Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa.
Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS).
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2006-09-29
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Subjects: | Cromatografia, Hymenaea Courbaril., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040 |
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dig-alice-doc-150402017-09-19T00:11:49Z Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. NOGUEIRA, R. T. QUEIROZ, S. C. do N. de IMAMURA, P. M. R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp. Cromatografia Hymenaea Courbaril. Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS). 2017-09-19T00:11:49Z 2017-09-19T00:11:49Z 2006-09-29 2002 2017-09-20T11:11:11Z Artigo de periódico Journal of Liquid Chromatography & Related Technologies, New York, v. 25, p 59-67, 2002. http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040 en eng openAccess |
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Cromatografia Hymenaea Courbaril. Cromatografia Hymenaea Courbaril. |
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Cromatografia Hymenaea Courbaril. Cromatografia Hymenaea Courbaril. NOGUEIRA, R. T. QUEIROZ, S. C. do N. de IMAMURA, P. M. Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
description |
Column chromatography of the ethyl acetate extract of the seed pod resin of Hymenaea courbaril var. stilbocarpa furnished a fraction containing a mixture of two new clerodanes and a known clerodane diterpene. Due to the similarity of the structures, they could not be separated by ordinary chromatography even using silica gel impregnated with AgNO 3. Thus, an intense study was made in order to optimize the semipreparative separation of this mixture by HPLC (high performance liquid chromatograph) using several different stationary and mobile phases. The best conditions for separation of these diterpenes was on octadecyl-bonded silica with methanol:water:formic acid (85:15:1, v/v/v) as mobile phase, flow rate at 2 mL/min, and UV detection at 240 nm. The fractions obtained were analyzed by gas chromatography-mass spectrometry (GC-MS). |
author2 |
R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp. |
author_facet |
R. T. NOGUEIRA, IQ/Unicamp; SONIA CLAUDIA DO N DE QUEIROZ, CNPMA; P. M. IMAMURA, IQ/Unicamp. NOGUEIRA, R. T. QUEIROZ, S. C. do N. de IMAMURA, P. M. |
format |
Artigo de periódico |
topic_facet |
Cromatografia Hymenaea Courbaril. |
author |
NOGUEIRA, R. T. QUEIROZ, S. C. do N. de IMAMURA, P. M. |
author_sort |
NOGUEIRA, R. T. |
title |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
title_short |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
title_full |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
title_fullStr |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
title_full_unstemmed |
Semi-preparative HPLC separation of terpenoids from the seed pods of Hymenaea courbaril var. stilbocarpa. |
title_sort |
semi-preparative hplc separation of terpenoids from the seed pods of hymenaea courbaril var. stilbocarpa. |
publishDate |
2006-09-29 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/15040 |
work_keys_str_mv |
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