Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense
Fusarium equiseti strain FCHE and Fusarium oxysporum strain FCHJ were isolated from the roots of wilting habanero pepper (Capsicum chinense Jacq.) seedlings with root rot. Toward developing a biorational control of these serious phytopathogenic strains, ethanolic (EE) and aqueous (AE) extracts of different vegetative parts of 40 tropical native plants of the Yucatán Peninsula were screened for antifungal activity. Extracts of six out of 40 assayed plants were effective, and the most inhibitory extracts were studied further. EEs from Mosannona depressa (bark from stems and roots), Parathesis cubana (roots), and Piper neesianum (leaves) inhibited mycelial growth of both strains. Each active EE was then partitioned between hexane and acetonitrile. The acetonitrile fraction from M. depressa stem bark (MDT-b) had the lowest minimum inhibitory concentration of 1000 µg/mL against both pathogens and moderate inhibitory concentration (IC50) of 462 against F. equiseti and 472 µg/mL against F. oxysporum. After 96 h treatment with EE from M. depressa stem bark, both strains had distorted hyphae and conidia and collapsed conidia in scanning electron micrographs. Liquid chromatography–ultraviolet–high resolution mass spectrometry analysis revealed that the major component of the fraction was α-asarone. Its antifungal effect was verified using a commercial standard, which had an IC50 of 236 µg/mL against F. equiseti and >500 µg/mL against F. oxysporum. Furthermore, the P. cubana hexane fraction and P. neesianum acetonitrile fraction had antifungal activity against both Fusarium pathogens. These compounds provide new options for biorational products to control phytopathogenic fungi.
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Subjects: | info:eu-repo/classification/Autores/ANTIFUNGAL, info:eu-repo/classification/Autores/Α-ASARONE, info:eu-repo/classification/Autores/HABANERO PEPPER, info:eu-repo/classification/Autores/PHYTOPATHOGENS, info:eu-repo/classification/Autores/MOSANNONA DEPRESSA, info:eu-repo/classification/Autores/PLANT EXTRACTS, info:eu-repo/classification/cti/2, info:eu-repo/classification/cti/24, info:eu-repo/classification/cti/2415, info:eu-repo/classification/cti/241502, |
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dig-cicy-1003-18732021-09-28T16:23:33Z Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense PATRICIA CRUZ CERINO Jairo Cristóbal Alejo VIOLETA RUIZ CARRERA GERMAN CARNEVALI FERNANDEZ CONCHA BLANCA MARINA VERA KU Jesús Martin Fernando Reyes MARIA MARCELA GAMBOA ANGULO 2020 info:eu-repo/semantics/article Fusarium equiseti strain FCHE and Fusarium oxysporum strain FCHJ were isolated from the roots of wilting habanero pepper (Capsicum chinense Jacq.) seedlings with root rot. Toward developing a biorational control of these serious phytopathogenic strains, ethanolic (EE) and aqueous (AE) extracts of different vegetative parts of 40 tropical native plants of the Yucatán Peninsula were screened for antifungal activity. Extracts of six out of 40 assayed plants were effective, and the most inhibitory extracts were studied further. EEs from Mosannona depressa (bark from stems and roots), Parathesis cubana (roots), and Piper neesianum (leaves) inhibited mycelial growth of both strains. Each active EE was then partitioned between hexane and acetonitrile. The acetonitrile fraction from M. depressa stem bark (MDT-b) had the lowest minimum inhibitory concentration of 1000 µg/mL against both pathogens and moderate inhibitory concentration (IC50) of 462 against F. equiseti and 472 µg/mL against F. oxysporum. After 96 h treatment with EE from M. depressa stem bark, both strains had distorted hyphae and conidia and collapsed conidia in scanning electron micrographs. Liquid chromatography–ultraviolet–high resolution mass spectrometry analysis revealed that the major component of the fraction was α-asarone. Its antifungal effect was verified using a commercial standard, which had an IC50 of 236 µg/mL against F. equiseti and >500 µg/mL against F. oxysporum. Furthermore, the P. cubana hexane fraction and P. neesianum acetonitrile fraction had antifungal activity against both Fusarium pathogens. These compounds provide new options for biorational products to control phytopathogenic fungi. info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 Pathogens, 9(10), 827, 2020. http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1873 info:eu-repo/semantics/datasetDOI/10.3390/pathogens9100827 info:eu-repo/semantics/openAccess eng citation:Cruz-Cerino, P., Cristóbal-Alejo, J., Ruiz-Carrera, V., Carnevali, G., Vera-Ku, M., Martín, J., ... & Gamboa-Angulo, M. (2020). Extracts from Six Native Plants of the Yucatán Peninsula Hinder Mycelial Growth of Fusarium equiseti and F. oxysporum, Pathogens of Capsicum chinense. Pathogens, 9(10), 827. http://creativecommons.org/licenses/by-nc-nd/4.0 info:eu-repo/semantics/publishedVersion application/pdf |
