Techniques for detecting genetically modified crops and products
The cultivation of genetically modified crops is becoming increasingly important; more traits are emerging and more acres than ever before are being planted with GM varieties. The release of GM crops and products in the markets worldwide has increased the regulatory need to monitor and verify the presence and the amount of GM varieties in crops and products. Labeling legislation and trade requirements differ from one country to another, leading to the necessity for the development of reliable and sensitive analytical methods for detection, identification and quantification of GM varieties in crops and their products. GM crops and their products can be identified by detecting either the inserted genetic material at DNA level, the resulting protein or phenotype. Several analytical methods such as methods based on the polymerase chain reaction (PCR) for detecting the inserted DNA, immunological assays for detecting the resulting protein, or using bioassays to detect the resultant phenotype have been developed. So far only PCR has found broad application in GMO detection as a generally accepted method for regulatory purposes. Presently, real-time PCR can be considered as the most powerful tool for the detection and quantification of GM crops and products.
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Format: | Journal Article biblioteca |
Language: | English |
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African Journals Online
2005
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Subjects: | genetically modified crop, genetic transformation, detection, phenotype, biotechnology, dna, |
Online Access: | https://hdl.handle.net/10568/91849 https://doi.org/10.4314/ajfand.v4i13.71830 |
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dig-cgspace-10568-918492023-12-08T19:36:04Z Techniques for detecting genetically modified crops and products Tripathi, L. genetically modified crop genetic transformation detection phenotype biotechnology dna The cultivation of genetically modified crops is becoming increasingly important; more traits are emerging and more acres than ever before are being planted with GM varieties. The release of GM crops and products in the markets worldwide has increased the regulatory need to monitor and verify the presence and the amount of GM varieties in crops and products. Labeling legislation and trade requirements differ from one country to another, leading to the necessity for the development of reliable and sensitive analytical methods for detection, identification and quantification of GM varieties in crops and their products. GM crops and their products can be identified by detecting either the inserted genetic material at DNA level, the resulting protein or phenotype. Several analytical methods such as methods based on the polymerase chain reaction (PCR) for detecting the inserted DNA, immunological assays for detecting the resulting protein, or using bioassays to detect the resultant phenotype have been developed. So far only PCR has found broad application in GMO detection as a generally accepted method for regulatory purposes. Presently, real-time PCR can be considered as the most powerful tool for the detection and quantification of GM crops and products. 2005 2018-03-23T06:48:53Z 2018-03-23T06:48:53Z Journal Article Tripathi, L. (2005). Techniques for detecting genetically modified crops and products. African Journal of Biotechnology, 4(13), 1472-1479. 1684-5315 https://hdl.handle.net/10568/91849 https://doi.org/10.4314/ajfand.v4i13.71830 en Open Access p. 1472-1479 African Journals Online |
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genetically modified crop genetic transformation detection phenotype biotechnology dna genetically modified crop genetic transformation detection phenotype biotechnology dna Tripathi, L. Techniques for detecting genetically modified crops and products |
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The cultivation of genetically modified crops is becoming increasingly important; more traits are emerging and more acres than ever before are being planted with GM varieties. The release of GM crops and products in the markets worldwide has increased the regulatory need to monitor and verify the presence and the amount of GM varieties in crops and products. Labeling legislation and trade requirements differ from one country to another, leading to the necessity for the development of reliable and sensitive analytical methods for detection, identification and quantification of GM varieties in crops and their products. GM crops and their products can be identified by detecting either the inserted genetic material at DNA level, the resulting protein or phenotype. Several analytical methods such as methods based on the polymerase chain reaction (PCR) for detecting the inserted DNA, immunological assays for detecting the resulting protein, or using bioassays to detect the resultant phenotype have been developed. So far only PCR has found broad application in GMO detection as a generally accepted method for regulatory purposes. Presently, real-time PCR can be considered as the most powerful tool for the detection and quantification of GM crops and products. |
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Journal Article |
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genetically modified crop genetic transformation detection phenotype biotechnology dna |
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Tripathi, L. |
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Tripathi, L. |
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Tripathi, L. |
title |
Techniques for detecting genetically modified crops and products |
title_short |
Techniques for detecting genetically modified crops and products |
title_full |
Techniques for detecting genetically modified crops and products |
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Techniques for detecting genetically modified crops and products |
title_full_unstemmed |
Techniques for detecting genetically modified crops and products |
title_sort |
techniques for detecting genetically modified crops and products |
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African Journals Online |
publishDate |
2005 |
url |
https://hdl.handle.net/10568/91849 https://doi.org/10.4314/ajfand.v4i13.71830 |
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AT tripathil techniquesfordetectinggeneticallymodifiedcropsandproducts |
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1787232045956595712 |