Production of triploid African catfish, Clarias gariepinus (Burchell), using chromosome manipulation techniques

An experiment was conducted to induce triploidy in African catfish, Clarias gariepinus,using heat shock and cold shock techniques. Cold shock at a temperature of 0± 1°C and5±1°C for a duration of 15, 30, 45 and 60 min and heat shock at a temperature of40±0.5°C and 41 ±OS C for a duration of 1, 2 and 3 min was given to induce triploidy 5min after fertilization. Maximum percentage of triploids (91.4%) were obtained in theheat shock at a temperature of 40±0SC for a duration of 1 min whereas cold shock at0± 1 C for a duration of 60 min yielded 90% of triploids. Chromosome analysis revealedthat diploids have 54 chromosomes and triploids have 81 chromosomes. The erythrocytemeasurements of the minor axis and major axis were 1.17 times larger in treated fishthan in controls. The growth studies showed that the growth rate was not significantlyaffected in triploids.

Bibliographic Details

Main Authors:	Marx, K.K., Sukumaran, N.
Format:	article biblioteca
Language:	English
Published:	2007
Subjects:	Aquaculture, Fisheries, Triploid, Chromosome manipulation, Clarias gariepinus,
Online Access:	http://hdl.handle.net/1834/33158
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