Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007
Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with restriction analysis. None of the sera collected from pigs with clinical symptoms were positive using the OIE serological prescribed tests. However, seven haemadsorbing viruses were isolated in macrophage culture and genotyped by partial p72 and full length p54-gene sequencing. Four of these viruses were isolated directly from serum samples. All the viruses were classified within the domesticpig cycle-associated p72 and p54 genotype IX which also includes viruses responsible for ASF outbreaks in Kenya in 2006 and 2007 and Uganda in 2003. To define virus relationships at higher resolution, typing was performed by analysis of tetrameric amino acid repeat regions within the central variable region (CVR) of the B602L gene. Ugandan isolates sequences exhibited 100% identity to viruses isolated from outbreaks in Kenya in 2007. The identity was greater than the viruses obtained from an earlier outbreak in Kenya in 2006. This provides further evidence that genetically similar ASFV virus within p72 Genotype IX may be circulating between Kenya and Uganda.
Main Authors: | , , , , , , , |
---|---|
Format: | Journal Article biblioteca |
Published: |
2011
|
Subjects: | animal diseases, livestock, swine, |
Online Access: | https://hdl.handle.net/10568/35253 http://www.ajol.info/index.php/ajb/article/view/93420 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-cgspace-10568-35253 |
---|---|
record_format |
koha |
spelling |
dig-cgspace-10568-352532023-02-15T10:17:09Z Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 Gallardo, C. Ademun, A.R. Nieto, R. Nantima, Noelina Arias, Marisa Martin, E. Pelayo, V. Bishop, Richard P. animal diseases livestock swine Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with restriction analysis. None of the sera collected from pigs with clinical symptoms were positive using the OIE serological prescribed tests. However, seven haemadsorbing viruses were isolated in macrophage culture and genotyped by partial p72 and full length p54-gene sequencing. Four of these viruses were isolated directly from serum samples. All the viruses were classified within the domesticpig cycle-associated p72 and p54 genotype IX which also includes viruses responsible for ASF outbreaks in Kenya in 2006 and 2007 and Uganda in 2003. To define virus relationships at higher resolution, typing was performed by analysis of tetrameric amino acid repeat regions within the central variable region (CVR) of the B602L gene. Ugandan isolates sequences exhibited 100% identity to viruses isolated from outbreaks in Kenya in 2007. The identity was greater than the viruses obtained from an earlier outbreak in Kenya in 2006. This provides further evidence that genetically similar ASFV virus within p72 Genotype IX may be circulating between Kenya and Uganda. 2011 2014-04-14T10:55:39Z 2014-04-14T10:55:39Z Journal Article Gallardo, C., Ademun, A.R., Nieto, R., Nantima, N., Arias, M., Martin, E., Pelayo, V. and Bishop, R.P. 2011. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007. African Journal of Biotechnology 10(17): 3488 - 3497 1684-5315 https://hdl.handle.net/10568/35253 http://www.ajol.info/index.php/ajb/article/view/93420 Open Access African Journal of Biotechnology |
institution |
CGIAR |
collection |
DSpace |
country |
Francia |
countrycode |
FR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-cgspace |
tag |
biblioteca |
region |
Europa del Oeste |
libraryname |
Biblioteca del CGIAR |
topic |
animal diseases livestock swine animal diseases livestock swine |
spellingShingle |
animal diseases livestock swine animal diseases livestock swine Gallardo, C. Ademun, A.R. Nieto, R. Nantima, Noelina Arias, Marisa Martin, E. Pelayo, V. Bishop, Richard P. Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
description |
Samples from infected domestic pigs associated with an outbreak of African swine fever (ASF) in three districts of central Uganda in 2007 were confirmed as being infected with African swine fever virus (ASFV) using a P72 gene-based polymerase chain reaction amplification (PCR) assay combined with restriction analysis. None of the sera collected from pigs with clinical symptoms were positive using the OIE serological prescribed tests. However, seven haemadsorbing viruses were isolated in macrophage culture and genotyped by partial p72 and full length p54-gene sequencing. Four of these viruses were isolated directly from serum samples. All the viruses were classified within the domesticpig cycle-associated p72 and p54 genotype IX which also includes viruses responsible for ASF outbreaks in Kenya in 2006 and 2007 and Uganda in 2003. To define virus relationships at higher resolution, typing was performed by analysis of tetrameric amino acid repeat regions within the central variable region (CVR) of the B602L gene. Ugandan isolates sequences exhibited 100% identity to viruses isolated from outbreaks in Kenya in 2007. The identity was greater than the viruses obtained from an earlier outbreak in Kenya in 2006. This provides further evidence that genetically similar ASFV virus within p72 Genotype IX may be circulating between Kenya and Uganda. |
format |
Journal Article |
topic_facet |
animal diseases livestock swine |
author |
Gallardo, C. Ademun, A.R. Nieto, R. Nantima, Noelina Arias, Marisa Martin, E. Pelayo, V. Bishop, Richard P. |
author_facet |
Gallardo, C. Ademun, A.R. Nieto, R. Nantima, Noelina Arias, Marisa Martin, E. Pelayo, V. Bishop, Richard P. |
author_sort |
Gallardo, C. |
title |
Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
title_short |
Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
title_full |
Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
title_fullStr |
Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
title_full_unstemmed |
Genotyping of African swine fever virus (ASFV) isolates associated with disease outbreaks in Uganda in 2007 |
title_sort |
genotyping of african swine fever virus (asfv) isolates associated with disease outbreaks in uganda in 2007 |
publishDate |
2011 |
url |
https://hdl.handle.net/10568/35253 http://www.ajol.info/index.php/ajb/article/view/93420 |
work_keys_str_mv |
AT gallardoc genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT ademunar genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT nietor genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT nantimanoelina genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT ariasmarisa genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT martine genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT pelayov genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 AT bishoprichardp genotypingofafricanswinefevervirusasfvisolatesassociatedwithdiseaseoutbreaksinugandain2007 |
_version_ |
1779065460872970240 |