AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America
The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (Phaseolus lunatus L.) and related species (P. augusti Harms, P. bolivianus Piper, P. pachyrrhizoides Harms, and P. rosei Piper) from South America and to identify specific genetic reserves for conservation. These relationships were investigated by means of amplified restriction fragment length polymorphism (AFLPs) on total genomic DNA. The 122 accessions formed a cluster that was distant from common bean (P. vulgaris L.), confirming earlier morphology and hybridology data. Two gene pools of wild Lima beans were confirmed. One was widely distributed in neotropical lowlands, while the other was restricted to the western Andes, in Ecuador and northern Peru. The study also revealed the existence of a third group of wild Lima bean distributed in the Departments of Boyac a ´ and Cundinamarca, Colombia. The three species P. augusti, P. bolivianus, and P. pachyrrhizoides differed very little, certainly not sufficiently to merit a separate taxonomic ranking at the species level. The accessions could be grouped instead according to four geographic origins: Ecuador and northern Peru; Department of Jun ? ´ n, Peru; Departments of Cuzco and Apur ? ´ mac, Peru; and Bolivia and northwestern Argentina. Results from this study should result in a better selection of parental materials in breeding programs and point to areas where germplasm collections and conservation are needed.
Main Authors: | , , , , , |
---|---|
Format: | Journal Article biblioteca |
Language: | English |
Published: |
1999
|
Subjects: | phaseolus lunatus, dna fingerprinting, taxonomy, genetic resources, plant resources, genotypes, huellas genéticas adn, taxonomía, recursos genéticos, recursos vegetales, genotipos, |
Online Access: | https://hdl.handle.net/10568/42559 http://ciat-library.ciat.cgiar.org/articulos_ciat/caicedo.pdf |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-42559 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-425592023-03-14T19:09:12Z AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America Caicedo, AL Gaitán Solís, Eliana Duque E., Myriam Cristina Toro Chica, Orlando Debouck, Daniel G. Tohme, Joseph M. phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (Phaseolus lunatus L.) and related species (P. augusti Harms, P. bolivianus Piper, P. pachyrrhizoides Harms, and P. rosei Piper) from South America and to identify specific genetic reserves for conservation. These relationships were investigated by means of amplified restriction fragment length polymorphism (AFLPs) on total genomic DNA. The 122 accessions formed a cluster that was distant from common bean (P. vulgaris L.), confirming earlier morphology and hybridology data. Two gene pools of wild Lima beans were confirmed. One was widely distributed in neotropical lowlands, while the other was restricted to the western Andes, in Ecuador and northern Peru. The study also revealed the existence of a third group of wild Lima bean distributed in the Departments of Boyac a ´ and Cundinamarca, Colombia. The three species P. augusti, P. bolivianus, and P. pachyrrhizoides differed very little, certainly not sufficiently to merit a separate taxonomic ranking at the species level. The accessions could be grouped instead according to four geographic origins: Ecuador and northern Peru; Department of Jun ? ´ n, Peru; Departments of Cuzco and Apur ? ´ mac, Peru; and Bolivia and northwestern Argentina. Results from this study should result in a better selection of parental materials in breeding programs and point to areas where germplasm collections and conservation are needed. 1999 2014-09-24T07:58:12Z 2014-09-24T07:58:12Z Journal Article 1435-0653 https://hdl.handle.net/10568/42559 http://ciat-library.ciat.cgiar.org/articulos_ciat/caicedo.pdf en Open Access application/pdf Crop Science |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
language |
English |
topic |
phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos |
spellingShingle |
phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos Caicedo, AL Gaitán Solís, Eliana Duque E., Myriam Cristina Toro Chica, Orlando Debouck, Daniel G. Tohme, Joseph M. AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
description |
The taxonomic classification of the wild Lima bean complex needs to be assessed to select species for use in breeding programs and to identify genetic resources for conservation. The objectives of this study were to determine the genetic relationships among, and the phylogeny of, wild Lima bean (Phaseolus lunatus L.) and related species (P. augusti Harms, P. bolivianus Piper, P. pachyrrhizoides Harms, and P. rosei Piper) from South America and to identify specific genetic reserves for conservation. These relationships were investigated by means of amplified restriction fragment length polymorphism (AFLPs) on total genomic DNA. The 122 accessions formed a cluster that was distant from common bean (P. vulgaris L.), confirming earlier morphology and hybridology data. Two gene pools of wild Lima beans were confirmed. One was widely distributed in neotropical lowlands, while the other was restricted to the western Andes, in Ecuador and northern Peru. The study also revealed the existence of a third group of wild Lima bean distributed in the Departments of Boyac a ´ and Cundinamarca, Colombia. The three species P. augusti, P. bolivianus, and P. pachyrrhizoides differed very little, certainly not sufficiently to merit a separate taxonomic ranking at the species level. The accessions could be grouped instead according to four geographic origins: Ecuador and northern Peru; Department of Jun ? ´ n, Peru; Departments of Cuzco and Apur ? ´ mac, Peru; and Bolivia and northwestern Argentina. Results from this study should result in a better selection of parental materials in breeding programs and point to areas where germplasm collections and conservation are needed. |
format |
Journal Article |
topic_facet |
phaseolus lunatus dna fingerprinting taxonomy genetic resources plant resources genotypes huellas genéticas adn taxonomía recursos genéticos recursos vegetales genotipos |
author |
Caicedo, AL Gaitán Solís, Eliana Duque E., Myriam Cristina Toro Chica, Orlando Debouck, Daniel G. Tohme, Joseph M. |
author_facet |
Caicedo, AL Gaitán Solís, Eliana Duque E., Myriam Cristina Toro Chica, Orlando Debouck, Daniel G. Tohme, Joseph M. |
author_sort |
Caicedo, AL |
title |
AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
title_short |
AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
title_full |
AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
title_fullStr |
AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
title_full_unstemmed |
AFLP fingerprinting of Phaseolus lunatus L. and related wild species from South America |
title_sort |
aflp fingerprinting of phaseolus lunatus l. and related wild species from south america |
publishDate |
1999 |
url |
https://hdl.handle.net/10568/42559 http://ciat-library.ciat.cgiar.org/articulos_ciat/caicedo.pdf |
work_keys_str_mv |
AT caicedoal aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica AT gaitansoliseliana aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica AT duqueemyriamcristina aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica AT torochicaorlando aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica AT debouckdanielg aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica AT tohmejosephm aflpfingerprintingofphaseoluslunatuslandrelatedwildspeciesfromsouthamerica |
_version_ |
1779050034596151296 |