Musa genetic diversity revealed by SRAP and AFLP

The sequence-related amplified polymorphism (SRAP) technique, aimed for the amplification of open reading frames (ORFs), vis-â-vis that of the amplified fragment length polymorphisms (AFLP) were used to analyze the genetic variation and relationships among forty Musa accessions; which include commercial cultivars and wild species of interest for the genetic enhancement of Musa. A total of 403 SRAP and 837 AFLP amplicons were generated by 10 SRAP and 15 AFLP primer combinations, of which 353 and 787 bands were polymorphic, respectively. Both cluster analysis of unweighted pair-grouping method with arithmetic averages (UPGMA) and principal coordinate (PCO) analysis separated the forty accessions into their recognized sections (Eumusa, Australimusa, Callimusa and Rhodochlamys) and species. The percentage of polymorphism amongst sections and species and the relationships within Eumusa species and subspecies varied between the two marker systems. In addition to its practical simplicity, SRAP exhibited approximately threefold more specific and unique bands than AFLP, 37 and 13%, respectively. SRAP markers are demonstrated here to be proficient tools for discriminating amongst M. acuminata, M. balbisiana and M. schizocarpa in the Eumusa section, as well as between plantains and cooking bananas within triploid cultivars.

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Main Author: ROSA MARIA ESCOBEDO GRACIA MEDRANO
Format: info:eu-repo/semantics/article biblioteca
Language:eng
Subjects:info:eu-repo/classification/cti/6, info:eu-repo/classification/cti/31, info:eu-repo/classification/cti/3199, info:eu-repo/classification/cti/319999,
Online Access:http://cicy.repositorioinstitucional.mx/jspui/handle/1003/213
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spelling dig-cicy-1003-2132016-10-26T18:37:40Z Musa genetic diversity revealed by SRAP and AFLP ROSA MARIA ESCOBEDO GRACIA MEDRANO 2011-03-01 info:eu-repo/semantics/article The sequence-related amplified polymorphism (SRAP) technique, aimed for the amplification of open reading frames (ORFs), vis-â-vis that of the amplified fragment length polymorphisms (AFLP) were used to analyze the genetic variation and relationships among forty Musa accessions; which include commercial cultivars and wild species of interest for the genetic enhancement of Musa. A total of 403 SRAP and 837 AFLP amplicons were generated by 10 SRAP and 15 AFLP primer combinations, of which 353 and 787 bands were polymorphic, respectively. Both cluster analysis of unweighted pair-grouping method with arithmetic averages (UPGMA) and principal coordinate (PCO) analysis separated the forty accessions into their recognized sections (Eumusa, Australimusa, Callimusa and Rhodochlamys) and species. The percentage of polymorphism amongst sections and species and the relationships within Eumusa species and subspecies varied between the two marker systems. In addition to its practical simplicity, SRAP exhibited approximately threefold more specific and unique bands than AFLP, 37 and 13%, respectively. SRAP markers are demonstrated here to be proficient tools for discriminating amongst M. acuminata, M. balbisiana and M. schizocarpa in the Eumusa section, as well as between plantains and cooking bananas within triploid cultivars. librarians students researchers teachers info:eu-repo/classification/cti/6 info:eu-repo/classification/cti/31 info:eu-repo/classification/cti/3199 info:eu-repo/classification/cti/319999 info:eu-repo/classification/cti/319999 http://cicy.repositorioinstitucional.mx/jspui/handle/1003/213 info:eu-repo/semantics/datasetDOI/10.1007/s12033-010-9328-8 info:eu-repo/semantics/altIdentifier/DOI/10.1007/s12033-010-9328-8 info:eu-repo/semantics/openAccess eng info:eu-repo/semantics/reference/DOI/10.1007/s12033-010-9328-8 http://creativecommons.org/licenses/by-nc-nd/4.0 application/pdf
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country México
countrycode MX
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region America del Norte
libraryname Biblioteca del CICY
language eng
topic info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/31
info:eu-repo/classification/cti/3199
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/31
info:eu-repo/classification/cti/3199
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/319999
spellingShingle info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/31
info:eu-repo/classification/cti/3199
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/31
info:eu-repo/classification/cti/3199
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/319999
ROSA MARIA ESCOBEDO GRACIA MEDRANO
Musa genetic diversity revealed by SRAP and AFLP
description The sequence-related amplified polymorphism (SRAP) technique, aimed for the amplification of open reading frames (ORFs), vis-â-vis that of the amplified fragment length polymorphisms (AFLP) were used to analyze the genetic variation and relationships among forty Musa accessions; which include commercial cultivars and wild species of interest for the genetic enhancement of Musa. A total of 403 SRAP and 837 AFLP amplicons were generated by 10 SRAP and 15 AFLP primer combinations, of which 353 and 787 bands were polymorphic, respectively. Both cluster analysis of unweighted pair-grouping method with arithmetic averages (UPGMA) and principal coordinate (PCO) analysis separated the forty accessions into their recognized sections (Eumusa, Australimusa, Callimusa and Rhodochlamys) and species. The percentage of polymorphism amongst sections and species and the relationships within Eumusa species and subspecies varied between the two marker systems. In addition to its practical simplicity, SRAP exhibited approximately threefold more specific and unique bands than AFLP, 37 and 13%, respectively. SRAP markers are demonstrated here to be proficient tools for discriminating amongst M. acuminata, M. balbisiana and M. schizocarpa in the Eumusa section, as well as between plantains and cooking bananas within triploid cultivars.
format info:eu-repo/semantics/article
topic_facet info:eu-repo/classification/cti/6
info:eu-repo/classification/cti/31
info:eu-repo/classification/cti/3199
info:eu-repo/classification/cti/319999
info:eu-repo/classification/cti/319999
author ROSA MARIA ESCOBEDO GRACIA MEDRANO
author_facet ROSA MARIA ESCOBEDO GRACIA MEDRANO
author_sort ROSA MARIA ESCOBEDO GRACIA MEDRANO
title Musa genetic diversity revealed by SRAP and AFLP
title_short Musa genetic diversity revealed by SRAP and AFLP
title_full Musa genetic diversity revealed by SRAP and AFLP
title_fullStr Musa genetic diversity revealed by SRAP and AFLP
title_full_unstemmed Musa genetic diversity revealed by SRAP and AFLP
title_sort musa genetic diversity revealed by srap and aflp
url http://cicy.repositorioinstitucional.mx/jspui/handle/1003/213
work_keys_str_mv AT rosamariaescobedograciamedrano musageneticdiversityrevealedbysrapandaflp
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