Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity
In Barley, the dominant Hooded (K) phenotype is associated with the duplication of a 305 bp element in intron IV of the knox gene BKn3. This regulatory element seems to act as an enhancer and causes ectopic expression of Bkn3 in the lemma-awn transition zone, leading to the formation of a new meristem that develops into an epiphyillic flower. A one hybrid screening aimed at isolating putative regulators of the BKn3 gene uncovered four different proteins capable of interacting with the 305 bp element (K Intron Binding Proteins, KIBPs). In order to gain insight into their role in BKn3 regulation, recombinant KIBPs have been expressed in different prokaryotic systems and the full length proteins have been purified by affinity chromatography. In vitro binding of KIBPs to the 305 bp enhancer has been confirmed by "Electro Mobility Shift Assay" (EMSA) and the minimal binding sites have been assessed. As there is no clear association between KIBP loci and mutations already mapped and characterised in barley, the in vivo function of KIBPs has been investigated in the model species Oryza sativa. Screening of insertional mutant collections has yielded stable lines carrying mutations in two KIBP-related genes: in the first line, a Ds insertion causes defects in tillering and internode elongation, while in the second line, a T -DNA insertion is responsible for lethality in 4 weeks-old seedlings. In addition, morphogenetic effects of KIBPs overexpression in transgenic rice plants are under investigation. (Texte intégral)
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dig-cirad-fr-5423452008-12-09T12:00:00Z http://agritrop.cirad.fr/542345/ http://agritrop.cirad.fr/542345/ Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity. Osnato Michela, Meynard Donaldo, Guiderdoni Emmanuel, Salamini Francesco, Pozzi Carlo, Rossini Laura. 2006. In : Plant Genomics European Meetings, Venice, 2006. Bologne : Ed. Avenue media, Résumé, 250. Plant Genomics European Meetings. 5, Venise, Italie, 11 Octobre 2006/14 Octobre 2006. Researchers Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity Osnato, Michela Meynard, Donaldo Guiderdoni, Emmanuel Salamini, Francesco Pozzi, Carlo Rossini, Laura eng 2006 Ed. Avenue media Plant Genomics European Meetings, Venice, 2006 F30 - Génétique et amélioration des plantes In Barley, the dominant Hooded (K) phenotype is associated with the duplication of a 305 bp element in intron IV of the knox gene BKn3. This regulatory element seems to act as an enhancer and causes ectopic expression of Bkn3 in the lemma-awn transition zone, leading to the formation of a new meristem that develops into an epiphyillic flower. A one hybrid screening aimed at isolating putative regulators of the BKn3 gene uncovered four different proteins capable of interacting with the 305 bp element (K Intron Binding Proteins, KIBPs). In order to gain insight into their role in BKn3 regulation, recombinant KIBPs have been expressed in different prokaryotic systems and the full length proteins have been purified by affinity chromatography. In vitro binding of KIBPs to the 305 bp enhancer has been confirmed by "Electro Mobility Shift Assay" (EMSA) and the minimal binding sites have been assessed. As there is no clear association between KIBP loci and mutations already mapped and characterised in barley, the in vivo function of KIBPs has been investigated in the model species Oryza sativa. Screening of insertional mutant collections has yielded stable lines carrying mutations in two KIBP-related genes: in the first line, a Ds insertion causes defects in tillering and internode elongation, while in the second line, a T -DNA insertion is responsible for lethality in 4 weeks-old seedlings. In addition, morphogenetic effects of KIBPs overexpression in transgenic rice plants are under investigation. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=198358 |
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F30 - Génétique et amélioration des plantes F30 - Génétique et amélioration des plantes Osnato, Michela Meynard, Donaldo Guiderdoni, Emmanuel Salamini, Francesco Pozzi, Carlo Rossini, Laura Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
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In Barley, the dominant Hooded (K) phenotype is associated with the duplication of a 305 bp element in intron IV of the knox gene BKn3. This regulatory element seems to act as an enhancer and causes ectopic expression of Bkn3 in the lemma-awn transition zone, leading to the formation of a new meristem that develops into an epiphyillic flower. A one hybrid screening aimed at isolating putative regulators of the BKn3 gene uncovered four different proteins capable of interacting with the 305 bp element (K Intron Binding Proteins, KIBPs). In order to gain insight into their role in BKn3 regulation, recombinant KIBPs have been expressed in different prokaryotic systems and the full length proteins have been purified by affinity chromatography. In vitro binding of KIBPs to the 305 bp enhancer has been confirmed by "Electro Mobility Shift Assay" (EMSA) and the minimal binding sites have been assessed. As there is no clear association between KIBP loci and mutations already mapped and characterised in barley, the in vivo function of KIBPs has been investigated in the model species Oryza sativa. Screening of insertional mutant collections has yielded stable lines carrying mutations in two KIBP-related genes: in the first line, a Ds insertion causes defects in tillering and internode elongation, while in the second line, a T -DNA insertion is responsible for lethality in 4 weeks-old seedlings. In addition, morphogenetic effects of KIBPs overexpression in transgenic rice plants are under investigation. (Texte intégral) |
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conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes |
author |
Osnato, Michela Meynard, Donaldo Guiderdoni, Emmanuel Salamini, Francesco Pozzi, Carlo Rossini, Laura |
author_facet |
Osnato, Michela Meynard, Donaldo Guiderdoni, Emmanuel Salamini, Francesco Pozzi, Carlo Rossini, Laura |
author_sort |
Osnato, Michela |
title |
Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
title_short |
Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
title_full |
Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
title_fullStr |
Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
title_full_unstemmed |
Characterization of putative regulatores of BKn3, a barley homeobox gene involved in meristematic activity |
title_sort |
characterization of putative regulatores of bkn3, a barley homeobox gene involved in meristematic activity |
publisher |
Ed. Avenue media |
url |
http://agritrop.cirad.fr/542345/ |
work_keys_str_mv |
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