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info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 |
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info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 PATRICIA CRUZ CERINO Jairo Cristóbal Alejo VIOLETA RUIZ CARRERA GERMAN CARNEVALI FERNANDEZ CONCHA BLANCA MARINA VERA KU Jesús Martin Fernando Reyes MARIA MARCELA GAMBOA ANGULO Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
description |
Fusarium equiseti strain FCHE and Fusarium oxysporum strain FCHJ were isolated from the roots of wilting habanero pepper (Capsicum chinense Jacq.) seedlings with root rot. Toward developing a biorational control of these serious phytopathogenic strains, ethanolic (EE) and aqueous (AE) extracts of different vegetative parts of 40 tropical native plants of the Yucatán Peninsula were screened for antifungal activity. Extracts of six out of 40 assayed plants were effective, and the most inhibitory extracts were studied further. EEs from Mosannona depressa (bark from stems and roots), Parathesis cubana (roots), and Piper neesianum (leaves) inhibited mycelial growth of both strains. Each active EE was then partitioned between hexane and acetonitrile. The acetonitrile fraction from M. depressa stem bark (MDT-b) had the lowest minimum inhibitory concentration of 1000 µg/mL against both pathogens and moderate inhibitory concentration (IC50) of 462 against F. equiseti and 472 µg/mL against F. oxysporum. After 96 h treatment with EE from M. depressa stem bark, both strains had distorted hyphae and conidia and collapsed conidia in scanning electron micrographs. Liquid chromatography–ultraviolet–high resolution mass spectrometry analysis revealed that the major component of the fraction was α-asarone. Its antifungal effect was verified using a commercial standard, which had an IC50 of 236 µg/mL against F. equiseti and >500 µg/mL against F. oxysporum. Furthermore, the P. cubana hexane fraction and P. neesianum acetonitrile fraction had antifungal activity against both Fusarium pathogens. These compounds provide new options for biorational products to control phytopathogenic fungi. |
format |
info:eu-repo/semantics/article |
topic_facet |
info:eu-repo/classification/Autores/ANTIFUNGAL info:eu-repo/classification/Autores/Α-ASARONE info:eu-repo/classification/Autores/HABANERO PEPPER info:eu-repo/classification/Autores/PHYTOPATHOGENS info:eu-repo/classification/Autores/MOSANNONA DEPRESSA info:eu-repo/classification/Autores/PLANT EXTRACTS info:eu-repo/classification/cti/2 info:eu-repo/classification/cti/24 info:eu-repo/classification/cti/2415 info:eu-repo/classification/cti/241502 info:eu-repo/classification/cti/241502 |
author |
PATRICIA CRUZ CERINO Jairo Cristóbal Alejo VIOLETA RUIZ CARRERA GERMAN CARNEVALI FERNANDEZ CONCHA BLANCA MARINA VERA KU Jesús Martin Fernando Reyes MARIA MARCELA GAMBOA ANGULO |
author_facet |
PATRICIA CRUZ CERINO Jairo Cristóbal Alejo VIOLETA RUIZ CARRERA GERMAN CARNEVALI FERNANDEZ CONCHA BLANCA MARINA VERA KU Jesús Martin Fernando Reyes MARIA MARCELA GAMBOA ANGULO |
author_sort |
PATRICIA CRUZ CERINO |
title |
Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
title_short |
Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
title_full |
Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
title_fullStr |
Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
title_full_unstemmed |
Extracts from six native plants of the Yucatan Peninsula hinder mycelial growth of Fusarium equiseti and F. oxysporum, pathogens of Capsicum chinense |
title_sort |
extracts from six native plants of the yucatan peninsula hinder mycelial growth of fusarium equiseti and f. oxysporum, pathogens of capsicum chinense |
url |
http://cicy.repositorioinstitucional.mx/jspui/handle/1003/1873 |
work_keys_str_mv |
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