Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley.
The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 x 105 cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production.
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Format: | Separatas biblioteca |
Language: | English eng |
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2014-10-22
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Subjects: | Brazilian wines, Levedura., Viticultura, Uva, Fermentação, DNA, Saccharomyces Cerevisiae., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/998012 |
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dig-alice-doc-9980122017-08-16T00:58:58Z Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. PONZZES-GOMES, C. M. de MÉLO, D. L. de SANTANA, C. A. PEREIRA, G. E. MENDONÇA, M. O. C. GOMES, F. C. O. OLIVEIRA, E. S. BARBOSA JR, A. M. TRINDADE, R. C. ROSA, C. A. GIULIANO ELIAS PEREIRA, CNPUV. Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 x 105 cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production. 2014-10-22T11:11:11Z 2014-10-22T11:11:11Z 2014-10-22 2014 2019-04-02T11:11:11Z Separatas Brazilian Journal of Microbiology, v. 45, n. 2, p. 411-416, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/998012 en eng openAccess |
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Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. |
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Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. PONZZES-GOMES, C. M. de MÉLO, D. L. de SANTANA, C. A. PEREIRA, G. E. MENDONÇA, M. O. C. GOMES, F. C. O. OLIVEIRA, E. S. BARBOSA JR, A. M. TRINDADE, R. C. ROSA, C. A. Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
description |
The aims of this work was to characterise indigenous Saccharomyces cerevisiae strains in the naturally fermented juice of grape varieties Cabernet Sauvignon, Grenache, Tempranillo, Sauvignon Blanc and Verdejo used in the São Francisco River Valley, northeastern Brazil. In this study, 155 S. cerevisiae and 60 non-Saccharomyces yeasts were isolated and identified using physiological tests and sequencing of the D1/D2 domains of the large subunit of the rRNA gene. Among the non-Saccharomyces species, Rhodotorula mucilaginosa was the most common species, followed by Pichia kudriavzevii, Candida parapsilosis, Meyerozyma guilliermondii, Wickerhamomyces anomalus, Kloeckera apis, P. manshurica, C. orthopsilosis and C. zemplinina. The population counts of these yeasts ranged among 1.0 to 19 x 105 cfu/mL. A total of 155 isolates of S. cerevisiae were compared by mitochondrial DNA restriction analysis, and five molecular mitochondrial DNA restriction profiles were detected. Indigenous strains of S. cerevisiae isolated from grapes of the São Francisco Valley can be further tested as potential starters for wine production. |
author2 |
GIULIANO ELIAS PEREIRA, CNPUV. |
author_facet |
GIULIANO ELIAS PEREIRA, CNPUV. PONZZES-GOMES, C. M. de MÉLO, D. L. de SANTANA, C. A. PEREIRA, G. E. MENDONÇA, M. O. C. GOMES, F. C. O. OLIVEIRA, E. S. BARBOSA JR, A. M. TRINDADE, R. C. ROSA, C. A. |
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Separatas |
topic_facet |
Brazilian wines Levedura. Viticultura Uva Fermentação DNA Saccharomyces Cerevisiae. |
author |
PONZZES-GOMES, C. M. de MÉLO, D. L. de SANTANA, C. A. PEREIRA, G. E. MENDONÇA, M. O. C. GOMES, F. C. O. OLIVEIRA, E. S. BARBOSA JR, A. M. TRINDADE, R. C. ROSA, C. A. |
author_sort |
PONZZES-GOMES, C. M. de |
title |
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
title_short |
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
title_full |
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
title_fullStr |
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
title_full_unstemmed |
Saccharomyces cerevisiae and non-Saccharomyces yeasts in grape varieties of the São Francisco Valley. |
title_sort |
saccharomyces cerevisiae and non-saccharomyces yeasts in grape varieties of the são francisco valley. |
publishDate |
2014-10-22 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/998012 |
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