Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events
From differential display studies performed on oil palm (Elaeis guineensis Jacq.) tissue cultures bearing or lacking an epigenetic homeotic flowering abnormality, known as mantled, EGAD1, a gene coding for a putative plant defensin, has been identified and characterized. In whole plants, transcripts of the EGAD1 gene were detected only in inflorescences. The closest characterized relative of the oil palm EGAD1 gene is the Petunia PPT gene, which is expressed principally in the pistil of the flower. The 77 amino acid polypeptide encoded by the EGAD1 gene displays strong similarities with a number of plant defensin proteins, which are thought to play a protective role and which have been shown in some cases to possess antifungal properties. Oil palm tissue cultures exhibit a generally strong induction of accumulation of EGAD1 transcripts, which were detected to differing extents at all stages of the tissue culture regeneration process. The 5' flanking region of the EGAD1 gene was found to contain two different types of potential cis-acting DNA element previously identified in the promoters of plant defence-related genes, which may explain the observed expression in tissue cultures. At the callus stage of the in vitro regeneration procedure, a differential accumulation of EGAD1 transcripts was observed which correlated with the presence or absence of the mantled flowering abnormality. EGAD1 gene expression may therefore be a marker of epigenetic somaclonal variation events.
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Subjects: | F30 - Génétique et amélioration des plantes, F60 - Physiologie et biochimie végétale, Elaeis guineensis, variation somaclonale, inflorescence, trouble génétique, expression des gènes, mécanisme de défense, http://aims.fao.org/aos/agrovoc/c_2509, http://aims.fao.org/aos/agrovoc/c_34127, http://aims.fao.org/aos/agrovoc/c_3858, http://aims.fao.org/aos/agrovoc/c_3217, http://aims.fao.org/aos/agrovoc/c_27527, http://aims.fao.org/aos/agrovoc/c_35269, |
Online Access: | http://agritrop.cirad.fr/490761/ http://agritrop.cirad.fr/490761/1/490761.pdf |
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dig-cirad-fr-4907612024-01-28T10:25:08Z http://agritrop.cirad.fr/490761/ http://agritrop.cirad.fr/490761/ Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events. Tregear James, Morcillo Fabienne, Richaud Frédérique, Berger Angélique, Singh Rajinder, Cheah Suan-Choo, Hartmann Caroline, Rival Alain, Duval Yves. 2002. Journal of Experimental Botany, 53 (373) : 1387-1396.https://doi.org/10.1093/jexbot/53.373.1387 <https://doi.org/10.1093/jexbot/53.373.1387> Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events Tregear, James Morcillo, Fabienne Richaud, Frédérique Berger, Angélique Singh, Rajinder Cheah, Suan-Choo Hartmann, Caroline Rival, Alain Duval, Yves eng 2002 Journal of Experimental Botany F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 From differential display studies performed on oil palm (Elaeis guineensis Jacq.) tissue cultures bearing or lacking an epigenetic homeotic flowering abnormality, known as mantled, EGAD1, a gene coding for a putative plant defensin, has been identified and characterized. In whole plants, transcripts of the EGAD1 gene were detected only in inflorescences. The closest characterized relative of the oil palm EGAD1 gene is the Petunia PPT gene, which is expressed principally in the pistil of the flower. The 77 amino acid polypeptide encoded by the EGAD1 gene displays strong similarities with a number of plant defensin proteins, which are thought to play a protective role and which have been shown in some cases to possess antifungal properties. Oil palm tissue cultures exhibit a generally strong induction of accumulation of EGAD1 transcripts, which were detected to differing extents at all stages of the tissue culture regeneration process. The 5' flanking region of the EGAD1 gene was found to contain two different types of potential cis-acting DNA element previously identified in the promoters of plant defence-related genes, which may explain the observed expression in tissue cultures. At the callus stage of the in vitro regeneration procedure, a differential accumulation of EGAD1 transcripts was observed which correlated with the presence or absence of the mantled flowering abnormality. EGAD1 gene expression may therefore be a marker of epigenetic somaclonal variation events. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/490761/1/490761.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1093/jexbot/53.373.1387 10.1093/jexbot/53.373.1387 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=172522 info:eu-repo/semantics/altIdentifier/doi/10.1093/jexbot/53.373.1387 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1093/jexbot/53.373.1387 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 |
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F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 Tregear, James Morcillo, Fabienne Richaud, Frédérique Berger, Angélique Singh, Rajinder Cheah, Suan-Choo Hartmann, Caroline Rival, Alain Duval, Yves Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
description |
From differential display studies performed on oil palm (Elaeis guineensis Jacq.) tissue cultures bearing or lacking an epigenetic homeotic flowering abnormality, known as mantled, EGAD1, a gene coding for a putative plant defensin, has been identified and characterized. In whole plants, transcripts of the EGAD1 gene were detected only in inflorescences. The closest characterized relative of the oil palm EGAD1 gene is the Petunia PPT gene, which is expressed principally in the pistil of the flower. The 77 amino acid polypeptide encoded by the EGAD1 gene displays strong similarities with a number of plant defensin proteins, which are thought to play a protective role and which have been shown in some cases to possess antifungal properties. Oil palm tissue cultures exhibit a generally strong induction of accumulation of EGAD1 transcripts, which were detected to differing extents at all stages of the tissue culture regeneration process. The 5' flanking region of the EGAD1 gene was found to contain two different types of potential cis-acting DNA element previously identified in the promoters of plant defence-related genes, which may explain the observed expression in tissue cultures. At the callus stage of the in vitro regeneration procedure, a differential accumulation of EGAD1 transcripts was observed which correlated with the presence or absence of the mantled flowering abnormality. EGAD1 gene expression may therefore be a marker of epigenetic somaclonal variation events. |
format |
article |
topic_facet |
F30 - Génétique et amélioration des plantes F60 - Physiologie et biochimie végétale Elaeis guineensis variation somaclonale inflorescence trouble génétique expression des gènes mécanisme de défense http://aims.fao.org/aos/agrovoc/c_2509 http://aims.fao.org/aos/agrovoc/c_34127 http://aims.fao.org/aos/agrovoc/c_3858 http://aims.fao.org/aos/agrovoc/c_3217 http://aims.fao.org/aos/agrovoc/c_27527 http://aims.fao.org/aos/agrovoc/c_35269 |
author |
Tregear, James Morcillo, Fabienne Richaud, Frédérique Berger, Angélique Singh, Rajinder Cheah, Suan-Choo Hartmann, Caroline Rival, Alain Duval, Yves |
author_facet |
Tregear, James Morcillo, Fabienne Richaud, Frédérique Berger, Angélique Singh, Rajinder Cheah, Suan-Choo Hartmann, Caroline Rival, Alain Duval, Yves |
author_sort |
Tregear, James |
title |
Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
title_short |
Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
title_full |
Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
title_fullStr |
Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
title_full_unstemmed |
Characterization of a defensin gene expressed in oil palm inflorescences : Induction during tissue culture and possible association with epigenetic somaclonal variation events |
title_sort |
characterization of a defensin gene expressed in oil palm inflorescences : induction during tissue culture and possible association with epigenetic somaclonal variation events |
url |
http://agritrop.cirad.fr/490761/ http://agritrop.cirad.fr/490761/1/490761.pdf |
work_keys_str_mv |
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