Purification and characterization of cassiicolin, the toxin produced by Corynespora cassiicola, causal agent of the leaf fall disease of rubber tree

Cassiicolin, a phytotoxin produced by the necrotrophic fungus Corynespora cassiicola, was purified to homogeneity from a rubber tree isolate. The optimized protocol involves reverse phase chromatography followed by size exclusion chromatography, with monitoring of the toxicity on detached rubber tree leaves. Cassiicolin appeared to be a peptide composed of 27 amino acids, glycosylated on the second residue, with a N-terminal pyroglutamic acid and 6 cysteines involved in disulfide bonds. Its molecular mass was estimated to he 2885 Da. No significant sequence homology with other proteins could be found. The availability of pure toxin in sufficient amount is a prerequisite for its structure determination, which is a key step in the understanding of the aggression mechanism. © 2006 Published by Elsevier B.V.

Bibliographic Details

Main Authors:	De Lamotte, Frédéric, Duviau, Marie-Pierre, Sanier, Christine, Thai, Robert, Poncet, Joël, Bieysse, Daniel, Breton, Frédéric, Pujade-Renaud, Valérie
Format:	article biblioteca
Language:	eng
Published:	Elsevier
Subjects:	H20 - Maladies des plantes, U30 - Méthodes de recherche, Corynespora cassiicola, toxine, glycoprotéine, purification, chromatographie, séquence nucléotidique, spectrométrie de masse, Hevea brasiliensis, http://aims.fao.org/aos/agrovoc/c_33337, http://aims.fao.org/aos/agrovoc/c_7828, http://aims.fao.org/aos/agrovoc/c_3307, http://aims.fao.org/aos/agrovoc/c_6377, http://aims.fao.org/aos/agrovoc/c_1594, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_12421, http://aims.fao.org/aos/agrovoc/c_3589,
Online Access:	http://agritrop.cirad.fr/538743/ http://agritrop.cirad.fr/538743/1/document_538743.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!