CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum

In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides.In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides.

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Bibliographic Details
Main Author: University of Kentucky (17855297)
Format: Dataset biblioteca
Published: 2017
Subjects:Genetics, Tribolium castaneum, QTC279, eEukaryotes, raw sequence reads,
Online Access:https://figshare.com/articles/dataset/CncC_regulated_genes_code_for_proteins_involved_in_all_three_phases_of_insecticide_detoxification_in_Tribolium_Castaneum/25082177
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spelling dat-usda-us-article250821772017-04-17T00:00:00Z CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum University of Kentucky (17855297) Genetics Tribolium castaneum QTC279 eEukaryotes raw sequence reads In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides.In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides. 2017-04-17T00:00:00Z Dataset Dataset 10113/AF25082177 https://figshare.com/articles/dataset/CncC_regulated_genes_code_for_proteins_involved_in_all_three_phases_of_insecticide_detoxification_in_Tribolium_Castaneum/25082177 Copyright Undetermined
institution USDA US
collection Figshare
country Estados Unidos
countrycode US
component Datos de investigación
access En linea
databasecode dat-usda-us
tag biblioteca
region America del Norte
libraryname National Agricultural Library of USDA
topic Genetics
Tribolium castaneum
QTC279
eEukaryotes
raw sequence reads
spellingShingle Genetics
Tribolium castaneum
QTC279
eEukaryotes
raw sequence reads
University of Kentucky (17855297)
CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
description In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides.In invertebrates, a heterodimer of xenobiotic transcription factors, cap n collar C isoform (CncC) and muscle aponeurosis fibromatosis (Maf) mediate cellular defense. In insects, these proteins regulate expression of genes involved in insecticide detoxification. In the current study, we performed sequencing of RNA isolated from Tribolium castaneum pyrethriod resistant strain (QTC279) beetles injected with CncC or green fluroscence protein (GFP, control) dsRNA. Differential expression analysis of RNA sequences identified 662 genes that showed a decrease and 91 genes that showed an increase in expression (at a p value 1.5) in CncC knockdown insects when compared to their expression in control insects. We focused on the downregulated genes and selected a subset of 27 genes (21 with a predicted function in xenobiotic detoxification and six randomly picked) and verified their differential expression using qRT-PCR. RNAi and insecticide bioassays were employed to study the function of six of these genes coding for CYP4G7, CYP4G14, GST-1 and four ABC transporters, ABCA-UB, ABCA-A1 and ABCA-A1L and ABCA-9B involved in all three phases of insecticide detoxification. These data suggest that CncC is a major regulator of genes coding for proteins involved in detoxification of insecticides.
format Dataset
author University of Kentucky (17855297)
author_facet University of Kentucky (17855297)
author_sort University of Kentucky (17855297)
title CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
title_short CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
title_full CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
title_fullStr CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
title_full_unstemmed CncC regulated genes code for proteins involved in all three phases of insecticide detoxification in Tribolium Castaneum
title_sort cncc regulated genes code for proteins involved in all three phases of insecticide detoxification in tribolium castaneum
publishDate 2017
url https://figshare.com/articles/dataset/CncC_regulated_genes_code_for_proteins_involved_in_all_three_phases_of_insecticide_detoxification_in_Tribolium_Castaneum/25082177
work_keys_str_mv AT universityofkentucky17855297 cnccregulatedgenescodeforproteinsinvolvedinallthreephasesofinsecticidedetoxificationintriboliumcastaneum
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