Impact of fungicide residues on polymerase chain reaction and on yeast metabolism.
The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growth
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Language: | English eng |
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2016-12-27
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Subjects: | PCR inhibitors, Fungicide, Stuck fermentation, Taq polymerase inhibitors., |
Online Access: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059337 |
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dig-alice-doc-10593372017-08-16T03:59:52Z Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. SILVA, G. A. da BERNARDI, T. L. SCHAKER, P. D. C. AGUSTINI, B. C. MELLO, L. M. R. de VALENTE, P. GILDO ALMEIDA DA SILVA, CNPUV Taís Letícia Bernardi, ²Instituto Federal de Educação Ciência e Tecnologia do Rio Grande do Sul, Sertão, Rio Grande do Sul, Brasil Patrícia Dayane Carvalho Schaker, mbrapa Uva e Vinho - Laboratório de Microbiologia, Bento Gonçalves, Brazil BRUNA CARLA AGUSTINI, CNPUV LOIVA MARIA RIBEIRO DE MELLO, CNPUV Patrícia Valente, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brasil. PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growth 2016-12-27T11:11:11Z 2016-12-27T11:11:11Z 2016-12-27 2016 2019-05-06T11:11:11Z Artigo de periódico Brazilian Archives of Biology and Technology, v. 59, e16160241, Jan./Dec. 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059337 en eng openAccess |
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PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. |
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PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. SILVA, G. A. da BERNARDI, T. L. SCHAKER, P. D. C. AGUSTINI, B. C. MELLO, L. M. R. de VALENTE, P. Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
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The indiscriminate use of pesticides on grape crops is harmful for consumers´ healthin “in natura” consumption and in the ingestion of wine and grape juice. During winemaking, a rapid and efficient fermentation stage is critical to avoid proliferation of contaminating microorganisms and to guarantee the product´s quality. Polymerase chain reaction (PCR) has the advantage of detecting these contaminants in the early stages of fermentation. However,this enzymatic reaction may also be susceptible to specific problems, reducing its efficiency. Agricultural practices, such as fungicide treatments, may be a source of PCR inhibiting factors and may also interfere in the normal course of fermentation.The action of the pesticides captan and folpet on PCR and on yeast metabolism was evaluated, once these phthalimide compounds are widely employed in Brazilian vineyards. DNA amplification was only observed at 75 and 37.5 µg/mL of captan concentrations, whereas with folpet, amplification was observed only in the two lowest concentrations tested (42.2 and 21.1µg/mL).Besides the strong inhibition on Taq polymerase activity, phthalimides also inhibited yeast metabolism at all concentrations analyzed.Grape must containing captan and folpet residues could not be transformed into wine due to stuck fermentation caused by the inhibition of yeast metabolism. Noncompliance with the waiting period for phthalimide fungicides may result in financial liabilities to the viticulture sector.The use of yeasts with high fungicide sensitivity should be selected for must fermentation as a strategy for sustainable wine production and to assure that products comply with health and food safety standards. Key words: fungicide;PCR inhibitors; stuck fermentation;Taq polymerase inhibitors; yeast growth |
author2 |
GILDO ALMEIDA DA SILVA, CNPUV |
author_facet |
GILDO ALMEIDA DA SILVA, CNPUV SILVA, G. A. da BERNARDI, T. L. SCHAKER, P. D. C. AGUSTINI, B. C. MELLO, L. M. R. de VALENTE, P. |
format |
Artigo de periódico |
topic_facet |
PCR inhibitors Fungicide Stuck fermentation Taq polymerase inhibitors. |
author |
SILVA, G. A. da BERNARDI, T. L. SCHAKER, P. D. C. AGUSTINI, B. C. MELLO, L. M. R. de VALENTE, P. |
author_sort |
SILVA, G. A. da |
title |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
title_short |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
title_full |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
title_fullStr |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
title_full_unstemmed |
Impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
title_sort |
impact of fungicide residues on polymerase chain reaction and on yeast metabolism. |
publishDate |
2016-12-27 |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1059337 |
work_keys_str_mv |
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1756022980130897920 |