Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island
Yellow leaf syndrome of sugarcane caused by the Sugarcane yellow leaf virus (SCYLV) was first reported in Hawaii and in Brazil in the late 1980s and early 1990s. The pathogen was subsequently found to be widespread in most sugarcane producing countries. SCYLV was first detected in Reunion in 1997, but sugarcane leaf yellowing had been observed on the island since 1980. The genetic variability of SCYLV was analysed using 48 virus isolates from Reunion Island and 24 isolates from other countries. One fragment from each of the six ORFs in the genome was amplified by RT-PCR, cloned and then sequenced. Phylogenetic analysis of the obtained sequences showed that the most variable regions of SCYLV are in ORF 1 which codes for a multifunctional protein and ORF 2 which codes for the replicase. On the other hand, the region the most conserved is ORF 3 which codes for the capsid protein. Depending on the region observed, the virus isolates were classified into two to four phylogenetic groups. Based on the variability of the entire genome, the majority of isolates from Reunion Island were different from the isolates of other origins. Four isolates from Reunion Island were, however, very close to these foreign isolates. This suggests that SCYLV was introduced to Reunion Island from another country and that one particular genotype evolved and spread on the island. (Texte intégral)
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Subjects: | H20 - Maladies des plantes, Saccharum, virus des végétaux, variation génétique, http://aims.fao.org/aos/agrovoc/c_6725, http://aims.fao.org/aos/agrovoc/c_5985, http://aims.fao.org/aos/agrovoc/c_15975, http://aims.fao.org/aos/agrovoc/c_6543, http://aims.fao.org/aos/agrovoc/c_3081, |
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dig-cirad-fr-5196192024-01-28T12:23:09Z http://agritrop.cirad.fr/519619/ http://agritrop.cirad.fr/519619/ Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island. Rassaby Laurence, Royer Monique, Borg Zara, Braithwaite Kathryn S., Smith Grant R., Perrier Xavier, Rott Philippe. 2003. In : Solving problems in the real world : ICPP 2003, 8th International Congress of Plant Pathology, 2-7 February 2003, Christchurch, New-Zealand. ISPP. s.l. : s.n., Résumé, 1 p. International Congress of Plant Pathology. 8, Christchurch, Nouvelle-Zélande, 2 Février 2003/7 Février 2003. Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island Rassaby, Laurence Royer, Monique Borg, Zara Braithwaite, Kathryn S. Smith, Grant R. Perrier, Xavier Rott, Philippe eng 2003 s.n. Solving problems in the real world : ICPP 2003, 8th International Congress of Plant Pathology, 2-7 February 2003, Christchurch, New-Zealand H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 La Réunion France http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Yellow leaf syndrome of sugarcane caused by the Sugarcane yellow leaf virus (SCYLV) was first reported in Hawaii and in Brazil in the late 1980s and early 1990s. The pathogen was subsequently found to be widespread in most sugarcane producing countries. SCYLV was first detected in Reunion in 1997, but sugarcane leaf yellowing had been observed on the island since 1980. The genetic variability of SCYLV was analysed using 48 virus isolates from Reunion Island and 24 isolates from other countries. One fragment from each of the six ORFs in the genome was amplified by RT-PCR, cloned and then sequenced. Phylogenetic analysis of the obtained sequences showed that the most variable regions of SCYLV are in ORF 1 which codes for a multifunctional protein and ORF 2 which codes for the replicase. On the other hand, the region the most conserved is ORF 3 which codes for the capsid protein. Depending on the region observed, the virus isolates were classified into two to four phylogenetic groups. Based on the variability of the entire genome, the majority of isolates from Reunion Island were different from the isolates of other origins. Four isolates from Reunion Island were, however, very close to these foreign isolates. This suggests that SCYLV was introduced to Reunion Island from another country and that one particular genotype evolved and spread on the island. (Texte intégral) conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=180896 http://agritrop.cirad.fr/519621/ |
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H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
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H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 Rassaby, Laurence Royer, Monique Borg, Zara Braithwaite, Kathryn S. Smith, Grant R. Perrier, Xavier Rott, Philippe Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
description |
Yellow leaf syndrome of sugarcane caused by the Sugarcane yellow leaf virus (SCYLV) was first reported in Hawaii and in Brazil in the late 1980s and early 1990s. The pathogen was subsequently found to be widespread in most sugarcane producing countries. SCYLV was first detected in Reunion in 1997, but sugarcane leaf yellowing had been observed on the island since 1980. The genetic variability of SCYLV was analysed using 48 virus isolates from Reunion Island and 24 isolates from other countries. One fragment from each of the six ORFs in the genome was amplified by RT-PCR, cloned and then sequenced. Phylogenetic analysis of the obtained sequences showed that the most variable regions of SCYLV are in ORF 1 which codes for a multifunctional protein and ORF 2 which codes for the replicase. On the other hand, the region the most conserved is ORF 3 which codes for the capsid protein. Depending on the region observed, the virus isolates were classified into two to four phylogenetic groups. Based on the variability of the entire genome, the majority of isolates from Reunion Island were different from the isolates of other origins. Four isolates from Reunion Island were, however, very close to these foreign isolates. This suggests that SCYLV was introduced to Reunion Island from another country and that one particular genotype evolved and spread on the island. (Texte intégral) |
format |
conference_item |
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H20 - Maladies des plantes Saccharum virus des végétaux variation génétique http://aims.fao.org/aos/agrovoc/c_6725 http://aims.fao.org/aos/agrovoc/c_5985 http://aims.fao.org/aos/agrovoc/c_15975 http://aims.fao.org/aos/agrovoc/c_6543 http://aims.fao.org/aos/agrovoc/c_3081 |
author |
Rassaby, Laurence Royer, Monique Borg, Zara Braithwaite, Kathryn S. Smith, Grant R. Perrier, Xavier Rott, Philippe |
author_facet |
Rassaby, Laurence Royer, Monique Borg, Zara Braithwaite, Kathryn S. Smith, Grant R. Perrier, Xavier Rott, Philippe |
author_sort |
Rassaby, Laurence |
title |
Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
title_short |
Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
title_full |
Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
title_fullStr |
Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
title_full_unstemmed |
Genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from Reunion Island |
title_sort |
genetic diversity of sugarcane yellow leaf virus isolates from several countries and especially from reunion island |
publisher |
s.n. |
url |
http://agritrop.cirad.fr/519619/ |
work_keys_str_mv |
AT rassabylaurence geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT royermonique geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT borgzara geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT braithwaitekathryns geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT smithgrantr geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT perrierxavier geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland AT rottphilippe geneticdiversityofsugarcaneyellowleafvirusisolatesfromseveralcountriesandespeciallyfromreunionisland |
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