol P35 - Fertilité du sol Pinus halepensis Pisolithus http://aims.fao.org/aos/agrovoc/c_5899 http://aims.fao.org/aos/agrovoc/c_23961 http://aims.fao.org/aos/agrovoc/c_4940 |
author |
Ouahmane, Lahcen Revel, J.C. Hafidi, Mohamed Thioulouse, Jean Prin, Yves Galiana, Antoine Dreyfus, Bernard Duponnois, Robin |
author_facet |
Ouahmane, Lahcen Revel, J.C. Hafidi, Mohamed Thioulouse, Jean Prin, Yves Galiana, Antoine Dreyfus, Bernard Duponnois, Robin |
author_sort |
Ouahmane, Lahcen |
title |
Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
title_short |
Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
title_full |
Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
title_fullStr |
Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
title_full_unstemmed |
Responses of Pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
title_sort |
responses of pinus halepensis growth, soil microbial catabolic functions and phosphate-solubilizing bacteria after rock phosphate amendment and ectomycorrhizal inoculation |
url |
http://agritrop.cirad.fr/552473/ http://agritrop.cirad.fr/552473/1/document_552473.pdf |
work_keys_str_mv |
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