Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes
This study was aimed at determining whether gene cassettes (promoter-coding sequence-terminator) can be efficiently used in microprojectile acceleration-mediated co-transformation of rice in the place of whole plasmids, and to what extent their use influences the integration and expression of the co-transferred gene of interest. Two non-linked marker genes (yfp and hph) were co-introduced by microprojectile bombardment into cells of embryogenic calli in three separate experiments. Three different DNA structures were compared for their ability to transiently and stably transform rice cells: supercoiled or linearized whole-plasmid DNA, gene cassette DNA and single-stranded gene cassette DNA coated with Escherichia coli single-stranded binding (SSB) proteins. Our results demonstrate that microprojectile bombardment-mediated transformation of rice using gene cassettes is possible without significantly reducing transformation efficiency in comparison to the use of whole-plasmid DNA. Furthermore, no obvious difference in transgene integration pattern and inheritance was observed among plants transformed with gene cassettes compared to those transformed with the whole plasmid, except that concatemerization of molecules prior to integration was rarely observed in gene cassette transformants.
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Subjects: | F30 - Génétique et amélioration des plantes, Oryza sativa, http://aims.fao.org/aos/agrovoc/c_5438, |
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dig-cirad-fr-4908192023-06-29T08:52:42Z http://agritrop.cirad.fr/490819/ http://agritrop.cirad.fr/490819/ Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes. Breitler Jean-Christophe, Labeyrie Axel, Meynard Donaldo, Legavre Thierry, Guiderdoni Emmanuel. 2002. Theoretical and Applied Genetics, 104 (4) : 709-719.https://doi.org/10.1007/s00122-001-0786-z <https://doi.org/10.1007/s00122-001-0786-z> Researchers Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes Breitler, Jean-Christophe Labeyrie, Axel Meynard, Donaldo Legavre, Thierry Guiderdoni, Emmanuel eng 2002 Theoretical and Applied Genetics F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 This study was aimed at determining whether gene cassettes (promoter-coding sequence-terminator) can be efficiently used in microprojectile acceleration-mediated co-transformation of rice in the place of whole plasmids, and to what extent their use influences the integration and expression of the co-transferred gene of interest. Two non-linked marker genes (yfp and hph) were co-introduced by microprojectile bombardment into cells of embryogenic calli in three separate experiments. Three different DNA structures were compared for their ability to transiently and stably transform rice cells: supercoiled or linearized whole-plasmid DNA, gene cassette DNA and single-stranded gene cassette DNA coated with Escherichia coli single-stranded binding (SSB) proteins. Our results demonstrate that microprojectile bombardment-mediated transformation of rice using gene cassettes is possible without significantly reducing transformation efficiency in comparison to the use of whole-plasmid DNA. Furthermore, no obvious difference in transgene integration pattern and inheritance was observed among plants transformed with gene cassettes compared to those transformed with the whole plasmid, except that concatemerization of molecules prior to integration was rarely observed in gene cassette transformants. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/490819/1/490819.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1007/s00122-001-0786-z 10.1007/s00122-001-0786-z http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=172710 info:eu-repo/semantics/altIdentifier/doi/10.1007/s00122-001-0786-z info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1007/s00122-001-0786-z |
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F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 |
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F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 Breitler, Jean-Christophe Labeyrie, Axel Meynard, Donaldo Legavre, Thierry Guiderdoni, Emmanuel Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
description |
This study was aimed at determining whether gene cassettes (promoter-coding sequence-terminator) can be efficiently used in microprojectile acceleration-mediated co-transformation of rice in the place of whole plasmids, and to what extent their use influences the integration and expression of the co-transferred gene of interest. Two non-linked marker genes (yfp and hph) were co-introduced by microprojectile bombardment into cells of embryogenic calli in three separate experiments. Three different DNA structures were compared for their ability to transiently and stably transform rice cells: supercoiled or linearized whole-plasmid DNA, gene cassette DNA and single-stranded gene cassette DNA coated with Escherichia coli single-stranded binding (SSB) proteins. Our results demonstrate that microprojectile bombardment-mediated transformation of rice using gene cassettes is possible without significantly reducing transformation efficiency in comparison to the use of whole-plasmid DNA. Furthermore, no obvious difference in transgene integration pattern and inheritance was observed among plants transformed with gene cassettes compared to those transformed with the whole plasmid, except that concatemerization of molecules prior to integration was rarely observed in gene cassette transformants. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes Oryza sativa http://aims.fao.org/aos/agrovoc/c_5438 |
author |
Breitler, Jean-Christophe Labeyrie, Axel Meynard, Donaldo Legavre, Thierry Guiderdoni, Emmanuel |
author_facet |
Breitler, Jean-Christophe Labeyrie, Axel Meynard, Donaldo Legavre, Thierry Guiderdoni, Emmanuel |
author_sort |
Breitler, Jean-Christophe |
title |
Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
title_short |
Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
title_full |
Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
title_fullStr |
Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
title_full_unstemmed |
Efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
title_sort |
efficient microprojectile bombardment-mediated transformation of rice using gene cassettes |
url |
http://agritrop.cirad.fr/490819/ http://agritrop.cirad.fr/490819/1/490819.pdf |
work_keys_str_mv |
AT breitlerjeanchristophe efficientmicroprojectilebombardmentmediatedtransformationofriceusinggenecassettes AT labeyrieaxel efficientmicroprojectilebombardmentmediatedtransformationofriceusinggenecassettes AT meynarddonaldo efficientmicroprojectilebombardmentmediatedtransformationofriceusinggenecassettes AT legavrethierry efficientmicroprojectilebombardmentmediatedtransformationofriceusinggenecassettes AT guiderdoniemmanuel efficientmicroprojectilebombardmentmediatedtransformationofriceusinggenecassettes |
_version_ |
1770192416900382720 |