Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae.
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future.
Main Authors: | , , , , , , , , , , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | English eng |
Published: |
2019-12-30
|
Subjects: | Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry, Bovine tuberculosis, Mycobacterium bovis BCG, Mycobacterium tuberculosis complex, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117863 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1117863 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-11178632019-12-30T18:11:17Z Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. BACANELLI, G. OLARTE, L. C. SILVA, M. R. RODRIGUES, R. A. CARNEIRO, P. A. M. KANEENE, J. B. PASQUATTI, T. N. TAKATANI, H. ZUMÁRRAGA, M. J. ETGES, R. N. ARAUJO, F. R. VERBISCK, N. V. GISELE BACANELLI, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste RUDIELLE A. RODRIGUES, Universidade Federal de Mato Grosso do Sul - UFMS/Faculdade de Medicina Veterinária/Programa de Pós-Graduação em Ciências Veterinárias PAULO A. M. CARNEIRO, Michigan State University/Center for Comparative Epidemiology JOHN B. KANEENE, Michigan State University/Center for Comparative Epidemiology TAYNARA N. PASQUATTI, Universidade Católica Dom Bosco - UCDB HARUO TAKATANI, Agência de Defesa Agrícola do Amazonas MARTIN J. ZUMÁRRAGA, Institute of Biotechnology, CICVyA/INTA RODRIGO N. ETGES, Secretário de Agricultura, Pecuária e Irrigação de Porto Alegre FLABIO RIBEIRO DE ARAUJO, CNPGC NEWTON VALERIO VERBISCK, CNPGC. LARISSA C. OLARTE, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação Multicêntrica em Bioquímica e Biologia Molecular MARCIO ROBERTO SILVA, CNPGL Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. 2019-12-30T18:11:10Z 2019-12-30T18:11:10Z 2019-12-30 2019 2019-12-30T18:11:10Z Artigo de periódico Journal the Veterinary Medical Science, v. 81, n. 10, p.1400?1408, 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117863 en eng openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
English eng |
topic |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex |
spellingShingle |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex BACANELLI, G. OLARTE, L. C. SILVA, M. R. RODRIGUES, R. A. CARNEIRO, P. A. M. KANEENE, J. B. PASQUATTI, T. N. TAKATANI, H. ZUMÁRRAGA, M. J. ETGES, R. N. ARAUJO, F. R. VERBISCK, N. V. Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
description |
In this study, Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDITOF) mass spectrometry was used to identify Mycobacterium bovis from cattle and buffalo tissue isolates from the North and South regions of Brazil, grown in solid medium and previously identified by Polymerase Chain Reaction (PCR) based on Region of Difference 4 (RD4), sequencing and spoligotyping. For this purpose, the protein extraction protocol and the mass spectra reference database were optimized for the identification of 80 clinical isolates of mycobacteria. As a result of this optimization, it was possible to identify and differentiate M. bovis from other members of the Mycobacterium tuberculosis complex with 100% specificity, 90.91% sensitivity and 91.25% reliability. MALDI-TOF MS methodology described herein provides successful identification of M. bovis within bovine/bubaline clinical samples, demonstrating its usefulness for bovine tuberculosis diagnosis in the future. |
author2 |
GISELE BACANELLI, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste |
author_facet |
GISELE BACANELLI, Universidade Federal de Mato Grosso do Sul - UFMS/Programa de Pós-Graduação em Biotecnologia e Biodiversidade da Região Centro-Oeste BACANELLI, G. OLARTE, L. C. SILVA, M. R. RODRIGUES, R. A. CARNEIRO, P. A. M. KANEENE, J. B. PASQUATTI, T. N. TAKATANI, H. ZUMÁRRAGA, M. J. ETGES, R. N. ARAUJO, F. R. VERBISCK, N. V. |
format |
Artigo de periódico |
topic_facet |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight (MALDI-TOF) mass spectrometry Bovine tuberculosis Mycobacterium bovis BCG Mycobacterium tuberculosis complex |
author |
BACANELLI, G. OLARTE, L. C. SILVA, M. R. RODRIGUES, R. A. CARNEIRO, P. A. M. KANEENE, J. B. PASQUATTI, T. N. TAKATANI, H. ZUMÁRRAGA, M. J. ETGES, R. N. ARAUJO, F. R. VERBISCK, N. V. |
author_sort |
BACANELLI, G. |
title |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
title_short |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
title_full |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
title_fullStr |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
title_full_unstemmed |
Matrix Assisted Laser Desorption Ionization-Time-of-Flight mass spectrometry identification of Mycobacterium bovis in Bovinae. |
title_sort |
matrix assisted laser desorption ionization-time-of-flight mass spectrometry identification of mycobacterium bovis in bovinae. |
publishDate |
2019-12-30 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1117863 |
work_keys_str_mv |
AT bacanellig matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT olartelc matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT silvamr matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT rodriguesra matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT carneiropam matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT kaneenejb matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT pasquattitn matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT takatanih matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT zumarragamj matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT etgesrn matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT araujofr matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae AT verbiscknv matrixassistedlaserdesorptionionizationtimeofflightmassspectrometryidentificationofmycobacteriumbovisinbovinae |
_version_ |
1756026646297575424 |