Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)

A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points.

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Bibliographic Details
Main Authors: Vilarinhos, Alberto Duarte, Piffanelli, Pietro, Lagoda, Pierre, Thibivilliers, Sandra, Sabau, Xavier, Carreel, Françoise, D'Hont, Angélique
Format: article biblioteca
Language:eng
Published: Springer
Subjects:F30 - Génétique et amélioration des plantes, banque de gènes, Musa acuminata, Musa balbisiana, méthode, adn, http://aims.fao.org/aos/agrovoc/c_11116, http://aims.fao.org/aos/agrovoc/c_4994, http://aims.fao.org/aos/agrovoc/c_4995, http://aims.fao.org/aos/agrovoc/c_4788, http://aims.fao.org/aos/agrovoc/c_2347,
Online Access:http://agritrop.cirad.fr/513207/
http://agritrop.cirad.fr/513207/1/513207.pdf
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spelling dig-cirad-fr-5132072024-12-18T13:04:18Z http://agritrop.cirad.fr/513207/ http://agritrop.cirad.fr/513207/ Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla). Vilarinhos Alberto Duarte, Piffanelli Pietro, Lagoda Pierre, Thibivilliers Sandra, Sabau Xavier, Carreel Françoise, D'Hont Angélique. 2003. Theoretical and Applied Genetics, 106 (6) : 1102-1106.https://doi.org/10.1007/s00122-002-1155-2 <https://doi.org/10.1007/s00122-002-1155-2> Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla) Vilarinhos, Alberto Duarte Piffanelli, Pietro Lagoda, Pierre Thibivilliers, Sandra Sabau, Xavier Carreel, Françoise D'Hont, Angélique eng 2003 Springer Theoretical and Applied Genetics F30 - Génétique et amélioration des plantes banque de gènes Musa acuminata Musa balbisiana méthode adn http://aims.fao.org/aos/agrovoc/c_11116 http://aims.fao.org/aos/agrovoc/c_4994 http://aims.fao.org/aos/agrovoc/c_4995 http://aims.fao.org/aos/agrovoc/c_4788 http://aims.fao.org/aos/agrovoc/c_2347 A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/513207/1/513207.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00122-002-1155-2 10.1007/s00122-002-1155-2 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00122-002-1155-2 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00122-002-1155-2
institution CIRAD FR
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-cirad-fr
tag biblioteca
region Europa del Oeste
libraryname Biblioteca del CIRAD Francia
language eng
topic F30 - Génétique et amélioration des plantes
banque de gènes
Musa acuminata
Musa balbisiana
méthode
adn
http://aims.fao.org/aos/agrovoc/c_11116
http://aims.fao.org/aos/agrovoc/c_4994
http://aims.fao.org/aos/agrovoc/c_4995
http://aims.fao.org/aos/agrovoc/c_4788
http://aims.fao.org/aos/agrovoc/c_2347
F30 - Génétique et amélioration des plantes
banque de gènes
Musa acuminata
Musa balbisiana
méthode
adn
http://aims.fao.org/aos/agrovoc/c_11116
http://aims.fao.org/aos/agrovoc/c_4994
http://aims.fao.org/aos/agrovoc/c_4995
http://aims.fao.org/aos/agrovoc/c_4788
http://aims.fao.org/aos/agrovoc/c_2347
spellingShingle F30 - Génétique et amélioration des plantes
banque de gènes
Musa acuminata
Musa balbisiana
méthode
adn
http://aims.fao.org/aos/agrovoc/c_11116
http://aims.fao.org/aos/agrovoc/c_4994
http://aims.fao.org/aos/agrovoc/c_4995
http://aims.fao.org/aos/agrovoc/c_4788
http://aims.fao.org/aos/agrovoc/c_2347
F30 - Génétique et amélioration des plantes
banque de gènes
Musa acuminata
Musa balbisiana
méthode
adn
http://aims.fao.org/aos/agrovoc/c_11116
http://aims.fao.org/aos/agrovoc/c_4994
http://aims.fao.org/aos/agrovoc/c_4995
http://aims.fao.org/aos/agrovoc/c_4788
http://aims.fao.org/aos/agrovoc/c_2347
Vilarinhos, Alberto Duarte
Piffanelli, Pietro
Lagoda, Pierre
Thibivilliers, Sandra
Sabau, Xavier
Carreel, Françoise
D'Hont, Angélique
Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
description A bacterial artificial chromosome (BAC) library for banana was constructed from leaves of the wild diploid 'Calcutta 4' clone (Musa acuminata subsp. Burmannicoides 2n = 2x = 22). 'Calcutta 4' is widely used in breeding programs for its resistance to the current major disease of banana and is being used to build a genetic reference map of banana. As banana leaves are particularly rich in polyphenols and polysaccharides a protocol was adapted to isolate intact nuclei and high-molecular-weight (HMW) DNA. A total of 55,152 clones with an average insert size of 100 kb were picked. The frequency of BAC clones carrying inserts derived from chloroplast and mitochondrial DNA was estimated to be 1.5%. The coverage of the library is equivalent to 9.0-times the haploid genome. The BAC library was screened with 13 RFLP probes belonging to the 8 linkage groups of the consensus molecular map of banana. A total of 135 clones were identified giving an average of 10.38 clones for each locus. This BAC library will be a valuable starting tool for many of the goals of the recently emerged International Musa Genomic Consortium. One of our initial objectives will be to develop a banana physical map by BAC-FISH (fluorescent in situ hybridization) viewing the characterization of translocation break points.
format article
topic_facet F30 - Génétique et amélioration des plantes
banque de gènes
Musa acuminata
Musa balbisiana
méthode
adn
http://aims.fao.org/aos/agrovoc/c_11116
http://aims.fao.org/aos/agrovoc/c_4994
http://aims.fao.org/aos/agrovoc/c_4995
http://aims.fao.org/aos/agrovoc/c_4788
http://aims.fao.org/aos/agrovoc/c_2347
author Vilarinhos, Alberto Duarte
Piffanelli, Pietro
Lagoda, Pierre
Thibivilliers, Sandra
Sabau, Xavier
Carreel, Françoise
D'Hont, Angélique
author_facet Vilarinhos, Alberto Duarte
Piffanelli, Pietro
Lagoda, Pierre
Thibivilliers, Sandra
Sabau, Xavier
Carreel, Françoise
D'Hont, Angélique
author_sort Vilarinhos, Alberto Duarte
title Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
title_short Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
title_full Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
title_fullStr Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
title_full_unstemmed Construction and characterization of a bacterial artificial chromosome library of banana (Musa acuminata Colla)
title_sort construction and characterization of a bacterial artificial chromosome library of banana (musa acuminata colla)
publisher Springer
url http://agritrop.cirad.fr/513207/
http://agritrop.cirad.fr/513207/1/513207.pdf
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