Correlations between ovarian follicular blood flow and superovulatory responses in ewes.
Abstract: The primary goal of this study was to employ ultrasonography to examine the ovaries of ewes undergoing superovulatory treatment for correlations between antral follicular blood flow and ovarian responses/embryo yields. Five Santa Inês ewes were subjected to a short- (Days 0?6, Group 1) and five to a long-term progesterone-based protocol (Days 0?12, Group 2) to synchronize estrus and ovulations after the superovulatory treatment. Porcine FSH (pFSH, 200 mg) was administered in 8 decreasing doses over 4 days, starting on Days 4 and 10 in Groups 1 and 2, respectively. After CIDR removal, all ewes were bred by a ram and embryos were recovered surgically 7 days later. Transrectal ovarian ultrasonography was performed the day before and on all 4 days of the superovulatory treatment. Both an arbitrary-scale [(0) non-detectable; (1) small; (2) moderate; (3) intense blood flow] and quantitative analysis of the blood flow area were used to assess the follicular blood flow in color Doppler images. There were no significant correlations between the arbitrary blood flow scores and superovulatory responses in the ewes of the present study. However, there was a positive correlation between the quantitative estimates of follicular blood flow on the final day of the superovulatory treatment, and the number (DA: r = 0.68, P < 0.05; DA/TA × 100%: r = 0.85, P < 0.05) and percentage (DA: r = 0.65, P < 0.05; DA/TA × 100%: r = 0.91, P < 0.001) of unfertilized eggs (DA: Doppler area, TA: total area of the largest ovarian cross section). This experiment presents a commercially practical tool for predicting superovulatory outcomes in ewes and evidence for the existence of follicular blood flow threshold that may impinge negatively on oocyte quality when surpassed during hormonal ovarian superstimulation.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2014-10-24
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Subjects: | Color Doppler sonography; Antral follicles, Ovino, Ovelha, Reprodução animal, Ovário, Superovulação, Sheep, Ewes, Superovulation, Blood circulation, Reproduction, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/998372 |
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dig-alice-doc-9983722017-08-16T01:58:40Z Correlations between ovarian follicular blood flow and superovulatory responses in ewes. OLIVEIRA, M. E. F. FELICIANO, M. A. R. D'AMATO, C. C. OLIVEIRA, L. G. BICUDO, S. D. FONSECA, J. F. da VICENTE, W. R. R. VISCO, E. BARTLEWSKID, P. M. Maria E. F. Oliveira, São Paulo State University (USP) - Jaboticabal, SP, Brazil; Marcus A. R. Feliciano, USP - Jaboticabal, SP, Brazil; Carla C. D'Amato, USP - Jaboticabal, SP, Brazil; Luís G. Oliveira, USP - Jaboticabal, SP, Brazil; Sony D. Bicudo, Paulo State University (USP) - Botucatu, SP, Brazil; Jeferson Ferreira da Fonseca, CNPC; Wilter R. R. Vicente, USP - Jaboticabal, SP, Brazil; Elise Visco, University of Guelph, Guelph, Canada; Pawel M. Bartlewskid, University of Guelph, Guelph, Canada. Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction Abstract: The primary goal of this study was to employ ultrasonography to examine the ovaries of ewes undergoing superovulatory treatment for correlations between antral follicular blood flow and ovarian responses/embryo yields. Five Santa Inês ewes were subjected to a short- (Days 0?6, Group 1) and five to a long-term progesterone-based protocol (Days 0?12, Group 2) to synchronize estrus and ovulations after the superovulatory treatment. Porcine FSH (pFSH, 200 mg) was administered in 8 decreasing doses over 4 days, starting on Days 4 and 10 in Groups 1 and 2, respectively. After CIDR removal, all ewes were bred by a ram and embryos were recovered surgically 7 days later. Transrectal ovarian ultrasonography was performed the day before and on all 4 days of the superovulatory treatment. Both an arbitrary-scale [(0) non-detectable; (1) small; (2) moderate; (3) intense blood flow] and quantitative analysis of the blood flow area were used to assess the follicular blood flow in color Doppler images. There were no significant correlations between the arbitrary blood flow scores and superovulatory responses in the ewes of the present study. However, there was a positive correlation between the quantitative estimates of follicular blood flow on the final day of the superovulatory treatment, and the number (DA: r = 0.68, P < 0.05; DA/TA × 100%: r = 0.85, P < 0.05) and percentage (DA: r = 0.65, P < 0.05; DA/TA × 100%: r = 0.91, P < 0.001) of unfertilized eggs (DA: Doppler area, TA: total area of the largest ovarian cross section). This experiment presents a commercially practical tool for predicting superovulatory outcomes in ewes and evidence for the existence of follicular blood flow threshold that may impinge negatively on oocyte quality when surpassed during hormonal ovarian superstimulation. 2014-10-24T11:11:11Z 2014-10-24T11:11:11Z 2014-10-24 2014 2014-10-27T11:11:11Z Artigo de periódico Animal Reproduction Science, Champaign, v. 144, n. 1/2, p. 30-37, Jan. 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/998372 en eng openAccess |
