Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).

Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).

Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.

Saved in:
Bibliographic Details
Main Authors: NASCIMENTO, A. R., SILVA, G. F., GUALBERTO, G. F., ALMEIDA, F. L.
Other Authors: A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
Format: Separatas biblioteca
Language:English
eng
Published: 2016-12-07
Subjects:Colossoma Macropomum, Tambaqui., aquaculture, molecular biology.,
Online Access:http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147
http://dx.doi.org/10.4238/gmr15049228
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-alice-doc-1058147
record_format koha
spelling dig-alice-doc-10581472017-08-16T04:03:32Z Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum). NASCIMENTO, A. R. SILVA, G. F. GUALBERTO, G. F. ALMEIDA, F. L. A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA. Colossoma Macropomum Tambaqui. aquaculture molecular biology. Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR. 2016-12-07T11:11:11Z 2016-12-07T11:11:11Z 2016-12-07 2016 2020-03-31T11:11:11Z Separatas Genetics and Molecular Research, v. 15, n. 4, p. 15049228, 2016. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147 http://dx.doi.org/10.4238/gmr15049228 en eng openAccess
institution EMBRAPA
collection DSpace
country Brasil
countrycode BR
component Bibliográfico
access En linea
databasecode dig-alice
tag biblioteca
region America del Sur
libraryname Sistema de bibliotecas de EMBRAPA
language English
eng
topic Colossoma Macropomum
Tambaqui.
aquaculture
molecular biology.
Colossoma Macropomum
Tambaqui.
aquaculture
molecular biology.
spellingShingle Colossoma Macropomum
Tambaqui.
aquaculture
molecular biology.
Colossoma Macropomum
Tambaqui.
aquaculture
molecular biology.
NASCIMENTO, A. R.
SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
description Tambaqui, Colossoma macropomum, is the main native freshwater fish in Brazilian aquaculture. Therefore, intensive research pressure has been applied to the species to support new technologies for tambaqui farming. Molecular biology represents a tool that can be used to investigate every field of applied biology, from fish physiology to the effects of climate change. Based on the importance of reference genes for the relative or absolute quantification of gene transcripts, we cloned and sequenced three candidate reference genes in tambaqui, and validated a set of primers for each gene for use in real-time quantitative PCR.
author2 A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
author_facet A. R. Nascimento, Faculdade Estácio do Amazonas; GILVAN FERREIRA DA SILVA, CPAA; G. F. Gualberto; FERNANDA LOUREIRO ALMEIDA OSULLIVAN, CPAA.
NASCIMENTO, A. R.
SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
format Separatas
topic_facet Colossoma Macropomum
Tambaqui.
aquaculture
molecular biology.
author NASCIMENTO, A. R.
SILVA, G. F.
GUALBERTO, G. F.
ALMEIDA, F. L.
author_sort NASCIMENTO, A. R.
title Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_short Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_full Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_fullStr Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_full_unstemmed Validation of reference genes for real-time quantitative PCR in tambaqui (Colossoma macropomum).
title_sort validation of reference genes for real-time quantitative pcr in tambaqui (colossoma macropomum).
publishDate 2016-12-07
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1058147
http://dx.doi.org/10.4238/gmr15049228
work_keys_str_mv AT nascimentoar validationofreferencegenesforrealtimequantitativepcrintambaquicolossomamacropomum
AT silvagf validationofreferencegenesforrealtimequantitativepcrintambaquicolossomamacropomum
AT gualbertogf validationofreferencegenesforrealtimequantitativepcrintambaquicolossomamacropomum
AT almeidafl validationofreferencegenesforrealtimequantitativepcrintambaquicolossomamacropomum
_version_ 1756023042584084480

Similar Items