Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility.
Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr.
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2019-06-19
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Subjects: | Semen quality, Thermotolerance, Touro, Inseminação Artificial, Bulls, In vitro fertilization, Heat stress, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972 |
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dig-alice-doc-11099722023-04-12T13:43:28Z Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. BOTTA, D. ARRUDA, R. P. de WATANABE, Y. F. BALIEIRO, J. C. de C. ROMANELLO, N. BARRETO, A. do N. PANTOJA, M. H. de A. GIRO, A. CARVALHO, C. P. T. de OLIVEIRA, A. de S. GARCIA, A. R. Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE. Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. 2023-04-12T13:43:28Z 2023-04-12T13:43:28Z 2019-06-19 2019 Artigo de periódico Andrologia, v. 51, n. 6, e13266, jul. 2019. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972 10.1111/and.13266 Ingles en openAccess |
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Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress |
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Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress BOTTA, D. ARRUDA, R. P. de WATANABE, Y. F. BALIEIRO, J. C. de C. ROMANELLO, N. BARRETO, A. do N. PANTOJA, M. H. de A. GIRO, A. CARVALHO, C. P. T. de OLIVEIRA, A. de S. GARCIA, A. R. Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
description |
Our aim was to evaluate the effects of three thermal environments over time on kinetics, functionality and in vitro fertility of cryopreserved bovine spermatozoa. Four ejaculates from five bulls (n = 20) were cryopreserved. After thawing, semen was evaluated (0 hr), incubated for 4 hr in T36.0 (36.0°C), T38.0 (38.0°C) and T39.5 (39.5°C), and analysed every hour (1 hr, 2 hr, 3 hr, 4 hr). In vitro production of embryos was performed at 0 hr and 4 hr. Sperm motility and cell kinetics (Computer Assisted Sperm Analysis) were impaired after 2 hr at T38.0 and T39.5 (p < 0.05). Flow cytometry revealed an increase in the cells with injured plasma membrane to 39.5°C and a general reduction in the mitochondrial potential over time (p < 0.05). In vitro fertility was impaired in all temperatures after 4 hr, but there was no difference between 36.0°C and 38.0°C. Our results suggest that the ex situ resilience of semen at 36.0°C after thawing with no major damage to the quality is limited to 3 hr. In normothermia or in thermal stress, sperm cells present a gradual reduction of movement and functionality, which were more significant after 1 hr of incubation. The in vitro production of embryos is impaired when the semen is kept in a thermal environment ≥36.0°C for 4 hr. |
author2 |
Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE. |
author_facet |
Daniela Botta, UFPA; Rubens Paes de Arruda, USP; Yeda Fumie Watanabe, Vitrogen - YVF Biotech; Júlio Cesar de Carvalho Balieiro, USP; Narian Romanello, UFPA; Andréa do Nascimento Barreto, UFPA; Messy Hannear de Andrade Pantoja, UFPA; Alessandro Giro, UFPA; Carla Patrícia Teodoro de Carvalho, USP; Aline de Sousa Oliveira, Vitrogen - YVF Biotech; ALEXANDRE ROSSETTO GARCIA, CPPSE. BOTTA, D. ARRUDA, R. P. de WATANABE, Y. F. BALIEIRO, J. C. de C. ROMANELLO, N. BARRETO, A. do N. PANTOJA, M. H. de A. GIRO, A. CARVALHO, C. P. T. de OLIVEIRA, A. de S. GARCIA, A. R. |
format |
Artigo de periódico |
topic_facet |
Semen quality Thermotolerance Touro Inseminação Artificial Bulls In vitro fertilization Heat stress |
author |
BOTTA, D. ARRUDA, R. P. de WATANABE, Y. F. BALIEIRO, J. C. de C. ROMANELLO, N. BARRETO, A. do N. PANTOJA, M. H. de A. GIRO, A. CARVALHO, C. P. T. de OLIVEIRA, A. de S. GARCIA, A. R. |
author_sort |
BOTTA, D. |
title |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
title_short |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
title_full |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
title_fullStr |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
title_full_unstemmed |
Influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
title_sort |
influence of post-thawing thermal environment on bovine sperm characteristics and in vitro fertility. |
publishDate |
2019-06-19 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1109972 |
work_keys_str_mv |
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1764983905136410624 |