Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.

PEREIRA, J. F.; ALMEIDA, A. P. M. M.; COTA, J.; PAMPHILE, J. A.; SILVA, G. F. da; ARAÚJO, E. F. de; GRAMACHO, K. P.; BROMMONSCHENKEL, S. H.; PEREIRA, G. A. G.; QUEIROZ, M. V. de; JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA.

Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.

Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.

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Bibliographic Details
Main Authors:	PEREIRA, J. F., ALMEIDA, A. P. M. M., COTA, J., PAMPHILE, J. A., SILVA, G. F. da, ARAÚJO, E. F. de, GRAMACHO, K. P., BROMMONSCHENKEL, S. H., PEREIRA, G. A. G., QUEIROZ, M. V. de
Other Authors:	JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA.
Format:	Separatas biblioteca
Language:	English eng
Published:	2013-01-23
Subjects:	Moniliophthora perniciosa.,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
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id	dig-alice-doc-946075
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spelling	dig-alice-doc-9460752017-08-15T23:56:21Z Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus. PEREIRA, J. F. ALMEIDA, A. P. M. M. COTA, J. PAMPHILE, J. A. SILVA, G. F. da ARAÚJO, E. F. de GRAMACHO, K. P. BROMMONSCHENKEL, S. H. PEREIRA, G. A. G. QUEIROZ, M. V. de JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA. Moniliophthora perniciosa. Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus. 2013-01-23T11:11:11Z 2013-01-23T11:11:11Z 2013-01-23 2013 2013-06-24T11:11:11Z Separatas Microbiology, v. 159, p. 112-125, 2013. http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075 10.1099/mic.0.062901-0 en eng openAccess
institution	EMBRAPA
collection	DSpace
country	Brasil
countrycode	BR
component	Bibliográfico
access	En linea
databasecode	dig-alice
tag	biblioteca
region	America del Sur
libraryname	Sistema de bibliotecas de EMBRAPA
language	English eng
topic	Moniliophthora perniciosa. Moniliophthora perniciosa.
spellingShingle	Moniliophthora perniciosa. Moniliophthora perniciosa. PEREIRA, J. F. ALMEIDA, A. P. M. M. COTA, J. PAMPHILE, J. A. SILVA, G. F. da ARAÚJO, E. F. de GRAMACHO, K. P. BROMMONSCHENKEL, S. H. PEREIRA, G. A. G. QUEIROZ, M. V. de Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
description	Boto, a class II transposable element, was characterized in the Moniliophthora perniciosa genome. The Boto transposase is highly similar to plant PIF-like transposases that belong to the newest class II superfamily known as PIF/Harbinger. Although Boto shares characteristics with PIF-like elements, other characteristics, such as the transposase intron position, the position and direction of the second ORF, and the footprint, indicate that Boto belongs to a novel family of the PIF/Harbinger superfamily. Southern blot analyses detected 6?12 copies of Boto in C-biotype isolates and a ubiquitous presence among the C- and S-biotypes, as well as a separation in the C-biotype isolates from Bahia State in Brazil in at least two genotypic groups, and a new insertion in the genome of a C-biotype isolate maintained in the laboratory for 6 years. In addition to PCR amplification from a specific insertion site, changes in the Boto hybridization profile after the M. perniciosa sexual cycle and detection of Boto transcripts gave further evidence of Boto activity. As an active family in the genome of M. perniciosa, Boto elements may contribute to genetic variability in this homothallic fungus. This is the first report of a PIF/Harbinger transposon in the genome of a phytopathogenic fungus.
author2	JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA.
author_facet	JORGE FERNANDO PEREIRA, UNIVERSIDADE FEDERAL DE VIÇOSA; ANA PAULA MORAIS MARTINS ALMEIDA, UNIVERSIDADE FEDERAL DE VIÇOSA; JÚNIO COTA, UNIVERSIDADE FEDERAL DE VIÇOSA; JOÃO ALENCAR PAMPHILE, UNIVERSIDADE FEDERAL DE VIÇOSA; GILVAN FERREIRA DA SILVA, CPAA; ELZA FERNANDES DE ARAÚJO, UNIVERSIDADE FEDERAL DE VIÇOSA; KARINA PERES GRAMACHO, CEPLAC; SÉRGIO HERMÍNIO BROMMONSCHENKEL, UNIVERSIDADE FEDERAL DE VIÇOSA; GONÇALO AMARANTE GUIMARÃES PEREIRA, UNIVERSIDADE ESTADUAL DE CAMPINAS; MARISA VIEIRA DE QUEIROZ, UNIVERSIDADE FEDERAL DE VIÇOSA. PEREIRA, J. F. ALMEIDA, A. P. M. M. COTA, J. PAMPHILE, J. A. SILVA, G. F. da ARAÚJO, E. F. de GRAMACHO, K. P. BROMMONSCHENKEL, S. H. PEREIRA, G. A. G. QUEIROZ, M. V. de
format	Separatas
topic_facet	Moniliophthora perniciosa.
author	PEREIRA, J. F. ALMEIDA, A. P. M. M. COTA, J. PAMPHILE, J. A. SILVA, G. F. da ARAÚJO, E. F. de GRAMACHO, K. P. BROMMONSCHENKEL, S. H. PEREIRA, G. A. G. QUEIROZ, M. V. de
author_sort	PEREIRA, J. F.
title	Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_short	Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_full	Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_fullStr	Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_full_unstemmed	Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.
title_sort	boto, a class ii transposon in moniliophthora perniciosa, is the first representative of the pif/harbinger superfamily in a phytopathogenic fungus.
publishDate	2013-01-23
url	http://www.alice.cnptia.embrapa.br/alice/handle/doc/946075
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Boto, a class II transposon in Moniliophthora perniciosa, is the first representative of the PIF/Harbinger superfamily in a phytopathogenic fungus.

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