Sea turtle hatchling carapace as a source of high quantity and quality DNA
Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research.
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| Format: | info:eu-repo/semantics/article biblioteca |
| Language: | eng |
| Subjects: | info:eu-repo/classification/Autores/CARAPACE, info:eu-repo/classification/Autores/DNA EXTRACTION, info:eu-repo/classification/Autores/SEA TURTLE, info:eu-repo/classification/cti/2, |
| Online Access: | http://cicy.repositorioinstitucional.mx/jspui/handle/1003/495 |
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dig-cicy-1003-4952017-08-07T13:54:08Z Sea turtle hatchling carapace as a source of high quantity and quality DNA Uso del caparazón de crías de tortugas marinas como fuente de alta cantidad y calidad de ADN BLANCA IDALIA GONZALEZ GARZA LORENZO FELIPE SÁNCHEZ TEYER JOSE OMAR ZAPATA PEREZ 2016-04 info:eu-repo/semantics/article Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research. info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 Revista de Biologia Marina y Oceanografia. 51(1), 217-222, 2016 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/495 info:eu-repo/semantics/datasetDOI/http://dx.doi.org/10.4067/S0718-19572016000100024 info:eu-repo/semantics/openAccess eng citation:González-Garza, B. I., Sánchez-Teyer, L. F., & Zapata-Pérez, O. (2016). Sea turtle hatchling carapace as a source of high quantity and quality DNA. Revista de Biología Marina y Oceanografía, 51(1). http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
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info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 BLANCA IDALIA GONZALEZ GARZA Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| description |
Hatchling DNA provides valuable information on sea turtles. Samples can be obtained from dead hatchlings or embryo, or, when live animal samples are needed, from blood, flipper or carapace. We compared 120 DNA extractions from flipper and carapace tissue of dead and live hatchlings. There were significant differences in DNA yields from the different tissues, but no significant differences in DNA purity. Some flipper samples yielded low amounts of DNA, while the carapace tissue consistently produced high yields of good quality DNA. This suggests that carapace represents the best option for tissue sampling of hatchling sea turtles in genetic research. |
| author2 |
LORENZO FELIPE SÁNCHEZ TEYER |
| author_facet |
LORENZO FELIPE SÁNCHEZ TEYER BLANCA IDALIA GONZALEZ GARZA |
| format |
info:eu-repo/semantics/article |
| topic_facet |
info:eu-repo/classification/Autores/CARAPACE info:eu-repo/classification/Autores/DNA EXTRACTION info:eu-repo/classification/Autores/SEA TURTLE info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/2 |
| author |
BLANCA IDALIA GONZALEZ GARZA |
| author_sort |
BLANCA IDALIA GONZALEZ GARZA |
| title |
Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| title_short |
Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| title_full |
Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| title_fullStr |
Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| title_full_unstemmed |
Sea turtle hatchling carapace as a source of high quantity and quality DNA |
| title_sort |
sea turtle hatchling carapace as a source of high quantity and quality dna |
| url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/495 |
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