Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci.
The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability.
Main Authors: | , , , , |
---|---|
Other Authors: | |
Format: | Artigo de periódico biblioteca |
Language: | Portugues pt_BR |
Published: |
2018-01-31
|
Subjects: | Veado, Ozotoceros Bezoarticus, Deer, |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086847 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-alice-doc-1086847 |
---|---|
record_format |
koha |
spelling |
dig-alice-doc-10868472023-04-14T13:39:44Z Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília. Veado Ozotoceros Bezoarticus Deer The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. 2023-04-14T13:39:44Z 2023-04-14T13:39:44Z 2018-01-31 2017 Artigo de periódico Genetics and Molecular Biology, v. 40, n. 4, p. 774-780, 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086847 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 Portugues pt_BR openAccess |
institution |
EMBRAPA |
collection |
DSpace |
country |
Brasil |
countrycode |
BR |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-alice |
tag |
biblioteca |
region |
America del Sur |
libraryname |
Sistema de bibliotecas de EMBRAPA |
language |
Portugues pt_BR |
topic |
Veado Ozotoceros Bezoarticus Deer Veado Ozotoceros Bezoarticus Deer |
spellingShingle |
Veado Ozotoceros Bezoarticus Deer Veado Ozotoceros Bezoarticus Deer MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
description |
The pampas deer (Ozotoceros bezoarticus) is close to being classified as 'globally threatened', with the largest population occurring in the Brazilian Pantanal. Since capture is stressful to these animals, non-invasive sampling methods such as the use of feces can provide reliable sources of DNA. The aim of this study was to use fecal samples to evaluate the genetic variability of the Brazilian Pantanal population of pampas deer. Six heterologous microsatellite markers were used to screen 142 stool specimens. Seventy-four deer were identified, of which 50 adults were used to determine the genetic characteristics of the population. The Pantanal population showed high genetic diversity (mean number of alleles per locus = 11.5, expected heterozygosity = 0.75). This is the first investigation to characterize a South American deer species using fecal DNA and demonstrates the usefulness and efficiency of this approach, as well as the feasibility of obtaining information that could not have been easily obtained by traditional DNA sampling. Our findings suggest that management strategies for this species may be much more effective if applied now when the population still shows high genetic variability. |
author2 |
ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília. |
author_facet |
ALINE MEIRA BONFIM MANTELLATTO, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); RENATO CAPARROZ, Universidade de Brasília; MAURÍCIO DURANTE CHRISTOFOLETTI, Universidade Estadual Paulista 'Júlio de Mesquita Filho' (UNESP); UBIRATAN PIOVEZAN, CPATC; JOSÉ MAURÍCIO BARBANTI DUARTE, Universidade de Brasília. MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. |
format |
Artigo de periódico |
topic_facet |
Veado Ozotoceros Bezoarticus Deer |
author |
MANTELLATTO, A. M. B. CAPARROZ, R. CHRISTOFOLETTI, M. D. PIOVEZAN, U. DUARTE, J. M. B. |
author_sort |
MANTELLATTO, A. M. B. |
title |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_short |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_full |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_fullStr |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_full_unstemmed |
Genetic diversity of the pampas deer (Ozotoceros bezoarticus) population in the Brazilian Pantanal assessed by combining fresh fecal DNA analysis and a set of heterologous microsatellite loci. |
title_sort |
genetic diversity of the pampas deer (ozotoceros bezoarticus) population in the brazilian pantanal assessed by combining fresh fecal dna analysis and a set of heterologous microsatellite loci. |
publishDate |
2018-01-31 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1086847 http://dx.doi.org/10.1590/1678-4685-GMB-2016-0323 |
work_keys_str_mv |
AT mantellattoamb geneticdiversityofthepampasdeerozotocerosbezoarticuspopulationinthebrazilianpantanalassessedbycombiningfreshfecaldnaanalysisandasetofheterologousmicrosatelliteloci AT caparrozr geneticdiversityofthepampasdeerozotocerosbezoarticuspopulationinthebrazilianpantanalassessedbycombiningfreshfecaldnaanalysisandasetofheterologousmicrosatelliteloci AT christofolettimd geneticdiversityofthepampasdeerozotocerosbezoarticuspopulationinthebrazilianpantanalassessedbycombiningfreshfecaldnaanalysisandasetofheterologousmicrosatelliteloci AT piovezanu geneticdiversityofthepampasdeerozotocerosbezoarticuspopulationinthebrazilianpantanalassessedbycombiningfreshfecaldnaanalysisandasetofheterologousmicrosatelliteloci AT duartejmb geneticdiversityofthepampasdeerozotocerosbezoarticuspopulationinthebrazilianpantanalassessedbycombiningfreshfecaldnaanalysisandasetofheterologousmicrosatelliteloci |
_version_ |
1764983933159604224 |