Temporary immersion for plant tissue culture
The use of temporary immersion in a liquid medium has been shown to improve plant micropropagation both for quality and economical aspects. The efficiency of the system originates in the improvement of nutrionnal and gazeous exchanges. Periods of immersion should be very brief and were easily controlled by a quick displacement of liquid through the application of an overpressure. According to the species and the multiplication process different systems were developped. Meristem proliferation (#Ananas#, #Musa#, #Saccharum#, #Xanthosoma# or microtuberisation (#Solanum#) was performed in large double vessels with an alternative transfer of the medium from one to the other. Rates of multiplication were far better that those obtained on semi-solid medium. For somatic embryogenesis more precise conditions are required and large volumes are not necessary. A specific apparatus was designed allowing for the expression, development and germination of embryos in the same vessel with very simple manipulations. Depending on the species (#Coffea#, #Hevea#, #Musa#) 1000 to 10000 embryos were obtained in a one liter vessel. The quality of the planlets allows a direct transfert to the nursery for growing and hardening.
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Subjects: | F30 - Génétique et amélioration des plantes, F02 - Multiplication végétative des plantes, F60 - Physiologie et biochimie végétale, Coffea, Musa, Hévéa, Embryogénèse somatique, technique de culture, culture in vitro, Immersion, http://aims.fao.org/aos/agrovoc/c_1720, http://aims.fao.org/aos/agrovoc/c_4993, http://aims.fao.org/aos/agrovoc/c_3588, http://aims.fao.org/aos/agrovoc/c_36911, http://aims.fao.org/aos/agrovoc/c_24396, http://aims.fao.org/aos/agrovoc/c_24135, http://aims.fao.org/aos/agrovoc/c_2316, |
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dig-cirad-fr-4011402024-01-28T01:12:13Z http://agritrop.cirad.fr/401140/ http://agritrop.cirad.fr/401140/ Temporary immersion for plant tissue culture. Teisson Claude, Alvard Daniel, Lartaud Marc, Etienne Hervé, Berthouly Marc, Escalona M., Lorenzo J.C.. 1998. In : Plant biotechnology and in vitro biology in the 21st century : abstracts = [Biotechnologie et biologie in vitro chez les plantes au 21ème siècle]. IAPTC. Jérusalem : IAPTC, 50. International Congress on Plant Tissue and Cell Culture. 9, Jérusalem, Israël, 14 Juin 1998/19 Juin 1998. Temporary immersion for plant tissue culture Teisson, Claude Alvard, Daniel Lartaud, Marc Etienne, Hervé Berthouly, Marc Escalona, M. Lorenzo, J.C. eng 1998 IAPTC Plant biotechnology and in vitro biology in the 21st century : abstracts = [Biotechnologie et biologie in vitro chez les plantes au 21ème siècle] F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 The use of temporary immersion in a liquid medium has been shown to improve plant micropropagation both for quality and economical aspects. The efficiency of the system originates in the improvement of nutrionnal and gazeous exchanges. Periods of immersion should be very brief and were easily controlled by a quick displacement of liquid through the application of an overpressure. According to the species and the multiplication process different systems were developped. Meristem proliferation (#Ananas#, #Musa#, #Saccharum#, #Xanthosoma# or microtuberisation (#Solanum#) was performed in large double vessels with an alternative transfer of the medium from one to the other. Rates of multiplication were far better that those obtained on semi-solid medium. For somatic embryogenesis more precise conditions are required and large volumes are not necessary. A specific apparatus was designed allowing for the expression, development and germination of embryos in the same vessel with very simple manipulations. Depending on the species (#Coffea#, #Hevea#, #Musa#) 1000 to 10000 embryos were obtained in a one liter vessel. The quality of the planlets allows a direct transfert to the nursery for growing and hardening. conference_item info:eu-repo/semantics/conferenceObject Conference info:eu-repo/semantics/closedAccess http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=98600 |
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F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 |
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F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 Teisson, Claude Alvard, Daniel Lartaud, Marc Etienne, Hervé Berthouly, Marc Escalona, M. Lorenzo, J.C. Temporary immersion for plant tissue culture |
description |
The use of temporary immersion in a liquid medium has been shown to improve plant micropropagation both for quality and economical aspects. The efficiency of the system originates in the improvement of nutrionnal and gazeous exchanges. Periods of immersion should be very brief and were easily controlled by a quick displacement of liquid through the application of an overpressure. According to the species and the multiplication process different systems were developped. Meristem proliferation (#Ananas#, #Musa#, #Saccharum#, #Xanthosoma# or microtuberisation (#Solanum#) was performed in large double vessels with an alternative transfer of the medium from one to the other. Rates of multiplication were far better that those obtained on semi-solid medium. For somatic embryogenesis more precise conditions are required and large volumes are not necessary. A specific apparatus was designed allowing for the expression, development and germination of embryos in the same vessel with very simple manipulations. Depending on the species (#Coffea#, #Hevea#, #Musa#) 1000 to 10000 embryos were obtained in a one liter vessel. The quality of the planlets allows a direct transfert to the nursery for growing and hardening. |
format |
conference_item |
topic_facet |
F30 - Génétique et amélioration des plantes F02 - Multiplication végétative des plantes F60 - Physiologie et biochimie végétale Coffea Musa Hévéa Embryogénèse somatique technique de culture culture in vitro Immersion http://aims.fao.org/aos/agrovoc/c_1720 http://aims.fao.org/aos/agrovoc/c_4993 http://aims.fao.org/aos/agrovoc/c_3588 http://aims.fao.org/aos/agrovoc/c_36911 http://aims.fao.org/aos/agrovoc/c_24396 http://aims.fao.org/aos/agrovoc/c_24135 http://aims.fao.org/aos/agrovoc/c_2316 |
author |
Teisson, Claude Alvard, Daniel Lartaud, Marc Etienne, Hervé Berthouly, Marc Escalona, M. Lorenzo, J.C. |
author_facet |
Teisson, Claude Alvard, Daniel Lartaud, Marc Etienne, Hervé Berthouly, Marc Escalona, M. Lorenzo, J.C. |
author_sort |
Teisson, Claude |
title |
Temporary immersion for plant tissue culture |
title_short |
Temporary immersion for plant tissue culture |
title_full |
Temporary immersion for plant tissue culture |
title_fullStr |
Temporary immersion for plant tissue culture |
title_full_unstemmed |
Temporary immersion for plant tissue culture |
title_sort |
temporary immersion for plant tissue culture |
publisher |
IAPTC |
url |
http://agritrop.cirad.fr/401140/ |
work_keys_str_mv |
AT teissonclaude temporaryimmersionforplanttissueculture AT alvarddaniel temporaryimmersionforplanttissueculture AT lartaudmarc temporaryimmersionforplanttissueculture AT etienneherve temporaryimmersionforplanttissueculture AT berthoulymarc temporaryimmersionforplanttissueculture AT escalonam temporaryimmersionforplanttissueculture AT lorenzojc temporaryimmersionforplanttissueculture |
_version_ |
1792492047277490176 |