Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites
Binding and competition among Cry1Aa, Cry1Ac, and Cry1Ba toxins were analyzed quantitatively #in vitro# by using 125 I-labeled activated toxins and brush border membrane vesicles isolated from #Chilo suppressalis# larval midguts. The three toxins bound specifically to the midgut brush border vesicles. Direct binding experiments showed that Cry1Aa and Cry1Ba recognized a single class of binding sites with different affinities, whereas Cry1Aa recognized two classes of binding sites, one with a high affinity and low concentration and the other with a lower affinity but higher concentration. Competition experiments showed that toxins Cry1Ac and Cry1Ba shared a binding site in the #C. suppressalis# midgut membranes and that this site was also the low-affinity binding site for Cry1Aa.
Main Authors: | , , , , |
---|---|
Format: | article biblioteca |
Language: | eng |
Subjects: | H10 - Ravageurs des plantes, Bacillus thuringiensis, Chilo suppressalis, toxine bactérienne, réaction biochimique, réaction de fixation du complément, http://aims.fao.org/aos/agrovoc/c_761, http://aims.fao.org/aos/agrovoc/c_27157, http://aims.fao.org/aos/agrovoc/c_9047, http://aims.fao.org/aos/agrovoc/c_27462, http://aims.fao.org/aos/agrovoc/c_1792, |
Online Access: | http://agritrop.cirad.fr/389872/ http://agritrop.cirad.fr/389872/1/389872.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cirad-fr-389872 |
---|---|
record_format |
koha |
spelling |
dig-cirad-fr-3898722024-01-27T23:06:58Z http://agritrop.cirad.fr/389872/ http://agritrop.cirad.fr/389872/ Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites. Fiuza Lidia Mariana, Nielsen Leroux Christina, Gozé Eric, Frutos Roger, Charles Jean François. 1996. Applied and Environmental Microbiology, 62 (5) : 1544-1549.https://doi.org/10.1128/AEM.62.5.1544-1549.1996 <https://doi.org/10.1128/AEM.62.5.1544-1549.1996> Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites Fiuza, Lidia Mariana Nielsen Leroux, Christina Gozé, Eric Frutos, Roger Charles, Jean François eng 1996 Applied and Environmental Microbiology H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 Binding and competition among Cry1Aa, Cry1Ac, and Cry1Ba toxins were analyzed quantitatively #in vitro# by using 125 I-labeled activated toxins and brush border membrane vesicles isolated from #Chilo suppressalis# larval midguts. The three toxins bound specifically to the midgut brush border vesicles. Direct binding experiments showed that Cry1Aa and Cry1Ba recognized a single class of binding sites with different affinities, whereas Cry1Aa recognized two classes of binding sites, one with a high affinity and low concentration and the other with a lower affinity but higher concentration. Competition experiments showed that toxins Cry1Ac and Cry1Ba shared a binding site in the #C. suppressalis# midgut membranes and that this site was also the low-affinity binding site for Cry1Aa. article info:eu-repo/semantics/article Journal Article info:eu-repo/semantics/publishedVersion http://agritrop.cirad.fr/389872/1/389872.pdf text Cirad license info:eu-repo/semantics/openAccess https://agritrop.cirad.fr/mention_legale.html https://doi.org/10.1128/AEM.62.5.1544-1549.1996 10.1128/AEM.62.5.1544-1549.1996 info:eu-repo/semantics/altIdentifier/doi/10.1128/AEM.62.5.1544-1549.1996 info:eu-repo/semantics/altIdentifier/purl/https://doi.org/10.1128/AEM.62.5.1544-1549.1996 |
institution |
CIRAD FR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cirad-fr |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CIRAD Francia |
language |
eng |
topic |
H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 |
spellingShingle |
H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 Fiuza, Lidia Mariana Nielsen Leroux, Christina Gozé, Eric Frutos, Roger Charles, Jean François Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
description |
Binding and competition among Cry1Aa, Cry1Ac, and Cry1Ba toxins were analyzed quantitatively #in vitro# by using 125 I-labeled activated toxins and brush border membrane vesicles isolated from #Chilo suppressalis# larval midguts. The three toxins bound specifically to the midgut brush border vesicles. Direct binding experiments showed that Cry1Aa and Cry1Ba recognized a single class of binding sites with different affinities, whereas Cry1Aa recognized two classes of binding sites, one with a high affinity and low concentration and the other with a lower affinity but higher concentration. Competition experiments showed that toxins Cry1Ac and Cry1Ba shared a binding site in the #C. suppressalis# midgut membranes and that this site was also the low-affinity binding site for Cry1Aa. |
format |
article |
topic_facet |
H10 - Ravageurs des plantes Bacillus thuringiensis Chilo suppressalis toxine bactérienne réaction biochimique réaction de fixation du complément http://aims.fao.org/aos/agrovoc/c_761 http://aims.fao.org/aos/agrovoc/c_27157 http://aims.fao.org/aos/agrovoc/c_9047 http://aims.fao.org/aos/agrovoc/c_27462 http://aims.fao.org/aos/agrovoc/c_1792 |
author |
Fiuza, Lidia Mariana Nielsen Leroux, Christina Gozé, Eric Frutos, Roger Charles, Jean François |
author_facet |
Fiuza, Lidia Mariana Nielsen Leroux, Christina Gozé, Eric Frutos, Roger Charles, Jean François |
author_sort |
Fiuza, Lidia Mariana |
title |
Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
title_short |
Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
title_full |
Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
title_fullStr |
Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
title_full_unstemmed |
Binding of Bacillus thuringiensis Cry1 toxins to the midgut brush border membrane vesicles of Chilo suppressalis (Lepidoptera : Pyralidae) : evidence of shared binding sites |
title_sort |
binding of bacillus thuringiensis cry1 toxins to the midgut brush border membrane vesicles of chilo suppressalis (lepidoptera : pyralidae) : evidence of shared binding sites |
url |
http://agritrop.cirad.fr/389872/ http://agritrop.cirad.fr/389872/1/389872.pdf |
work_keys_str_mv |
AT fiuzalidiamariana bindingofbacillusthuringiensiscry1toxinstothemidgutbrushbordermembranevesiclesofchilosuppressalislepidopterapyralidaeevidenceofsharedbindingsites AT nielsenlerouxchristina bindingofbacillusthuringiensiscry1toxinstothemidgutbrushbordermembranevesiclesofchilosuppressalislepidopterapyralidaeevidenceofsharedbindingsites AT gozeeric bindingofbacillusthuringiensiscry1toxinstothemidgutbrushbordermembranevesiclesofchilosuppressalislepidopterapyralidaeevidenceofsharedbindingsites AT frutosroger bindingofbacillusthuringiensiscry1toxinstothemidgutbrushbordermembranevesiclesofchilosuppressalislepidopterapyralidaeevidenceofsharedbindingsites AT charlesjeanfrancois bindingofbacillusthuringiensiscry1toxinstothemidgutbrushbordermembranevesiclesofchilosuppressalislepidopterapyralidaeevidenceofsharedbindingsites |
_version_ |
1792491250095489024 |