Molecular identification of viral pathogens in SPF (Specific pathogen free) shrimp

After the outbreak of various viral diseases, specifically white spot syndrome (WSS) in farmed Litopenaeus vannamei in the past few years, this non-native shrimp species in Iran requires a generator-builder with specific a pathogen-free approach. This Research was done by the Iran Shrimp Research Center (ISRC) to access accurate information regarding specific pathogen-free shrimp production and avoid using shrimp broodstock production through foreign investors. This research was part of the molecular viral study. For this trial, selected shrimps in farms were sampled and screened for main viruses (OIE list). Shrimps without infection were brought to the quarantine system for one month and at the end of the quarantine they were screened again. In addition, in winter spending, before and after spawning, it’s offspring in F1 and F2 were also screened for viral pathogens by IQ2000 kit at the Iran Shrimp Research Center lab and Iranian veterinary organization lab. All shrimp products such as fresh and consumed food were tested for OIE list at all stages during project performance and the result of tests were negative. Sequenced and molecular tests with specific primers were used to determine the presence of infected samples carrying the virus in any of the products, such as shrimp and consumed food, the infected samples. The result of all the tests during last three years, shows that all the shrimp and their food products were pathogen-free and safe to use.

Bibliographic Details

Main Authors:	Yeganeh, V., Zorieh Zahra, S.J., Mirbakhsh, M., Ghaednia, B., Dashtiannasab, A., Nazari, M.A., Keshtkar, E., Mehrabi, M.R., Bnadrakhshan, R., Ansari, F., Mohmmadi, E., Ranjbari, Zh., Shabani, M.J., Ghanatian, H., Jamali, Gh., Afsharnasab, M.
Format:	monograph biblioteca
Language:	Persian
Published:	Iranian Fisheries Science Research Institute 2016
Subjects:	Biology, Iran, Shrim, SPF, Virus, White Spot Syndrome Virus, WSS, Spawning, Molecular,
Online Access:	http://hdl.handle.net/1834/40141
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