Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance
Resumen del póster presentado al 28th International Symposium on Chromatography celebrado en Valencia (España) del 12 al 16 de septiembre de 2010.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | póster de congreso biblioteca |
| Published: |
2010
|
| Online Access: | http://hdl.handle.net/10261/113929 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-cial-es-10261-113929 |
|---|---|
| record_format |
koha |
| spelling |
dig-cial-es-10261-1139292018-08-31T09:46:14Z Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance Bernal, José Herrero, Miguel Velasco, Diego Elvira, Carlos Cifuentes, Alejandro Resumen del póster presentado al 28th International Symposium on Chromatography celebrado en Valencia (España) del 12 al 16 de septiembre de 2010. In capillary electrophoresis (CE), fused-silica capillary surface has long been known to cause adsorption problems during the analysis of high positively charged compounds (e.g., basic proteins) due to electrostatic interactions between the solute and the capillary wall. Moreover, the fused silica wall is also responsible of the irreproducibility frequently observed between injections and capillaries. The use of capillary coatings is a good strategy to avoid these harmful effects. In this work, the properties of four copolymers synthesized in our laboratory are studied as physically adsorbed coatings for capillary electrophoresis (CE). Namely, the four copolymers investigated were poly(N ethyl morpholine methacrylamide-co-N,N-dimethyl-acrylamide), poly(N ethyl pyrrolidine methacrylate-co-N,N-dimethylacrylamide), poly(N ethyl morpholine methacrylate-co-N,N-dimethylacrylamide) and poly(N ethyl pyrrolidine methacrylamide-co-N,N-dimethylacrylamide). The capillary coating is easily obtained by simply flushing into the tubing the dissolution containing the copolymer. The stability and reproducibility of each coating were tested for the same day, different days and different capillaries. It is demonstrated that the use of these coatings in CE can drastically reduce the analysis time and/or to improve the reproducibility of the separations. It is also shown that all the coated capillaries allow the separation of basic proteins by reducing their adsorption onto the capillary wall. Links between their molecular structure, physico-chemical properties and their performance as coatings in CE are discussed. Peer Reviewed 2015-04-21T11:04:55Z 2015-04-21T11:04:55Z 2010 2015-04-21T11:04:55Z póster de congreso http://purl.org/coar/resource_type/c_6670 ISC 2010 http://hdl.handle.net/10261/113929 Sí none |
| institution |
CIAL ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-cial-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del CIAL España |
| description |
Resumen del póster presentado al 28th International Symposium on Chromatography celebrado en Valencia (España) del 12 al 16 de septiembre de 2010. |
| format |
póster de congreso |
| author |
Bernal, José Herrero, Miguel Velasco, Diego Elvira, Carlos Cifuentes, Alejandro |
| spellingShingle |
Bernal, José Herrero, Miguel Velasco, Diego Elvira, Carlos Cifuentes, Alejandro Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| author_facet |
Bernal, José Herrero, Miguel Velasco, Diego Elvira, Carlos Cifuentes, Alejandro |
| author_sort |
Bernal, José |
| title |
Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| title_short |
Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| title_full |
Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| title_fullStr |
Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| title_full_unstemmed |
Cationic copolymers as physically adsorbed coatings for capillary electrophoresis: Connections between structure and performance |
| title_sort |
cationic copolymers as physically adsorbed coatings for capillary electrophoresis: connections between structure and performance |
| publishDate |
2010 |
| url |
http://hdl.handle.net/10261/113929 |
| work_keys_str_mv |
AT bernaljose cationiccopolymersasphysicallyadsorbedcoatingsforcapillaryelectrophoresisconnectionsbetweenstructureandperformance AT herreromiguel cationiccopolymersasphysicallyadsorbedcoatingsforcapillaryelectrophoresisconnectionsbetweenstructureandperformance AT velascodiego cationiccopolymersasphysicallyadsorbedcoatingsforcapillaryelectrophoresisconnectionsbetweenstructureandperformance AT elviracarlos cationiccopolymersasphysicallyadsorbedcoatingsforcapillaryelectrophoresisconnectionsbetweenstructureandperformance AT cifuentesalejandro cationiccopolymersasphysicallyadsorbedcoatingsforcapillaryelectrophoresisconnectionsbetweenstructureandperformance |
| _version_ |
1777671188573585408 |