Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources
Genetic resistance in the host plant is the most economical and environmentally friendly strategy for controlling wheat leaf rust, caused by Puccinia triticina Eriks. The durum wheat lines Gaza (Middle East), Arnacoris (France) and Saragolla (Italy) express high levels of resistance to the Mexican races of P. triticina. Three recombinant inbred line (RIL) populations, derived from crosses of each of these resistance sources to the susceptible line ATRED #2, were evaluated for leaf rust reactions at CIMMYT’s leaf rust nurseries in Mexico. Genetic analyses of host reactions suggested oligogenic control of resistance in all populations. The F8 RILs from each cross were genotyped using the Illumina iSelect 90K array, and high-density genetic maps were constructed for each population. Using composite interval mapping, a total of seven quantitative trait loci (QTL) that provide resistance to leaf rust were identified. Two QTL designated as QLr.usw-6BS and QLr.usw-6BL were identified on chromosome 6B in Gaza, which explained up to 78.5% and 21.3% of the observed leaf rust severity variance, respectively. A major QTL designated as QLr.usw-7BL was detected on the long arm of chromosome 7B in Arnacoris, which accounted for up to 65.9% of the disease severity variance. Arnacoris also carried a minor QTL on chromosome 1BL, designated as QLr.usw1BL.1 that explained up to 17.7% of the phenotypic variance. Three QTL conferred leaf rust resistance in Saragolla, namely QLr.usw-2BS, QLr.usw-3B, and QLr.usw-1BL.2, which accounted for up to 42.3, 9.4, and 7.1% of the phenotypic variance, respectively. Markers flanking each QTL were physically mapped against the durum wheat reference sequence and candidate genes involved in disease resistance were identified within the QTL intervals. The QTL identified in this study and their closely linked markers are useful resources for gene pyramiding and breeding for durable leaf rust resistance in durum wheat.
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2019
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, HARD WHEAT, RUSTS, PUCCINIA RECONDITA, DISEASE RESISTANCE, QUANTITATIVE TRAIT LOCI, SINGLE NUCLEOTIDE POLYMORPHISM, |
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dig-cimmyt-10883-205592021-03-31T14:21:13Z Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources Kthiri, D. Loladze, A. N’Diaye, A. Nilsen, K. Walkowiak, S. Dreisigacker, S. Ammar, K. Pozniak, C.J. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM Genetic resistance in the host plant is the most economical and environmentally friendly strategy for controlling wheat leaf rust, caused by Puccinia triticina Eriks. The durum wheat lines Gaza (Middle East), Arnacoris (France) and Saragolla (Italy) express high levels of resistance to the Mexican races of P. triticina. Three recombinant inbred line (RIL) populations, derived from crosses of each of these resistance sources to the susceptible line ATRED #2, were evaluated for leaf rust reactions at CIMMYT’s leaf rust nurseries in Mexico. Genetic analyses of host reactions suggested oligogenic control of resistance in all populations. The F8 RILs from each cross were genotyped using the Illumina iSelect 90K array, and high-density genetic maps were constructed for each population. Using composite interval mapping, a total of seven quantitative trait loci (QTL) that provide resistance to leaf rust were identified. Two QTL designated as QLr.usw-6BS and QLr.usw-6BL were identified on chromosome 6B in Gaza, which explained up to 78.5% and 21.3% of the observed leaf rust severity variance, respectively. A major QTL designated as QLr.usw-7BL was detected on the long arm of chromosome 7B in Arnacoris, which accounted for up to 65.9% of the disease severity variance. Arnacoris also carried a minor QTL on chromosome 1BL, designated as QLr.usw1BL.1 that explained up to 17.7% of the phenotypic variance. Three QTL conferred leaf rust resistance in Saragolla, namely QLr.usw-2BS, QLr.usw-3B, and QLr.usw-1BL.2, which accounted for up to 42.3, 9.4, and 7.1% of the phenotypic variance, respectively. Markers flanking each QTL were physically mapped against the durum wheat reference sequence and candidate genes involved in disease resistance were identified within the QTL intervals. The QTL identified in this study and their closely linked markers are useful resources for gene pyramiding and breeding for durable leaf rust resistance in durum wheat. art. 1247 2019-12-16T17:17:33Z 2019-12-16T17:17:33Z 2019 Article Published Version 1664-462X https://hdl.handle.net/10883/20559 10.3389/fpls.2019.01247 English https://figshare.com/collections/Mapping_of_Genetic_Loci_Conferring_Resistance_to_Leaf_Rust_From_Three_Globally_Resistant_Durum_Wheat_Sources/4690529 CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Switzerland Frontiers 10 Frontiers in Plant Science |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM Kthiri, D. Loladze, A. N’Diaye, A. Nilsen, K. Walkowiak, S. Dreisigacker, S. Ammar, K. Pozniak, C.J. Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
description |
Genetic resistance in the host plant is the most economical and environmentally friendly strategy for controlling wheat leaf rust, caused by Puccinia triticina Eriks. The durum wheat lines Gaza (Middle East), Arnacoris (France) and Saragolla (Italy) express high levels of resistance to the Mexican races of P. triticina. Three recombinant inbred line (RIL) populations, derived from crosses of each of these resistance sources to the susceptible line ATRED #2, were evaluated for leaf rust reactions at CIMMYT’s leaf rust nurseries in Mexico. Genetic analyses of host reactions suggested oligogenic control of resistance in all populations. The F8 RILs from each cross were genotyped using the Illumina iSelect 90K array, and high-density genetic maps were constructed for each population. Using composite interval mapping, a total of seven quantitative trait loci (QTL) that provide resistance to leaf rust were identified. Two QTL designated as QLr.usw-6BS and QLr.usw-6BL were identified on chromosome 6B in Gaza, which explained up to 78.5% and 21.3% of the observed leaf rust severity variance, respectively. A major QTL designated as QLr.usw-7BL was detected on the long arm of chromosome 7B in Arnacoris, which accounted for up to 65.9% of the disease severity variance. Arnacoris also carried a minor QTL on chromosome 1BL, designated as QLr.usw1BL.1 that explained up to 17.7% of the phenotypic variance. Three QTL conferred leaf rust resistance in Saragolla, namely QLr.usw-2BS, QLr.usw-3B, and QLr.usw-1BL.2, which accounted for up to 42.3, 9.4, and 7.1% of the phenotypic variance, respectively. Markers flanking each QTL were physically mapped against the durum wheat reference sequence and candidate genes involved in disease resistance were identified within the QTL intervals. The QTL identified in this study and their closely linked markers are useful resources for gene pyramiding and breeding for durable leaf rust resistance in durum wheat. |
format |
Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY HARD WHEAT RUSTS PUCCINIA RECONDITA DISEASE RESISTANCE QUANTITATIVE TRAIT LOCI SINGLE NUCLEOTIDE POLYMORPHISM |
author |
Kthiri, D. Loladze, A. N’Diaye, A. Nilsen, K. Walkowiak, S. Dreisigacker, S. Ammar, K. Pozniak, C.J. |
author_facet |
Kthiri, D. Loladze, A. N’Diaye, A. Nilsen, K. Walkowiak, S. Dreisigacker, S. Ammar, K. Pozniak, C.J. |
author_sort |
Kthiri, D. |
title |
Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
title_short |
Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
title_full |
Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
title_fullStr |
Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
title_full_unstemmed |
Mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
title_sort |
mapping of genetic loci conferring resistance to leaf rust from three globally resistant durum wheat sources |
publisher |
Frontiers |
publishDate |
2019 |
url |
https://hdl.handle.net/10883/20559 |
work_keys_str_mv |
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