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Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction |
spellingShingle |
Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction OLIVEIRA, M. E. F. FELICIANO, M. A. R. D'AMATO, C. C. OLIVEIRA, L. G. BICUDO, S. D. FONSECA, J. F. da VICENTE, W. R. R. VISCO, E. BARTLEWSKID, P. M. Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
description |
Abstract: The primary goal of this study was to employ ultrasonography to examine the ovaries of ewes undergoing superovulatory treatment for correlations between antral follicular blood flow and ovarian responses/embryo yields. Five Santa Inês ewes were subjected to a short- (Days 0?6, Group 1) and five to a long-term progesterone-based protocol (Days 0?12, Group 2) to synchronize estrus and ovulations after the superovulatory treatment. Porcine FSH (pFSH, 200 mg) was administered in 8 decreasing doses over 4 days, starting on Days 4 and 10 in Groups 1 and 2, respectively. After CIDR removal, all ewes were bred by a ram and embryos were recovered surgically 7 days later. Transrectal ovarian ultrasonography was performed the day before and on all 4 days of the superovulatory treatment. Both an arbitrary-scale [(0) non-detectable; (1) small; (2) moderate; (3) intense blood flow] and quantitative analysis of the blood flow area were used to assess the follicular blood flow in color Doppler images. There were no significant correlations between the arbitrary blood flow scores and superovulatory responses in the ewes of the present study. However, there was a positive correlation between the quantitative estimates of follicular blood flow on the final day of the superovulatory treatment, and the number (DA: r = 0.68, P < 0.05; DA/TA × 100%: r = 0.85, P < 0.05) and percentage (DA: r = 0.65, P < 0.05; DA/TA × 100%: r = 0.91, P < 0.001) of unfertilized eggs (DA: Doppler area, TA: total area of the largest ovarian cross section). This experiment presents a commercially practical tool for predicting superovulatory outcomes in ewes and evidence for the existence of follicular blood flow threshold that may impinge negatively on oocyte quality when surpassed during hormonal ovarian superstimulation. |
author2 |
Maria E. F. Oliveira, São Paulo State University (USP) - Jaboticabal, SP, Brazil; Marcus A. R. Feliciano, USP - Jaboticabal, SP, Brazil; Carla C. D'Amato, USP - Jaboticabal, SP, Brazil; Luís G. Oliveira, USP - Jaboticabal, SP, Brazil; Sony D. Bicudo, Paulo State University (USP) - Botucatu, SP, Brazil; Jeferson Ferreira da Fonseca, CNPC; Wilter R. R. Vicente, USP - Jaboticabal, SP, Brazil; Elise Visco, University of Guelph, Guelph, Canada; Pawel M. Bartlewskid, University of Guelph, Guelph, Canada. |
author_facet |
Maria E. F. Oliveira, São Paulo State University (USP) - Jaboticabal, SP, Brazil; Marcus A. R. Feliciano, USP - Jaboticabal, SP, Brazil; Carla C. D'Amato, USP - Jaboticabal, SP, Brazil; Luís G. Oliveira, USP - Jaboticabal, SP, Brazil; Sony D. Bicudo, Paulo State University (USP) - Botucatu, SP, Brazil; Jeferson Ferreira da Fonseca, CNPC; Wilter R. R. Vicente, USP - Jaboticabal, SP, Brazil; Elise Visco, University of Guelph, Guelph, Canada; Pawel M. Bartlewskid, University of Guelph, Guelph, Canada. OLIVEIRA, M. E. F. FELICIANO, M. A. R. D'AMATO, C. C. OLIVEIRA, L. G. BICUDO, S. D. FONSECA, J. F. da VICENTE, W. R. R. VISCO, E. BARTLEWSKID, P. M. |
format |
Artigo de periódico |
topic_facet |
Color Doppler sonography; Antral follicles Ovino Ovelha Reprodução animal Ovário Superovulação Sheep Ewes Superovulation Blood circulation Reproduction |
author |
OLIVEIRA, M. E. F. FELICIANO, M. A. R. D'AMATO, C. C. OLIVEIRA, L. G. BICUDO, S. D. FONSECA, J. F. da VICENTE, W. R. R. VISCO, E. BARTLEWSKID, P. M. |
author_sort |
OLIVEIRA, M. E. F. |
title |
Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
title_short |
Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
title_full |
Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
title_fullStr |
Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
title_full_unstemmed |
Correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
title_sort |
correlations between ovarian follicular blood flow and superovulatory responses in ewes. |
publishDate |
2014-10-24 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/998372 |
work_keys_str_mv |
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