Evaluation of the mitochondrial COI gene as a genetic marker for Myndus crudus and other Myndus spp.

In the Americas and Caribbean, the lethal yellowing (LY) phytoplasma causes a disease of palm transmitted by the leafhopper Myndus crudus (Cixiidae). In Vanuatu and Ghana, foliar decay disease virus affects coconut palm for which M. taffini is a suspected vector. Recently, high mortality in disease-resistant palms has occurred widespread. The availability of a molecular marker would be useful for tracking distribution and dispersal behaviors of Myndus and related vector species in epidemic and non-epidemic areas. We employed PCR and mtCOI primers [F-C1-J-2195, R-L2-N-3014] to amplify a 780bp fragment from insect DNA extracts, and determined the DNA sequence. Phylogenetic analysis revealed three corresponding Myndus clades: adiopodoumeensis (Ghana), crudus (Caribbean), taffinii (Vanuvatu). M. taffini formed two sister-groups. M. crudus formed one group comprising the collection from Oaxaca-MEX, and a second containing FL-USA, Honduras, Jamaica, and Merida-MEX collections. (Texte intégral)

Bibliographic Details

Main Authors:	Brown, J.K., Dollet, Michel
Format:	conference_item biblioteca
Language:	eng
Published:	s.n.
Subjects:	H20 - Maladies des plantes, U30 - Méthodes de recherche,
Online Access:	http://agritrop.cirad.fr/539081/
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