Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.).
Endophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2009-08-05
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Subjects: | Endophytíc bacteria, Bacillus, Sweet corn, SDS-PAGE, rDNA sequencing, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/488802 |
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dig-alice-doc-4888022017-08-15T21:31:02Z Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). FIGUEIREDO, J. E. F. GOMES, E. A. GUIMARAES, C. T. LANA, U. G. de P. TEIXEIRA, M. A. LIMA, G. V. C. BRESSAN, W. JOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS. Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing Endophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies. 2011-04-10T11:11:11Z 2011-04-10T11:11:11Z 2009-08-05 2009 2018-05-30T11:11:11Z Artigo de periódico Brazilian Journal of Microbiology, São Paulo, v. 40, n. 3, p. 522-534, 2009. http://www.alice.cnptia.embrapa.br/alice/handle/doc/488802 10.1590/S1517-83822009000300014 en eng openAccess |
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Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing |
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Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing FIGUEIREDO, J. E. F. GOMES, E. A. GUIMARAES, C. T. LANA, U. G. de P. TEIXEIRA, M. A. LIMA, G. V. C. BRESSAN, W. Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
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Endophytíc bacteria play an important role in agriculture by improving plant performance and adaptation against biotic and abiotic stresses. In the present study molecular methods were used for identifying Bacillus endophytic bacteria isolated from Brazilian sweet corn. SDS-PAGE of whole-cell protein extract of forty-two isolates revealed a high number of scrutinable bands. Twenty-four isolates were identified in nine different groups of duplicated bacteria and eighteen were identified as unique. Some high-accumulated polipeptides with variable length were observed in almost isolates. Partial sequencing of 16S ribosonal gene revealed that all isolates are Bacillus sp. and among thirteen isolates with similar protein profiles, two were different strains. Among the forty-two isolates identified by rDNA sequencing. Bacillus. subitilis and B. pumilus were the most frequenty species (15 and 12 isolates. respectively) followed by B. licheniforms (7 isolates), B. cereus (5 isolates) and B. uniloliquefascelns (3 isolates). According to present results. SDS-P AGE techínique could be used as a fast and cheap first tool for identifying inter-specific variation in maize endophytic bacterial collections while rDNA sequencing could be applied for analyzing intra-specific variation among isolates with similar protein profile as well as for taxonomic studies. |
author2 |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS. |
author_facet |
JOSE EDSON FONTES FIGUEIREDO, CNPMS; ELIANE APARECIDA GOMES, CNPMS; CLAUDIA TEIXEIRA GUIMARAES, CNPMS; UBIRACI GOMES DE PAULA LANA, CNPMS; Maria Aparecida Teixeira, CNPMS; Guilherme Vitor Correa Lima, Fundação Educacional Monsenhor Messias; WELLINGTON BRESSAN, CNPMS. FIGUEIREDO, J. E. F. GOMES, E. A. GUIMARAES, C. T. LANA, U. G. de P. TEIXEIRA, M. A. LIMA, G. V. C. BRESSAN, W. |
format |
Artigo de periódico |
topic_facet |
Endophytíc bacteria Bacillus Sweet corn SDS-PAGE rDNA sequencing |
author |
FIGUEIREDO, J. E. F. GOMES, E. A. GUIMARAES, C. T. LANA, U. G. de P. TEIXEIRA, M. A. LIMA, G. V. C. BRESSAN, W. |
author_sort |
FIGUEIREDO, J. E. F. |
title |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
title_short |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
title_full |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
title_fullStr |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
title_full_unstemmed |
Molecular analysis endophytic bacteria from the genus Bacillus isolated from tropical maize (Zea mays L.). |
title_sort |
molecular analysis endophytic bacteria from the genus bacillus isolated from tropical maize (zea mays l.). |
publishDate |
2009-08-05 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/488802 |
work_keys_str_mv |
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