Bulked sample analysis in genetics, genomics and crop improvement
Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives.
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Format: | Article biblioteca |
Language: | English |
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Wiley Open Access
2016
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Subjects: | AGRICULTURAL SCIENCES AND BIOTECHNOLOGY, Bulked Sample Analysis, Bulked Segregant Analysis, Microarrays, Chips, DNA-seq, RNA-seq, Protein-seq, GENETICS, SAMPLING, DNA SEQUENCE, RNA SEQUENCE, BREEDING, |
Online Access: | http://hdl.handle.net/10883/18829 |
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dig-cimmyt-10883-188292023-11-17T20:29:41Z Bulked sample analysis in genetics, genomics and crop improvement Cheng Zou Pingxi Wang Yunbi Xu AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives. 1941-1955 2017-08-16T16:17:36Z 2017-08-16T16:17:36Z 2016 Article http://hdl.handle.net/10883/18829 10.1111/pbi.12559 English https://onlinelibrary.wiley.com/doi/full/10.1111/pbi.12559#support-information-section CIMMYT manages Intellectual Assets as International Public Goods. The user is free to download, print, store and share this work. In case you want to translate or create any other derivative work and share or distribute such translation/derivative work, please contact CIMMYT-Knowledge-Center@cgiar.org indicating the work you want to use and the kind of use you intend; CIMMYT will contact you with the suitable license for that purpose. Open Access PDF Oxford, United Kingdom Wiley Open Access Association of Applied Biologists Society for Experimental Biology Blackwell Publishing 10 14 Plant Biotechnology Journal |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING Cheng Zou Pingxi Wang Yunbi Xu Bulked sample analysis in genetics, genomics and crop improvement |
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Biological assay has been based on analysis of all individuals collected from sample populations. Bulked sample analysis (BSA), which works with selected and pooled individuals, has been extensively used in gene mapping through bulked segregant analysis with biparental populations, mapping by sequencing with major gene mutants and pooled genomewide association study using extreme variants. Compared to conventional entire population analysis, BSA significantly reduces the scale and cost by simplifying the procedure. The bulks can be built by selection of extremes or representative samples from any populations and all types of segregants and variants that represent wide ranges of phenotypic variation for the target trait. Methods and procedures for sampling, bulking and multiplexing are described. The samples can be analysed using individual markers, microarrays and high-throughput sequencing at all levels of DNA, RNA and protein. The power of BSA is affected by population size, selection of extreme individuals, sequencing strategies, genetic architecture of the trait and marker density. BSA will facilitate plant breeding through development of diagnostic and constitutive markers, agronomic genomics, marker-assisted selection and selective phenotyping. Applications of BSA in genetics, genomics and crop improvement are discussed with their future perspectives. |
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Article |
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AGRICULTURAL SCIENCES AND BIOTECHNOLOGY Bulked Sample Analysis Bulked Segregant Analysis Microarrays Chips DNA-seq RNA-seq Protein-seq GENETICS SAMPLING DNA SEQUENCE RNA SEQUENCE BREEDING |
author |
Cheng Zou Pingxi Wang Yunbi Xu |
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Cheng Zou Pingxi Wang Yunbi Xu |
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Cheng Zou |
title |
Bulked sample analysis in genetics, genomics and crop improvement |
title_short |
Bulked sample analysis in genetics, genomics and crop improvement |
title_full |
Bulked sample analysis in genetics, genomics and crop improvement |
title_fullStr |
Bulked sample analysis in genetics, genomics and crop improvement |
title_full_unstemmed |
Bulked sample analysis in genetics, genomics and crop improvement |
title_sort |
bulked sample analysis in genetics, genomics and crop improvement |
publisher |
Wiley Open Access |
publishDate |
2016 |
url |
http://hdl.handle.net/10883/18829 |
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AT chengzou bulkedsampleanalysisingeneticsgenomicsandcropimprovement AT pingxiwang bulkedsampleanalysisingeneticsgenomicsandcropimprovement AT yunbixu bulkedsampleanalysisingeneticsgenomicsandcropimprovement |
_version_ |
1787232929989001216 |