Host specificity of mollicutes oriC plasmids : functional analysis of replication origin
Recently, artificial oriC plasmids containing the chromosomal dnaA gene and surrounding DnaA box sequences were obtained for the mollicutes Spiroplasma citri and Mycoplasma pulmonis. In order to study the specificity of these plasmids among mollicutes, a set of similar oriC plasmids was developed for three mycoplasmas belonging to the mycoides cluster, Mycoplasma mycoides subsp. mycoides LC (MmmLC), M. mycoides subsp. mycoides SC (MmmSC) and Mycoplasma capricolum, subsp. capricolum. Mycoplasmas from the mycoides cluster, S. citri and M. pulmonis were used as recipients for transformation experiments by homologous and heterologous oriC plasmids. All five mollicutes were successfully transformed by homologous plasmids, suggesting that the dnaA gene region represents the functional replication origin of the mollicute chromosomes. However, the ability of mollicutes to replicate heterologous oriC plasmids was found to vary noticeably with the species. For example, the oriC plasmid from M. capricolum did not replicate in the closely related species MmmSC and MmmLC. In contrast, plasmids harbouring the oriC from MmmSC, MmmLC and the more distant species S. citri were all found to replicate in M. capricolum. Our results suggest that the cis-elements present in oriC sequences are not the only determinants of this host specificity.
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Subjects: | L50 - Physiologie et biochimie animales, plasmide, séquence nucléotidique, Mycoplasma pulmonis, Mycoplasma mycoides, http://aims.fao.org/aos/agrovoc/c_16014, http://aims.fao.org/aos/agrovoc/c_27583, http://aims.fao.org/aos/agrovoc/c_27250, http://aims.fao.org/aos/agrovoc/c_23951, |
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dig-cirad-fr-5171922024-01-28T11:59:19Z http://agritrop.cirad.fr/517192/ http://agritrop.cirad.fr/517192/ Host specificity of mollicutes oriC plasmids : functional analysis of replication origin. Lartigue Carole, Renaudin Joël, Blanchard Alain, Thiaucourt François, Sirand-Pugnet Pascal. 2003. Nucleic Acids Research, 31 (22) : 6610-6618.https://doi.org/10.1093/nar/gkg848 <https://doi.org/10.1093/nar/gkg848> Host specificity of mollicutes oriC plasmids : functional analysis of replication origin Lartigue, Carole Renaudin, Joël Blanchard, Alain Thiaucourt, François Sirand-Pugnet, Pascal eng 2003 Nucleic Acids Research L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 Recently, artificial oriC plasmids containing the chromosomal dnaA gene and surrounding DnaA box sequences were obtained for the mollicutes Spiroplasma citri and Mycoplasma pulmonis. In order to study the specificity of these plasmids among mollicutes, a set of similar oriC plasmids was developed for three mycoplasmas belonging to the mycoides cluster, Mycoplasma mycoides subsp. mycoides LC (MmmLC), M. mycoides subsp. mycoides SC (MmmSC) and Mycoplasma capricolum, subsp. capricolum. Mycoplasmas from the mycoides cluster, S. citri and M. pulmonis were used as recipients for transformation experiments by homologous and heterologous oriC plasmids. All five mollicutes were successfully transformed by homologous plasmids, suggesting that the dnaA gene region represents the functional replication origin of the mollicute chromosomes. However, the ability of mollicutes to replicate heterologous oriC plasmids was found to vary noticeably with the species. For example, the oriC plasmid from M. capricolum did not replicate in the closely related species MmmSC and MmmLC. In contrast, plasmids harbouring the oriC from MmmSC, MmmLC and the more distant species S. citri were all found to replicate in M. capricolum. Our results suggest that the cis-elements present in oriC sequences are not the only determinants of this host specificity. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/517192/1/517192.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1093/nar/gkg848 10.1093/nar/gkg848 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=179170 info:eu-repo/semantics/altIdentifier/doi/10.1093/nar/gkg848 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/nar/gkg848 |
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L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 |
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L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 Lartigue, Carole Renaudin, Joël Blanchard, Alain Thiaucourt, François Sirand-Pugnet, Pascal Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
description |
Recently, artificial oriC plasmids containing the chromosomal dnaA gene and surrounding DnaA box sequences were obtained for the mollicutes Spiroplasma citri and Mycoplasma pulmonis. In order to study the specificity of these plasmids among mollicutes, a set of similar oriC plasmids was developed for three mycoplasmas belonging to the mycoides cluster, Mycoplasma mycoides subsp. mycoides LC (MmmLC), M. mycoides subsp. mycoides SC (MmmSC) and Mycoplasma capricolum, subsp. capricolum. Mycoplasmas from the mycoides cluster, S. citri and M. pulmonis were used as recipients for transformation experiments by homologous and heterologous oriC plasmids. All five mollicutes were successfully transformed by homologous plasmids, suggesting that the dnaA gene region represents the functional replication origin of the mollicute chromosomes. However, the ability of mollicutes to replicate heterologous oriC plasmids was found to vary noticeably with the species. For example, the oriC plasmid from M. capricolum did not replicate in the closely related species MmmSC and MmmLC. In contrast, plasmids harbouring the oriC from MmmSC, MmmLC and the more distant species S. citri were all found to replicate in M. capricolum. Our results suggest that the cis-elements present in oriC sequences are not the only determinants of this host specificity. |
format |
article |
topic_facet |
L50 - Physiologie et biochimie animales plasmide séquence nucléotidique Mycoplasma pulmonis Mycoplasma mycoides http://aims.fao.org/aos/agrovoc/c_16014 http://aims.fao.org/aos/agrovoc/c_27583 http://aims.fao.org/aos/agrovoc/c_27250 http://aims.fao.org/aos/agrovoc/c_23951 |
author |
Lartigue, Carole Renaudin, Joël Blanchard, Alain Thiaucourt, François Sirand-Pugnet, Pascal |
author_facet |
Lartigue, Carole Renaudin, Joël Blanchard, Alain Thiaucourt, François Sirand-Pugnet, Pascal |
author_sort |
Lartigue, Carole |
title |
Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
title_short |
Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
title_full |
Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
title_fullStr |
Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
title_full_unstemmed |
Host specificity of mollicutes oriC plasmids : functional analysis of replication origin |
title_sort |
host specificity of mollicutes oric plasmids : functional analysis of replication origin |
url |
http://agritrop.cirad.fr/517192/ http://agritrop.cirad.fr/517192/1/517192.pdf |
work_keys_str_mv |
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_version_ |
1792495716522786816 |