Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.)
Plant cell wall formation is a complex, coordinated and developmentally regulated process. Cellulose is the most dominant constituent of plant cell walls. Because of its paracrystalline structure, cellulose is the main determinant of mechanical strength of plant tissues. As the most abundant polysaccharide on earth, it is also the focus of cellulosic biofuel industry. To reduce culm lodging in wheat and for improved ethanol production, delineation of the variation for stem cellulose content could prove useful. We present results on the analysis of the stem cellulose content of 288 diverse wheat accessions and its genome-wide association study (GWAS). Cellulose concentration ranged from 35 to 52% (w/w). Cellulose content was normally distributed in the accessions around a mean and median of 45% (w/w). Genome-wide marker-trait association study using 21,073 SNPs helped identify nine SNPs that were associated (p < 1E-05) with cellulose content. Four strongly associated (p < 8.17E-05) SNP markers were linked to wheat unigenes, which included β-tubulin, Auxin-induced protein 5NG4, and a putative transmembrane protein of unknown function. These genes may be directly or indirectly involved in the formation of cellulose in wheat culms. GWAS results from this study have the potential for genetic manipulation of cellulose content in bread wheat and other small grain cereals to enhance culm strength and improve biofuel production.
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Frontiers
2017
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, GWAS, SNPs, Cell Wall, GENOMES, CELLULOSE, WHEAT, CEREALS, GRASSES, SINGLE NUCLEOTIDE POLYMORPHISM, BIOENERGY, CELL WALLS, |
Online Access: | https://hdl.handle.net/10883/19433 |
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dig-cimmyt-10883-194332021-02-24T16:45:26Z Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) Kaur, S. Xu Zhang Mohan, A. Dong, H. Vikram, P. Sukhwinder-Singh Zhiwu Zhang Gill, K.S. Dhugga, K. Singh, J. AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS Plant cell wall formation is a complex, coordinated and developmentally regulated process. Cellulose is the most dominant constituent of plant cell walls. Because of its paracrystalline structure, cellulose is the main determinant of mechanical strength of plant tissues. As the most abundant polysaccharide on earth, it is also the focus of cellulosic biofuel industry. To reduce culm lodging in wheat and for improved ethanol production, delineation of the variation for stem cellulose content could prove useful. We present results on the analysis of the stem cellulose content of 288 diverse wheat accessions and its genome-wide association study (GWAS). Cellulose concentration ranged from 35 to 52% (w/w). Cellulose content was normally distributed in the accessions around a mean and median of 45% (w/w). Genome-wide marker-trait association study using 21,073 SNPs helped identify nine SNPs that were associated (p < 1E-05) with cellulose content. Four strongly associated (p < 8.17E-05) SNP markers were linked to wheat unigenes, which included β-tubulin, Auxin-induced protein 5NG4, and a putative transmembrane protein of unknown function. These genes may be directly or indirectly involved in the formation of cellulose in wheat culms. GWAS results from this study have the potential for genetic manipulation of cellulose content in bread wheat and other small grain cereals to enhance culm strength and improve biofuel production. 2018-05-04T17:06:49Z 2018-05-04T17:06:49Z 2017 Article https://hdl.handle.net/10883/19433 10.3389/fpls.2017.01913 English https://www.frontiersin.org/articles/file/downloadfile/275877_supplementary-materials_tables_2_docx/octet-stream/Supplementary%20Tables.docx/1/275877 CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Switzerland Frontiers 8 Frontiers in Plant Science 1913 |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS Kaur, S. Xu Zhang Mohan, A. Dong, H. Vikram, P. Sukhwinder-Singh Zhiwu Zhang Gill, K.S. Dhugga, K. Singh, J. Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
description |
Plant cell wall formation is a complex, coordinated and developmentally regulated process. Cellulose is the most dominant constituent of plant cell walls. Because of its paracrystalline structure, cellulose is the main determinant of mechanical strength of plant tissues. As the most abundant polysaccharide on earth, it is also the focus of cellulosic biofuel industry. To reduce culm lodging in wheat and for improved ethanol production, delineation of the variation for stem cellulose content could prove useful. We present results on the analysis of the stem cellulose content of 288 diverse wheat accessions and its genome-wide association study (GWAS). Cellulose concentration ranged from 35 to 52% (w/w). Cellulose content was normally distributed in the accessions around a mean and median of 45% (w/w). Genome-wide marker-trait association study using 21,073 SNPs helped identify nine SNPs that were associated (p < 1E-05) with cellulose content. Four strongly associated (p < 8.17E-05) SNP markers were linked to wheat unigenes, which included β-tubulin, Auxin-induced protein 5NG4, and a putative transmembrane protein of unknown function. These genes may be directly or indirectly involved in the formation of cellulose in wheat culms. GWAS results from this study have the potential for genetic manipulation of cellulose content in bread wheat and other small grain cereals to enhance culm strength and improve biofuel production. |
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Article |
topic_facet |
AGRICULTURAL SCIENCES AND BIOTECHNOLOGY GWAS SNPs Cell Wall GENOMES CELLULOSE WHEAT CEREALS GRASSES SINGLE NUCLEOTIDE POLYMORPHISM BIOENERGY CELL WALLS |
author |
Kaur, S. Xu Zhang Mohan, A. Dong, H. Vikram, P. Sukhwinder-Singh Zhiwu Zhang Gill, K.S. Dhugga, K. Singh, J. |
author_facet |
Kaur, S. Xu Zhang Mohan, A. Dong, H. Vikram, P. Sukhwinder-Singh Zhiwu Zhang Gill, K.S. Dhugga, K. Singh, J. |
author_sort |
Kaur, S. |
title |
Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
title_short |
Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
title_full |
Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
title_fullStr |
Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
title_full_unstemmed |
Genome-Wide Association study reveals novel genes associated with culm cellulose content in bread wheat (Triticum aestivum, L.) |
title_sort |
genome-wide association study reveals novel genes associated with culm cellulose content in bread wheat (triticum aestivum, l.) |
publisher |
Frontiers |
publishDate |
2017 |
url |
https://hdl.handle.net/10883/19433 |
work_keys_str_mv |
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