GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm
This study was initiated to identify genomic regions conferring resistance to Karnal Bunt (KB) disease in wheat through a genome-wide association study (GWAS) on a set of 179 pre-breeding lines (PBLs). A GWAS of 6,382 high-quality DArTseq SNPs revealed 15 significant SNPs (P-value <10−3) on chromosomes 2D, 3B, 4D and 7B that were associated with KB resistance in individual years. In particular, two SNPs (chromosome 4D) had the maximum R2 values: SNP 1114200 | F | 0–63:T > C at 1.571 cM and R2 of 12.49% and SNP 1103052 | F | 0–61:C > A at 1.574 cM and R2 of 9.02%. These two SNPs displayed strong linkage disequilibrium (LD). An in silico analysis of SNPs on chromosome 4D identified two candidate gene hits, TraesCS4D02G352200 (TaNox8; an NADPH oxidase) and TraesCS4D02G350300 (a rhomboid-like protein belonging to family S54), with SNPs 1103052 | F | 0–61:C > A and 1101835 | F | 0–5:C > A, respectively, both of which function in biotic stress tolerance. The epistatic interaction analysis revealed significant interactions among 4D and 7B loci. A pedigree analysis of confirmed resistant PBLs revealed that Aegilops species is one of the parents and contributed the D genome in these resistant PBLs. These identified lines can be crossed with any elite cultivar across the globe to incorporate novel KB resistance identified on 4B.
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2020
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Subjects: | PLANT DISEASES, GENETIC RESISTANCE, SINGLE NUCLEOTIDE POLYMORPHISM, MARKER-ASSISTED SELECTION, WHEAT, |
Online Access: | https://hdl.handle.net/10883/20840 |
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dig-cimmyt-10883-208402023-12-05T14:20:47Z GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm Sukhwinder-Singh Sehgal, D. Satish Kumar Mian A.R. Arif Vikram, P. Sansaloni, C.P. Fuentes Dávila, G. Ortiz, C. PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT This study was initiated to identify genomic regions conferring resistance to Karnal Bunt (KB) disease in wheat through a genome-wide association study (GWAS) on a set of 179 pre-breeding lines (PBLs). A GWAS of 6,382 high-quality DArTseq SNPs revealed 15 significant SNPs (P-value <10−3) on chromosomes 2D, 3B, 4D and 7B that were associated with KB resistance in individual years. In particular, two SNPs (chromosome 4D) had the maximum R2 values: SNP 1114200 | F | 0–63:T > C at 1.571 cM and R2 of 12.49% and SNP 1103052 | F | 0–61:C > A at 1.574 cM and R2 of 9.02%. These two SNPs displayed strong linkage disequilibrium (LD). An in silico analysis of SNPs on chromosome 4D identified two candidate gene hits, TraesCS4D02G352200 (TaNox8; an NADPH oxidase) and TraesCS4D02G350300 (a rhomboid-like protein belonging to family S54), with SNPs 1103052 | F | 0–61:C > A and 1101835 | F | 0–5:C > A, respectively, both of which function in biotic stress tolerance. The epistatic interaction analysis revealed significant interactions among 4D and 7B loci. A pedigree analysis of confirmed resistant PBLs revealed that Aegilops species is one of the parents and contributed the D genome in these resistant PBLs. These identified lines can be crossed with any elite cultivar across the globe to incorporate novel KB resistance identified on 4B. 2020-04-25T00:10:16Z 2020-04-25T00:10:16Z 2020 Article Published Version 2045-2322 (Print) https://hdl.handle.net/10883/20840 10.1038/s41598-020-62711-7 English https://www.nature.com/articles/s41598-020-62711-7#Sec17 CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF London (United Kingdom) Nature Publishing Group 1 art. 5999 10 Nature Scientific Reports |
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PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT |
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PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT Sukhwinder-Singh Sehgal, D. Satish Kumar Mian A.R. Arif Vikram, P. Sansaloni, C.P. Fuentes Dávila, G. Ortiz, C. GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
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This study was initiated to identify genomic regions conferring resistance to Karnal Bunt (KB) disease in wheat through a genome-wide association study (GWAS) on a set of 179 pre-breeding lines (PBLs). A GWAS of 6,382 high-quality DArTseq SNPs revealed 15 significant SNPs (P-value <10−3) on chromosomes 2D, 3B, 4D and 7B that were associated with KB resistance in individual years. In particular, two SNPs (chromosome 4D) had the maximum R2 values: SNP 1114200 | F | 0–63:T > C at 1.571 cM and R2 of 12.49% and SNP 1103052 | F | 0–61:C > A at 1.574 cM and R2 of 9.02%. These two SNPs displayed strong linkage disequilibrium (LD). An in silico analysis of SNPs on chromosome 4D identified two candidate gene hits, TraesCS4D02G352200 (TaNox8; an NADPH oxidase) and TraesCS4D02G350300 (a rhomboid-like protein belonging to family S54), with SNPs 1103052 | F | 0–61:C > A and 1101835 | F | 0–5:C > A, respectively, both of which function in biotic stress tolerance. The epistatic interaction analysis revealed significant interactions among 4D and 7B loci. A pedigree analysis of confirmed resistant PBLs revealed that Aegilops species is one of the parents and contributed the D genome in these resistant PBLs. These identified lines can be crossed with any elite cultivar across the globe to incorporate novel KB resistance identified on 4B. |
format |
Article |
topic_facet |
PLANT DISEASES GENETIC RESISTANCE SINGLE NUCLEOTIDE POLYMORPHISM MARKER-ASSISTED SELECTION WHEAT |
author |
Sukhwinder-Singh Sehgal, D. Satish Kumar Mian A.R. Arif Vikram, P. Sansaloni, C.P. Fuentes Dávila, G. Ortiz, C. |
author_facet |
Sukhwinder-Singh Sehgal, D. Satish Kumar Mian A.R. Arif Vikram, P. Sansaloni, C.P. Fuentes Dávila, G. Ortiz, C. |
author_sort |
Sukhwinder-Singh |
title |
GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
title_short |
GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
title_full |
GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
title_fullStr |
GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
title_full_unstemmed |
GWAS revealed a novel resistance locus on chromosome 4D for the quarantine disease Karnal bunt in diverse wheat pre-breeding germplasm |
title_sort |
gwas revealed a novel resistance locus on chromosome 4d for the quarantine disease karnal bunt in diverse wheat pre-breeding germplasm |
publisher |
Nature Publishing Group |
publishDate |
2020 |
url |
https://hdl.handle.net/10883/20840 |
work_keys_str_mv |
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