Induction of somatic embryos in Robusta and Conilon varieties of Coffea canephora.

Clonal propagation of Coffea canephora varieties is feasible through somatic embryogenesis. The aim of this study was to evaluate the induction of somatic embryos from leaves of C. canephora, Robusta and Conilon varieties, in relation to different concentrations of three growth regulators. The explants were inoculated in a MS culture medium with 2iP (isopentanyladenine), IBA (indolebutyric acid) and BA (6-benzylaminopurine), in factorial combinations, at concentrations of 1.0; 2.0; 3.0; 4.0 and 5.0 mg L-1. At 45 days of culture, the percentage of callus induction was evaluated and, at 120 days, the average number of cotyledonary embryos per explant. The most efficient combinations of growth regulators to induce callogenesis in the explants were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 3.0 mg L-1 of IBA + 3. 0 mg L-1 of BA - for the Robusta variety; and 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety. As for the average number of embryos per explant, the most efficient combinations of growth regulators were: 5.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 2.0 mg L-1 of IBA + 3 .0 mg L-1 of BA ? for the Robusta cultivar; and 4.0 mg L-1 of IBA + 1.0 mg L-1 of 2iP and 5.0 mg L-1 of IBA + 5.0 mg L-1 of BA - for the Conilon variety.

Bibliographic Details

Main Authors:	SILVA, C. K. C. da, SANTOS, M. R. A. dos
Other Authors:	CAMILA KAREN CANDEIRA DA SILVA, UNIVERSIDADE FEDERAL DE RONDÔNIA; MAURICIO REGINALDO ALVES DOS SANTOS, CPAF-RO.
Format:	Artigo de periódico biblioteca
Language:	Ingles English
Published:	International Journal of Development Research, v. 12, n. 10, p. 59734-59737, Oct. 2022. 2022-11-16
Subjects:	Cefé Conilon, Cotyledonary embryos, Clonal propagation, Embriogênese somática, Embrapa Rondônia, Porto Velho (RO), Amazônia Ocidental, Western Amazon, Café Robusta, Coffea Canephora, Cultura de Tecido, Campo Experimental, Tissue culture, Somatic embryogenesis, Demonstration farms,
Online Access:	http://www.alice.cnptia.embrapa.br/alice/handle/doc/1148353 https://doi.org/10.37118/ijdr.25620.10.2022
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