Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat
Terminal drought stress poses a big challenge to sustain wheat grain production in rain-fed environments. This study aimed to utilize the genetically diverse pre-breeding lines for identification of genomic regions associated with agro-physiological traits at terminal stage drought stress in wheat. A total of 339 pre-breeding lines panel derived from three-way crosses of 'exotics x elite x elite' lines were evaluated in field conditions at Obregon, Mexico for two years under well irrigated as well as drought stress environments. Drought stress was imposed at flowering by skipping the irrigations at pre and post anthesis stage. Results revealed that drought significantly reduced grain yield (Y), spike length (SL), number of grains spikes(-1) (NGS) and thousand kernel weight (TKW), while kernel abortion (KA) was increased. Population structure analysis in this panel uncovered three sub-populations. Genome wide linkage disequilibrium (LD) decay was observed at 2.5 centimorgan (cM). The haplotypes-based genome wide association study (GWAS) identified significant associations of Y, SL, and TKW on three chromosomes; 4A (HB10.7), 2D (HB6.10) and 3B (HB8.12), respectively. Likewise, associations on chromosomes 6B (HB17.1) and 3A (HB7.11) were found for NGS while on chromosome 3A (HB7.12) for KA. The genomic analysis information generated in the study can be efficiently utilized to improve Y and/or related parameters under terminal stage drought stress through marker-assisted breeding.
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dig-cimmyt-10883-208962021-02-24T15:51:44Z Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat Sajid Shokat Sehgal, D. Vikram, P. Fulai Liu Sukhwinder-Singh SOFT WHEAT GENETIC MARKERS GRAIN KERNELS Terminal drought stress poses a big challenge to sustain wheat grain production in rain-fed environments. This study aimed to utilize the genetically diverse pre-breeding lines for identification of genomic regions associated with agro-physiological traits at terminal stage drought stress in wheat. A total of 339 pre-breeding lines panel derived from three-way crosses of 'exotics x elite x elite' lines were evaluated in field conditions at Obregon, Mexico for two years under well irrigated as well as drought stress environments. Drought stress was imposed at flowering by skipping the irrigations at pre and post anthesis stage. Results revealed that drought significantly reduced grain yield (Y), spike length (SL), number of grains spikes(-1) (NGS) and thousand kernel weight (TKW), while kernel abortion (KA) was increased. Population structure analysis in this panel uncovered three sub-populations. Genome wide linkage disequilibrium (LD) decay was observed at 2.5 centimorgan (cM). The haplotypes-based genome wide association study (GWAS) identified significant associations of Y, SL, and TKW on three chromosomes; 4A (HB10.7), 2D (HB6.10) and 3B (HB8.12), respectively. Likewise, associations on chromosomes 6B (HB17.1) and 3A (HB7.11) were found for NGS while on chromosome 3A (HB7.12) for KA. The genomic analysis information generated in the study can be efficiently utilized to improve Y and/or related parameters under terminal stage drought stress through marker-assisted breeding. 2020-06-20T00:20:17Z 2020-06-20T00:20:17Z 2020 Article Published Version 1422-0067 (Print) https://hdl.handle.net/10883/20896 10.3390/ijms21093156 English https://www.mdpi.com/1422-0067/21/9/3156#supplementary CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Basel (Switzerland) MDPI 9 art. 3156 21 International Journal of Molecular Sciences |
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SOFT WHEAT GENETIC MARKERS GRAIN KERNELS SOFT WHEAT GENETIC MARKERS GRAIN KERNELS Sajid Shokat Sehgal, D. Vikram, P. Fulai Liu Sukhwinder-Singh Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
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Terminal drought stress poses a big challenge to sustain wheat grain production in rain-fed environments. This study aimed to utilize the genetically diverse pre-breeding lines for identification of genomic regions associated with agro-physiological traits at terminal stage drought stress in wheat. A total of 339 pre-breeding lines panel derived from three-way crosses of 'exotics x elite x elite' lines were evaluated in field conditions at Obregon, Mexico for two years under well irrigated as well as drought stress environments. Drought stress was imposed at flowering by skipping the irrigations at pre and post anthesis stage. Results revealed that drought significantly reduced grain yield (Y), spike length (SL), number of grains spikes(-1) (NGS) and thousand kernel weight (TKW), while kernel abortion (KA) was increased. Population structure analysis in this panel uncovered three sub-populations. Genome wide linkage disequilibrium (LD) decay was observed at 2.5 centimorgan (cM). The haplotypes-based genome wide association study (GWAS) identified significant associations of Y, SL, and TKW on three chromosomes; 4A (HB10.7), 2D (HB6.10) and 3B (HB8.12), respectively. Likewise, associations on chromosomes 6B (HB17.1) and 3A (HB7.11) were found for NGS while on chromosome 3A (HB7.12) for KA. The genomic analysis information generated in the study can be efficiently utilized to improve Y and/or related parameters under terminal stage drought stress through marker-assisted breeding. |
format |
Article |
topic_facet |
SOFT WHEAT GENETIC MARKERS GRAIN KERNELS |
author |
Sajid Shokat Sehgal, D. Vikram, P. Fulai Liu Sukhwinder-Singh |
author_facet |
Sajid Shokat Sehgal, D. Vikram, P. Fulai Liu Sukhwinder-Singh |
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Sajid Shokat |
title |
Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
title_short |
Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
title_full |
Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
title_fullStr |
Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
title_full_unstemmed |
Molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
title_sort |
molecular markers associated with agro-physiological traits under terminal drought conditions in bread wheat |
publisher |
MDPI |
publishDate |
2020 |
url |
https://hdl.handle.net/10883/20896 |
work_keys_str_mv |
AT sajidshokat molecularmarkersassociatedwithagrophysiologicaltraitsunderterminaldroughtconditionsinbreadwheat AT sehgald molecularmarkersassociatedwithagrophysiologicaltraitsunderterminaldroughtconditionsinbreadwheat AT vikramp molecularmarkersassociatedwithagrophysiologicaltraitsunderterminaldroughtconditionsinbreadwheat AT fulailiu molecularmarkersassociatedwithagrophysiologicaltraitsunderterminaldroughtconditionsinbreadwheat AT sukhwindersingh molecularmarkersassociatedwithagrophysiologicaltraitsunderterminaldroughtconditionsinbreadwheat |
_version_ |
1756086915474391040 |