Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species.
The timber of the Neotropical tree Cedrela fissilis is used in construction, shipbuilding, carpentry and for medical purposes. In this study, polymorphic microsatellite (SSR) markers derived from an enriched genomic library were characterized using 120 adult trees from four different C. fissilis populations. No substantial genotypic linkage disequilibrium was detected among all possible pairs of SSR loci. The number of alleles per locus ranged from 2 to 20, the average allele number ranged from 8 to 9.7, depending on the population. The observed heterozygosity among the different SSR loci varied from 0.0 to 1.00 , the expected heterozygosity varied from 0.07 to 0.95 On the population level, the average observed and expected heterozygosities ranged from 0.50 to 0.63 and from 0.64 to 0.70, respectively. The average fixation index among populations ranged from 0.09 to 0.24. Thus, the SSR loci revealed high poly - morphism rates and can be used to study the genetic diversity,structure, mating system, and gene flow in C. fissilis.
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Format: | Artigo de periódico biblioteca |
Language: | English eng |
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2018-01-16
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Subjects: | Neotropical tree species., Cedrela Fissilis., Genetic variation, Microsatellite repeats., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085537 |
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dig-alice-doc-10855372018-01-16T23:22:44Z Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. GANDARA, F. B. TAMBARUSSI, E. V. SEBBENN, A. M. FERRAZ, E. M. MORENO, M. A. CIAMPI. A. Y. VIANELLO, R. P. GRATTAPAGLIA, D. KAGEYAMA, P. Y. F. B. GANDARA, ESALQ; E. V. TAMBARUSSI, UNIVERSIDADE FEDERAL DE SÃO CARLOS; A. M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO; E. M. FERRAZ, ESALQ; M. A. MORENO, ESALQ; A. Y. CIAMPI; ROSANA PEREIRA VIANELLO, CNPAF; DARIO GRATTAPAGLIA, Cenargen; P. Y. KAGEYAMA, ESALQ. Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. The timber of the Neotropical tree Cedrela fissilis is used in construction, shipbuilding, carpentry and for medical purposes. In this study, polymorphic microsatellite (SSR) markers derived from an enriched genomic library were characterized using 120 adult trees from four different C. fissilis populations. No substantial genotypic linkage disequilibrium was detected among all possible pairs of SSR loci. The number of alleles per locus ranged from 2 to 20, the average allele number ranged from 8 to 9.7, depending on the population. The observed heterozygosity among the different SSR loci varied from 0.0 to 1.00 , the expected heterozygosity varied from 0.07 to 0.95 On the population level, the average observed and expected heterozygosities ranged from 0.50 to 0.63 and from 0.64 to 0.70, respectively. The average fixation index among populations ranged from 0.09 to 0.24. Thus, the SSR loci revealed high poly - morphism rates and can be used to study the genetic diversity,structure, mating system, and gene flow in C. fissilis. 2018-01-16T23:22:37Z 2018-01-16T23:22:37Z 2018-01-16 2014 2018-01-16T23:22:37Z Artigo de periódico Silvae Genetica, v. 63, n. 5, p. 240-243, 2014. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085537 10.1515/sg-2014-0031 en eng openAccess |
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Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. |
spellingShingle |
Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. GANDARA, F. B. TAMBARUSSI, E. V. SEBBENN, A. M. FERRAZ, E. M. MORENO, M. A. CIAMPI. A. Y. VIANELLO, R. P. GRATTAPAGLIA, D. KAGEYAMA, P. Y. Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
description |
The timber of the Neotropical tree Cedrela fissilis is used in construction, shipbuilding, carpentry and for medical purposes. In this study, polymorphic microsatellite (SSR) markers derived from an enriched genomic library were characterized using 120 adult trees from four different C. fissilis populations. No substantial genotypic linkage disequilibrium was detected among all possible pairs of SSR loci. The number of alleles per locus ranged from 2 to 20, the average allele number ranged from 8 to 9.7, depending on the population. The observed heterozygosity among the different SSR loci varied from 0.0 to 1.00 , the expected heterozygosity varied from 0.07 to 0.95 On the population level, the average observed and expected heterozygosities ranged from 0.50 to 0.63 and from 0.64 to 0.70, respectively. The average fixation index among populations ranged from 0.09 to 0.24. Thus, the SSR loci revealed high poly - morphism rates and can be used to study the genetic diversity,structure, mating system, and gene flow in C. fissilis. |
author2 |
F. B. GANDARA, ESALQ; E. V. TAMBARUSSI, UNIVERSIDADE FEDERAL DE SÃO CARLOS; A. M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO; E. M. FERRAZ, ESALQ; M. A. MORENO, ESALQ; A. Y. CIAMPI; ROSANA PEREIRA VIANELLO, CNPAF; DARIO GRATTAPAGLIA, Cenargen; P. Y. KAGEYAMA, ESALQ. |
author_facet |
F. B. GANDARA, ESALQ; E. V. TAMBARUSSI, UNIVERSIDADE FEDERAL DE SÃO CARLOS; A. M. SEBBENN, INSTITUTO FLORESTAL DE SÃO PAULO; E. M. FERRAZ, ESALQ; M. A. MORENO, ESALQ; A. Y. CIAMPI; ROSANA PEREIRA VIANELLO, CNPAF; DARIO GRATTAPAGLIA, Cenargen; P. Y. KAGEYAMA, ESALQ. GANDARA, F. B. TAMBARUSSI, E. V. SEBBENN, A. M. FERRAZ, E. M. MORENO, M. A. CIAMPI. A. Y. VIANELLO, R. P. GRATTAPAGLIA, D. KAGEYAMA, P. Y. |
format |
Artigo de periódico |
topic_facet |
Neotropical tree species. Cedrela Fissilis. Genetic variation Microsatellite repeats. |
author |
GANDARA, F. B. TAMBARUSSI, E. V. SEBBENN, A. M. FERRAZ, E. M. MORENO, M. A. CIAMPI. A. Y. VIANELLO, R. P. GRATTAPAGLIA, D. KAGEYAMA, P. Y. |
author_sort |
GANDARA, F. B. |
title |
Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
title_short |
Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
title_full |
Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
title_fullStr |
Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
title_full_unstemmed |
Development and characterization of microsatellite loci for Cedrela fissilis Vell (Meliaceae), an endangered tropical tree species. |
title_sort |
development and characterization of microsatellite loci for cedrela fissilis vell (meliaceae), an endangered tropical tree species. |
publishDate |
2018-01-16 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1085537 |
work_keys_str_mv |
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