Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree
The purpose of this study was to establish a fast system for producing transgenic actinorhizal root nodules of Casuarina glauca. Agrobacterium rhizogenes strain A4RS carrying the p35S-gusA-int gene construct was used to induce hairy roots on hypocotyls of 3-week-old C. glauca seedlings. Three weeks after wounding, the original root system was excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred to test tubes to be inoculated with Frankia. The actinorhizal nodules formed on transformed roots had the nitrogenase activity and morphology of untransformed nodules. B-GIucuronidase (GUS) activity was examined in transgenic roots and nodules by fluorometric and histochemical assays. The results indicate that transgenic nodules generated with this root transformation system could facilitate the molecular study of symbiotic nitrogen fixation in actinorhizal trees.
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Subjects: | P34 - Biologie du sol, gène, nodosité racinaire, http://aims.fao.org/aos/agrovoc/c_3214, http://aims.fao.org/aos/agrovoc/c_27601, |
Online Access: | http://agritrop.cirad.fr/390831/ http://agritrop.cirad.fr/390831/1/ID390831.pdf |
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dig-cirad-fr-3908312024-01-27T23:36:16Z http://agritrop.cirad.fr/390831/ http://agritrop.cirad.fr/390831/ Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree. Diouf Diégane, Gherbi H., Prin Yves, Franche Claudine, Duhoux Emile, Bogusz Didier. 1995. Molecular Plant-Microbe Interactions, 8 (4) : 532-537.https://doi.org/10.1094/MPMI-8-0532 <https://doi.org/10.1094/MPMI-8-0532> Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree Diouf, Diégane Gherbi, H. Prin, Yves Franche, Claudine Duhoux, Emile Bogusz, Didier eng 1995 Molecular Plant-Microbe Interactions P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 The purpose of this study was to establish a fast system for producing transgenic actinorhizal root nodules of Casuarina glauca. Agrobacterium rhizogenes strain A4RS carrying the p35S-gusA-int gene construct was used to induce hairy roots on hypocotyls of 3-week-old C. glauca seedlings. Three weeks after wounding, the original root system was excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred to test tubes to be inoculated with Frankia. The actinorhizal nodules formed on transformed roots had the nitrogenase activity and morphology of untransformed nodules. B-GIucuronidase (GUS) activity was examined in transgenic roots and nodules by fluorometric and histochemical assays. The results indicate that transgenic nodules generated with this root transformation system could facilitate the molecular study of symbiotic nitrogen fixation in actinorhizal trees. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/390831/1/ID390831.pdf text Cirad license info:eu-repo/semantics/restrictedAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1094/MPMI-8-0532 10.1094/MPMI-8-0532 http://catalogue-bibliotheques.cirad.fr/cgi-bin/koha/opac-detail.pl?biblionumber=90311 info:eu-repo/semantics/altIdentifier/doi/10.1094/MPMI-8-0532 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1094/MPMI-8-0532 |
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P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 |
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P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 Diouf, Diégane Gherbi, H. Prin, Yves Franche, Claudine Duhoux, Emile Bogusz, Didier Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
description |
The purpose of this study was to establish a fast system for producing transgenic actinorhizal root nodules of Casuarina glauca. Agrobacterium rhizogenes strain A4RS carrying the p35S-gusA-int gene construct was used to induce hairy roots on hypocotyls of 3-week-old C. glauca seedlings. Three weeks after wounding, the original root system was excised, and composite plants consisting of transgenic roots on untransformed shoots were transferred to test tubes to be inoculated with Frankia. The actinorhizal nodules formed on transformed roots had the nitrogenase activity and morphology of untransformed nodules. B-GIucuronidase (GUS) activity was examined in transgenic roots and nodules by fluorometric and histochemical assays. The results indicate that transgenic nodules generated with this root transformation system could facilitate the molecular study of symbiotic nitrogen fixation in actinorhizal trees. |
format |
article |
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P34 - Biologie du sol gène nodosité racinaire http://aims.fao.org/aos/agrovoc/c_3214 http://aims.fao.org/aos/agrovoc/c_27601 |
author |
Diouf, Diégane Gherbi, H. Prin, Yves Franche, Claudine Duhoux, Emile Bogusz, Didier |
author_facet |
Diouf, Diégane Gherbi, H. Prin, Yves Franche, Claudine Duhoux, Emile Bogusz, Didier |
author_sort |
Diouf, Diégane |
title |
Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
title_short |
Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
title_full |
Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
title_fullStr |
Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
title_full_unstemmed |
Hairy root nodulation of Casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
title_sort |
hairy root nodulation of casuarina glauca : a system for the study of symbiotic gene expression in an actinorhizal tree |
url |
http://agritrop.cirad.fr/390831/ http://agritrop.cirad.fr/390831/1/ID390831.pdf |
work_keys_str_mv |
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