Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads
Here we characterized the effect of the ectomycorrhizal symbiosis on the genotypic and functional diversity of soil Pseudomonas fluorescens populations and analysed its possible consequences in terms of plant nutrition, development and health. Sixty strains of P. fluorescens were isolated from the bulk soil of a forest nursery, the ectomycorrhizosphere and the ectomycorrhizas of the Douglas fir (Pseudostuga menziesii) seedlings-Laccaria bicolor S238N. They were characterized in vitro with the following criteria: ARDRA, phosphate solubilization, siderophore, HCN and AIA production, genes of N2-fixation and antibiotic synthesis, in vitro confrontation with a range of phytopathogenic and ectomycorrhizal fungi, effect on the Douglas fir-L. bicolor symbiosis. For most of these criteria, we demonstrated that the ectomycorrhizosphere significantly structures the P. fluorescens populations and selects strains potentially beneficial to the symbiosis and to the plant. This prompts us to propose the ectomycorrhizal symbiosis as a true microbial complex where multitrophic interactions take place. Moreover it underlines the fact that this symbiosis has an indirect positive effect on plant growth, via its selective pressure on bacterial communities, in addition to its known direct positive effect.
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dig-cirad-fr-5360872024-01-28T15:01:38Z http://agritrop.cirad.fr/536087/ http://agritrop.cirad.fr/536087/ Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads. Frey-Klett Pascale, Chavatte Michaël, Clausse Marie-Lise, Courrier Sébastien, Le Roux Christine, Raaijmakers Jos, Martinotti Maria Giovanna, Pierrat Jean-Claude, Garbaye Jean. 2005. New Phytologist, 165 (1) : 317-328.https://doi.org/10.1111/j.1469-8137.2004.01212.x <https://doi.org/10.1111/j.1469-8137.2004.01212.x> Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads Frey-Klett, Pascale Chavatte, Michaël Clausse, Marie-Lise Courrier, Sébastien Le Roux, Christine Raaijmakers, Jos Martinotti, Maria Giovanna Pierrat, Jean-Claude Garbaye, Jean eng 2005 New Phytologist P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 Here we characterized the effect of the ectomycorrhizal symbiosis on the genotypic and functional diversity of soil Pseudomonas fluorescens populations and analysed its possible consequences in terms of plant nutrition, development and health. Sixty strains of P. fluorescens were isolated from the bulk soil of a forest nursery, the ectomycorrhizosphere and the ectomycorrhizas of the Douglas fir (Pseudostuga menziesii) seedlings-Laccaria bicolor S238N. They were characterized in vitro with the following criteria: ARDRA, phosphate solubilization, siderophore, HCN and AIA production, genes of N2-fixation and antibiotic synthesis, in vitro confrontation with a range of phytopathogenic and ectomycorrhizal fungi, effect on the Douglas fir-L. bicolor symbiosis. For most of these criteria, we demonstrated that the ectomycorrhizosphere significantly structures the P. fluorescens populations and selects strains potentially beneficial to the symbiosis and to the plant. This prompts us to propose the ectomycorrhizal symbiosis as a true microbial complex where multitrophic interactions take place. Moreover it underlines the fact that this symbiosis has an indirect positive effect on plant growth, via its selective pressure on bacterial communities, in addition to its known direct positive effect. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/536087/1/document_536087.pdf application/pdf Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1111/j.1469-8137.2004.01212.x 10.1111/j.1469-8137.2004.01212.x http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=194900 info:eu-repo/semantics/altIdentifier/doi/10.1111/j.1469-8137.2004.01212.x info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1111/j.1469-8137.2004.01212.x |
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P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 |
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P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 Frey-Klett, Pascale Chavatte, Michaël Clausse, Marie-Lise Courrier, Sébastien Le Roux, Christine Raaijmakers, Jos Martinotti, Maria Giovanna Pierrat, Jean-Claude Garbaye, Jean Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
description |
Here we characterized the effect of the ectomycorrhizal symbiosis on the genotypic and functional diversity of soil Pseudomonas fluorescens populations and analysed its possible consequences in terms of plant nutrition, development and health. Sixty strains of P. fluorescens were isolated from the bulk soil of a forest nursery, the ectomycorrhizosphere and the ectomycorrhizas of the Douglas fir (Pseudostuga menziesii) seedlings-Laccaria bicolor S238N. They were characterized in vitro with the following criteria: ARDRA, phosphate solubilization, siderophore, HCN and AIA production, genes of N2-fixation and antibiotic synthesis, in vitro confrontation with a range of phytopathogenic and ectomycorrhizal fungi, effect on the Douglas fir-L. bicolor symbiosis. For most of these criteria, we demonstrated that the ectomycorrhizosphere significantly structures the P. fluorescens populations and selects strains potentially beneficial to the symbiosis and to the plant. This prompts us to propose the ectomycorrhizal symbiosis as a true microbial complex where multitrophic interactions take place. Moreover it underlines the fact that this symbiosis has an indirect positive effect on plant growth, via its selective pressure on bacterial communities, in addition to its known direct positive effect. |
format |
article |
topic_facet |
P34 - Biologie du sol Bacteria Mycorhizé Pseudomonas fluorescens Pseudotsuga menziesii Laccaria symbiose rhizosphère variation génétique dynamique des populations expérimentation in vitro croissance nutrition des plantes santé des plantes ectomycorhize http://aims.fao.org/aos/agrovoc/c_765 http://aims.fao.org/aos/agrovoc/c_5023 http://aims.fao.org/aos/agrovoc/c_26647 http://aims.fao.org/aos/agrovoc/c_6312 http://aims.fao.org/aos/agrovoc/c_31710 http://aims.fao.org/aos/agrovoc/c_7563 http://aims.fao.org/aos/agrovoc/c_6569 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6111 http://aims.fao.org/aos/agrovoc/c_16131 http://aims.fao.org/aos/agrovoc/c_3394 http://aims.fao.org/aos/agrovoc/c_16379 http://aims.fao.org/aos/agrovoc/c_25398 http://aims.fao.org/aos/agrovoc/c_35482 |
author |
Frey-Klett, Pascale Chavatte, Michaël Clausse, Marie-Lise Courrier, Sébastien Le Roux, Christine Raaijmakers, Jos Martinotti, Maria Giovanna Pierrat, Jean-Claude Garbaye, Jean |
author_facet |
Frey-Klett, Pascale Chavatte, Michaël Clausse, Marie-Lise Courrier, Sébastien Le Roux, Christine Raaijmakers, Jos Martinotti, Maria Giovanna Pierrat, Jean-Claude Garbaye, Jean |
author_sort |
Frey-Klett, Pascale |
title |
Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
title_short |
Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
title_full |
Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
title_fullStr |
Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
title_full_unstemmed |
Ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
title_sort |
ectomycorrhizal symbiosis affects functional diversity of rhizosphere fluorescent pseudomonads |
url |
http://agritrop.cirad.fr/536087/ http://agritrop.cirad.fr/536087/1/document_536087.pdf |
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