Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer

The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged.

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Main Authors: Ghiasi, Maryam, Pourgholam, R., Nasrolahzadeh, H., Saeedi, A.A., Safari, R., Zahedi, A., Binaii, M., Nasiri, S.F., Razeghian, Gh.R.
Format: Report biblioteca
Language:Persian
Published: Iranian Fisheries Science Research Institute 2014
Subjects:Chemical, Physico-chemical, Pathogenic, Bacteria, Cyprinid, Cow dung, Fertilizer, Microbial flora, Cyprinus fish, Dissolved oxygen, Transparency, Alkalinity, Temperature, pH, Kidney, Pseudomonas, E.coli,
Online Access:http://hdl.handle.net/1834/13549
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spelling dig-aquadocs-1834-135492021-05-19T06:47:51Z Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer Ghiasi, Maryam Pourgholam, R. Nasrolahzadeh, H. Saeedi, A.A. Safari, R. Zahedi, A. Binaii, M. Nasiri, S.F. Razeghian, Gh.R. Chemical Physico-chemical Pathogenic Bacteria Cyprinid Cow dung Fertilizer Microbial flora Cyprinus fish Dissolved oxygen Transparency Alkalinity Temperature pH Kidney Pseudomonas E.coli The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged. Iranian Fisheries Science Research Institute Published 2018-08-03T19:32:42Z 2018-08-03T19:32:42Z 2014 Report Refereed http://hdl.handle.net/1834/13549 fa 46309; 47pp. Iran Iranian Fisheries Science Research Institute Tehran, Iran
institution UNESCO
collection DSpace
country Francia
countrycode FR
component Bibliográfico
access En linea
databasecode dig-aquadocs
tag biblioteca
region Europa del Oeste
libraryname Repositorio AQUADOCS
language Persian
topic Chemical
Physico-chemical
Pathogenic
Bacteria
Cyprinid
Cow dung
Fertilizer
Microbial flora
Cyprinus fish
Dissolved oxygen
Transparency
Alkalinity
Temperature
pH
Kidney
Pseudomonas
E.coli
Chemical
Physico-chemical
Pathogenic
Bacteria
Cyprinid
Cow dung
Fertilizer
Microbial flora
Cyprinus fish
Dissolved oxygen
Transparency
Alkalinity
Temperature
pH
Kidney
Pseudomonas
E.coli
spellingShingle Chemical
Physico-chemical
Pathogenic
Bacteria
Cyprinid
Cow dung
Fertilizer
Microbial flora
Cyprinus fish
Dissolved oxygen
Transparency
Alkalinity
Temperature
pH
Kidney
Pseudomonas
E.coli
Chemical
Physico-chemical
Pathogenic
Bacteria
Cyprinid
Cow dung
Fertilizer
Microbial flora
Cyprinus fish
Dissolved oxygen
Transparency
Alkalinity
Temperature
pH
Kidney
Pseudomonas
E.coli
Ghiasi, Maryam
Pourgholam, R.
Nasrolahzadeh, H.
Saeedi, A.A.
Safari, R.
Zahedi, A.
Binaii, M.
Nasiri, S.F.
Razeghian, Gh.R.
Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
description The aim of this study was comparison the effect of cow dung as organic fertilizer and chemical fertilizer on microbial flora, fish and human pathogenic bacteria and physico-chemical parameters of cyprinus fish pond water. The water samples were collected from two ponds, pond 1 was fertilized by cow dung and pond 2 was fertilized by chemical fertilizer, respectively from May to October 2011. . The aerobic and anaerobic bacteria were enumerated in TSA by serial dilution of the sample, followed by conventional pure plate method. Coliforms bacteria were similarly isolated on Chrom agarTM ECC. Moreover, the water samples were used to analysis of BOD, dissolved oxygen, conductivity, transparency, total alkalinity, total dissolved solid, total hardness, total nitrogen and total phosphorous. Temperature and pH were measured by thermometer and portable pH meter in place of ponds. For isolating of fish and human pathogenic bacteria, thirty six fish were collected from pond 1 from July to October and then sampled from their liver and kidney and inoculated in blood agar. The mean of total count of bacteria and total coliform count in cow dung sample were 4 × 104 ± 1200 and 4700 ± 300 CFUmL-1 respectively. The mean of water total count bacteria of pond 1 were significantly higher than the water of pond 2. The water total count bacteria of pond 1 were significantly increased in August, but water total count of pond 2 was not shown significant variation from May to October. The results showed that Pseudomonas, Klebsiella, Enterobacter, Yersinia and E.coli were isolated from water of pond 1 but just Pseudomonas and E.coli were isolated from water of pond 2. Moreover, the human pathogenic bacteria shuc as Shigella and Salmonella were not isolated from of water and fish samples from pond 1. In this study, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. The total hardness, total phosphorus, total dissolved solid and conductivity of the pond 1 water were significantly higher than the pond 2 water. But the BOD, dissolved oxygen and transparency of the pond 2 were significantly higher than the pond 1 water. There were no significant differences in the total nitrogen, total alkalinity and pH between water of pond 1 and 2. In recent years, the chemical fertilizer has been used in cyprinus fish pond but many studies have showed that they were high risked as environmental pollution. Thus, the use of livestock manure especially cow dung could be a suitable alternative. The results showed the use of cow dung increased the bacterial population and diversity. Increasing of bacteria population caused increased total phosphorus that plays an important role in bloom of plankton because we found decreasing transparency and planktons are an important food for cyprinus fish. In despite of isolation of fish and human pathogenic bacteria from water, the bacteria growths were negative in all of the inoculated plate from fish liver and kidney. In summary, it seems the cow dung is a suitable alternative for fertilizing of cyprinus fish pond water if a microbial and physico-chemical monitoring of fish and pond water were continuously arranged.
format Report
topic_facet Chemical
Physico-chemical
Pathogenic
Bacteria
Cyprinid
Cow dung
Fertilizer
Microbial flora
Cyprinus fish
Dissolved oxygen
Transparency
Alkalinity
Temperature
pH
Kidney
Pseudomonas
E.coli
author Ghiasi, Maryam
Pourgholam, R.
Nasrolahzadeh, H.
Saeedi, A.A.
Safari, R.
Zahedi, A.
Binaii, M.
Nasiri, S.F.
Razeghian, Gh.R.
author_facet Ghiasi, Maryam
Pourgholam, R.
Nasrolahzadeh, H.
Saeedi, A.A.
Safari, R.
Zahedi, A.
Binaii, M.
Nasiri, S.F.
Razeghian, Gh.R.
author_sort Ghiasi, Maryam
title Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
title_short Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
title_full Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
title_fullStr Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
title_full_unstemmed Quantity Evaluation and Identification of Pathogenic Bacteria in Water Pond and Cultivated Cyprinid Fish Enrichment by Natural(Cow dong) and Chemical fertilizer
title_sort quantity evaluation and identification of pathogenic bacteria in water pond and cultivated cyprinid fish enrichment by natural(cow dong) and chemical fertilizer
publisher Iranian Fisheries Science Research Institute
publishDate 2014
url http://hdl.handle.net/1834/13549
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